利用放射性同位素评估兽药损耗的新方法

IF 1.4 3区 化学 Q4 CHEMISTRY, INORGANIC & NUCLEAR
Yousef Fazaeli, Gholamreza Shahhoseini, Alireza Neisi, James Jacob Sasanya, Parviz Ashtari, Shahzad Feizi
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引用次数: 0

摘要

抗菌药耐药性(AMR)是全球最紧迫的公共卫生问题之一,需要立即引起重视,因为它有可能影响人类医疗保健、农业和兽医行业。尽管有关于过度使用抗菌药及其影响的警告,但全世界仍在为人类和动物过度使用抗菌药,从而导致细菌等抗药性微生物的滋生。食品是接触或转移药物残留的媒介,可加重与 AMR 发展相关的药物负担。研究食品中的兽药残留对抗击 AMR 至关重要。在此,我们介绍一种利用放射性核素观察动物基质中兽药残留的新方法,称为 "损耗成像"。阿莫西林被选为本研究的第一种抗菌素。该药物用 [62Zn/65Zn] ZnCl2 标记。同时给虹鳟鱼注射放射性标记的阿莫西林和未标记的阿莫西林。为了观察阿莫西林在鱼体内的残留情况,在用药后 30 分钟至 21 天的不同时间间隔进行了体内正电子发射断层扫描(PET)成像。使用高纯锗(HPGe)核检测器评估鱼体内放射性标记阿莫西林的含量,使用酶联免疫吸附试验(ELISA)技术检测非标记药物。这项研究还包括一种内部生产锌放射性同位素的综合方法。研究结果表明,基于组织中放射性核素伽马能谱的耗竭成像和生物分布研究是准确了解药物分布、代谢和排泄情况的精确方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A novel method for evaluating the depletion of veterinary pharmaceuticals using radioisotopes
Antimicrobial resistance (AMR) as one of the world’s most pressing public health problems needs immediate attention, because it has the ability to affect the human healthcare, agriculture, and veterinary industries. Despite warnings about overuse and their implications, antimicrobials are overprescribed worldwide for humans and animals, which leads to the promotion of resistant microorganisms such as bacteria. Food is a medium for exposure to or transfer of residues of the drugs and can contribute to the burden of the pharmaceuticals associated with development of AMR. Studying on residues of veterinary drugs in foods is essential in the fight against AMR. Herein, we introduce a new method for visualizing the residues of a veterinary drug in animal matrices using radionuclides, called “Depletion Imaging”. Amoxicillin was chosen to be the first antimicrobial for this study. The drug was labeled with [62Zn/65Zn] ZnCl2. Radiolabelled amoxicillin and non-labeled amoxicillin were administrated to rainbow trout fish simultaneously. To enable visualization of the remaining residues of amoxicillin in fish, In-vivo positron emission tomography (PET) imaging was done at different intervals from 30 min to 21 days after administration. Evaluation of the amount of radiolabelled amoxicillin in fish was done using a high purity germanium (HPGe) nuclear detector, and enzyme linked immunosorbent assay (ELISA) technique was used for the non-labeled drug. In this study, a comprehensive method for in-house production of zinc radioisotopes was also included. The results showed that depletion imaging and biodistribution study based on gamma spectroscopy of radionuclides in tissues, is a precise method for accurate understanding of the drug’s distribution, metabolic and excretory profile.
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来源期刊
Radiochimica Acta
Radiochimica Acta 化学-核科学技术
CiteScore
2.90
自引率
16.70%
发文量
78
审稿时长
6 months
期刊介绍: Radiochimica Acta publishes manuscripts encompassing chemical aspects of nuclear science and technology.
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