利用高效液相色谱-串联质谱法同时测定剑南春混合物中的 19 种成分

IF 1.3 Q4 CHEMISTRY, ANALYTICAL
Ji‐Yong Fu, Ding Ding, Ru‐Mei Wang, Lyu‐Feng Zhang, Bing‐Liang Ma, Fu‐Xuan Liu, Guo‐Fu Zhu
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引用次数: 0

摘要

在中国,金萘宁混合物(JNNm)一直被临床用于治疗失眠。然而,金萘宁钠混合物的主要成分并未被揭示。本研究建立了超高效液相色谱-串联质谱法,并对其进行了验证。该方法具有良好的特异性和足够的灵敏度。在适当的范围内,各成分的校准曲线显示出良好的线性关系(r > 0.990)。在三个质控水平下,日内和日间精密度分别为 0.68% 至 5.17% 和 0.42% 至 5.37%。回收率为 91.76% 至 110.06%,精密度小于 5.07%。此外,该方法的重复性良好(相对标准偏差 [RSD] < 4.93%)。分析物在室温下均能稳定地保存 24 小时(RSD < 7.98%)。定量分析结果表明,丹酚酸 B(495.7-606.3 μg/mL)是含量最高的成分,其次是甜菜碱(411.3-468.3 μg/mL)、丹酚酸 A(10.7-34.9 μg/mL)、龙脑叶乙素(11.6-14.6 μg/mL)、旋花素(4.0-7.6 μg/mL)、五味子醇 A(1.5-4.1 μg/mL)和五味子醇 B(1.2-1.5 μg/mL)。其他成分的浓度低于 1 微克/毫升。这项研究将有助于对 JNNm 进行质量控制并揭示其有效成分。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Simultaneous determination of 19 constituents in the Jian‐Nao‐Ning mixture using high‐performance liquid chromatography‐tandem mass spectrometry
Jin‐Nao‐Ning mixture (JNNm) has been clinically used to treat insomnia in China. However, the major constituents of JNNm were not revealed. In this study, an ultra‐high‐performance liquid chromatography‐tandem mass spectrometry was established and validated for simultaneous quantitation of 19 representative constituents of JNNm. The method had good specificity and adequate sensitivity. The calibration curves of the constituents displayed good linearity (r > 0.990) in appropriate ranges. The intra‐ and inter‐day precision at three quality control (QC) levels ranged from 0.68% to 5.17% and 0.42% to 5.37%, respectively. The recovery ranged from 91.76% to 110.06%, with a precision of less than 5.07%. In addition, the method had good repeatability (relative standard deviation [RSD] < 4.93%). The analytes were all stable for 24 h (RSD < 7.98%) at room temperature. The results of the quantitative analysis showed that salvianolic acid B (495.7–606.3 μg/mL) is the most abundant constituent, followed by betaine (411.3–468.3 μg/mL), salvianic acid A (10.7–34.9 μg/mL), lobetyolin (11.6–14.6 μg/mL), spinosin (4.0–7.6 μg/mL), schisandrol A (1.5–4.1 μg/mL), and schisandrol B (1.2–1.5 μg/mL). Concentrations of other constituents were below 1 μg/mL. This study would be helpful for the QC of JNNm and the revelation of its effective constituents.
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来源期刊
SEPARATION SCIENCE PLUS
SEPARATION SCIENCE PLUS CHEMISTRY, ANALYTICAL-
CiteScore
1.90
自引率
9.10%
发文量
111
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