Ji Ma, Yunna Ning, Ke Lu, Hui Wang, Ping Li, Lili Feng, Jianing Zhang, Linna Xie, Qiang He
{"title":"HLA-G 基因 5′ UTR 中 CpG 岛的高甲基化会降低免疫性血小板减少症患者的 HLA-G 基因表达量","authors":"Ji Ma, Yunna Ning, Ke Lu, Hui Wang, Ping Li, Lili Feng, Jianing Zhang, Linna Xie, Qiang He","doi":"10.1166/jbn.2024.3767","DOIUrl":null,"url":null,"abstract":"This study investigated the impact of DNA methylation in the 5′ untranslated region-CpG island (5′ UTR) of the HLA-G gene on soluble HLA-G (sHLA-G) levels in immune thrombocytopenia (ITP) patients, shedding light on sHLA-G’s regulatory mechanisms in ITP. Using a cohort\n of 53 participants, including ITP patients, DNA methylation profiles in the HLA-G gene’s 5′ UTR were analyzed with Sequenom MassARRAY Methylation Analysis. sHLA-G levels were measured by enzyme-linked immunosorbent assay, and platelet antibodies were assessed using modified MAIPA.\n Results showed increased DNA methylation at specific CpG sites (CpG3, CpG18, CpG19, and CpG20.21) in ITP patients. A negative correlation between DNA methylation and sHLA-G expression, particularly at CpG18, was found. Patients with Anti-GPIb/IX antibodies had higher CpG18 methylation. Age\n and gender didn’t correlate significantly with methylation. This underscores 5′ UTR hypermethylation’s role in influencing circulating HLA-G levels, revealing insights into ITP development and potential therapeutic targets. By linking DNA methylation to sHLA-G expression,\n this advances ITP understanding, suggesting new therapeutic strategies.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9000,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Hyper-Methylation of CpG Island in 5′ UTR of the HLA-G Gene Reduces Its Expression in Individuals with Immune Thrombocytopenia\",\"authors\":\"Ji Ma, Yunna Ning, Ke Lu, Hui Wang, Ping Li, Lili Feng, Jianing Zhang, Linna Xie, Qiang He\",\"doi\":\"10.1166/jbn.2024.3767\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"This study investigated the impact of DNA methylation in the 5′ untranslated region-CpG island (5′ UTR) of the HLA-G gene on soluble HLA-G (sHLA-G) levels in immune thrombocytopenia (ITP) patients, shedding light on sHLA-G’s regulatory mechanisms in ITP. Using a cohort\\n of 53 participants, including ITP patients, DNA methylation profiles in the HLA-G gene’s 5′ UTR were analyzed with Sequenom MassARRAY Methylation Analysis. sHLA-G levels were measured by enzyme-linked immunosorbent assay, and platelet antibodies were assessed using modified MAIPA.\\n Results showed increased DNA methylation at specific CpG sites (CpG3, CpG18, CpG19, and CpG20.21) in ITP patients. A negative correlation between DNA methylation and sHLA-G expression, particularly at CpG18, was found. Patients with Anti-GPIb/IX antibodies had higher CpG18 methylation. Age\\n and gender didn’t correlate significantly with methylation. This underscores 5′ UTR hypermethylation’s role in influencing circulating HLA-G levels, revealing insights into ITP development and potential therapeutic targets. By linking DNA methylation to sHLA-G expression,\\n this advances ITP understanding, suggesting new therapeutic strategies.\",\"PeriodicalId\":15260,\"journal\":{\"name\":\"Journal of biomedical nanotechnology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2024-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of biomedical nanotechnology\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1166/jbn.2024.3767\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of biomedical nanotechnology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1166/jbn.2024.3767","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Medicine","Score":null,"Total":0}
Hyper-Methylation of CpG Island in 5′ UTR of the HLA-G Gene Reduces Its Expression in Individuals with Immune Thrombocytopenia
This study investigated the impact of DNA methylation in the 5′ untranslated region-CpG island (5′ UTR) of the HLA-G gene on soluble HLA-G (sHLA-G) levels in immune thrombocytopenia (ITP) patients, shedding light on sHLA-G’s regulatory mechanisms in ITP. Using a cohort
of 53 participants, including ITP patients, DNA methylation profiles in the HLA-G gene’s 5′ UTR were analyzed with Sequenom MassARRAY Methylation Analysis. sHLA-G levels were measured by enzyme-linked immunosorbent assay, and platelet antibodies were assessed using modified MAIPA.
Results showed increased DNA methylation at specific CpG sites (CpG3, CpG18, CpG19, and CpG20.21) in ITP patients. A negative correlation between DNA methylation and sHLA-G expression, particularly at CpG18, was found. Patients with Anti-GPIb/IX antibodies had higher CpG18 methylation. Age
and gender didn’t correlate significantly with methylation. This underscores 5′ UTR hypermethylation’s role in influencing circulating HLA-G levels, revealing insights into ITP development and potential therapeutic targets. By linking DNA methylation to sHLA-G expression,
this advances ITP understanding, suggesting new therapeutic strategies.