HLA-G 基因 5′ UTR 中 CpG 岛的高甲基化会降低免疫性血小板减少症患者的 HLA-G 基因表达量

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Ji Ma, Yunna Ning, Ke Lu, Hui Wang, Ping Li, Lili Feng, Jianing Zhang, Linna Xie, Qiang He
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引用次数: 0

摘要

本研究调查了免疫性血小板减少症(ITP)患者体内 HLA-G 基因 5′ 非翻译区-CpG 岛(5′ UTR)DNA 甲基化对可溶性 HLA-G (sHLA-G)水平的影响,从而揭示了 sHLA-G 在 ITP 中的调控机制。利用包括ITP患者在内的53名参与者组成的队列,采用Sequenom MassARRAY甲基化分析仪分析了HLA-G基因5′UTR的DNA甲基化图谱。结果显示,ITP 患者特定 CpG 位点(CpG3、CpG18、CpG19 和 CpG20.21)的 DNA 甲基化增加。DNA 甲基化与 sHLA-G 表达呈负相关,尤其是在 CpG18 位点。抗 GPIb/IX 抗体患者的 CpG18 甲基化程度较高。年龄和性别与甲基化无明显相关性。这强调了5′UTR高甲基化在影响循环HLA-G水平中的作用,揭示了ITP的发展和潜在的治疗靶点。通过将DNA甲基化与sHLA-G表达联系起来,这加深了人们对ITP的了解,并提出了新的治疗策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Hyper-Methylation of CpG Island in 5′ UTR of the HLA-G Gene Reduces Its Expression in Individuals with Immune Thrombocytopenia
This study investigated the impact of DNA methylation in the 5′ untranslated region-CpG island (5′ UTR) of the HLA-G gene on soluble HLA-G (sHLA-G) levels in immune thrombocytopenia (ITP) patients, shedding light on sHLA-G’s regulatory mechanisms in ITP. Using a cohort of 53 participants, including ITP patients, DNA methylation profiles in the HLA-G gene’s 5′ UTR were analyzed with Sequenom MassARRAY Methylation Analysis. sHLA-G levels were measured by enzyme-linked immunosorbent assay, and platelet antibodies were assessed using modified MAIPA. Results showed increased DNA methylation at specific CpG sites (CpG3, CpG18, CpG19, and CpG20.21) in ITP patients. A negative correlation between DNA methylation and sHLA-G expression, particularly at CpG18, was found. Patients with Anti-GPIb/IX antibodies had higher CpG18 methylation. Age and gender didn’t correlate significantly with methylation. This underscores 5′ UTR hypermethylation’s role in influencing circulating HLA-G levels, revealing insights into ITP development and potential therapeutic targets. By linking DNA methylation to sHLA-G expression, this advances ITP understanding, suggesting new therapeutic strategies.
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
期刊介绍: ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.
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