Kangqi Zhao, Ming Hao, Qian Xu, Hong-Xue Li, Cheng-Ye Xu, Ziyu Meng, H. Kuang
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引用次数: 0
摘要
随着高通量测序技术的发展,人类已经能够对 DNA 序列、染色质结构、RNA 转录本、蛋白质、代谢物等基因组及其产物进行大规模分析。传统的基于组织样本的高通量转录组测序技术(RNA Seq)用于对成千上万个细胞进行集中测序,每个细胞的大小、蛋白质水平和 mRNA 表达转录各不相同。测量多个细胞的平均值会掩盖细胞间基因表达的显著差异。单细胞 RNA 测序是一种在单细胞水平对基因组、转录组和表观基因组进行高通量测序的技术。根据单细胞RNA转录图谱,可将眼内细胞与其他亚型细胞区分开来,并发现不同亚型细胞在形态、生理和特异性表达基因方面存在显著差异。近年来,单细胞RNA测序技术在眼科领域的应用越来越多,主要包括细胞类型和细胞亚型鉴定、视网膜发育过程和眼科疾病研究等。本文系统总结了单细胞测序技术在糖尿病视网膜病变领域的最新应用,归纳了标志基因和潜在治疗靶点。对糖尿病视网膜病变的临床治疗具有指导意义。
Current Advances in Single-Cell RNA Sequencing in Diabetic Retinopathy
With the development of high-throughput sequencing technology, humans have been able to conduct large-scale analysis of DNA sequence, chromatin structure, RNA transcripts, proteins, metabolites and other genomes and their products. Traditional high-throughput transcriptome sequencing
techniques based on tissue samples (RNA Seq) are used to centrally sequence thousands of cells, each of which varies in size, protein levels, and mRNA expression transcription. Measuring the average of multiple cells grouped together can mask significant differences in gene expression between
cells. Single-cell RNA sequencing is a technique for high-throughput sequencing of the genome, transcriptome, and epigenome at the single-cell level. Based on the single cell RNA transcription map, the intraocular cells can be distinguished from other subtypes, and the different subtypes are
found to have significant differences in morphology, physiology and specific expression genes. In recent years, the application of single-cell RNA sequencing technology in the field of ophthalmology has increased, mainly including cell type and cell subtype identification, retinal development
process, and eye disease research. This paper systematically summarized the latest application of single-cell sequencing technology in the field of diabetic retinopathy, and summarized marker genes and potential therapeutic targets. It has guiding significance for the clinical treatment of
diabetic retinopathy.