利用可调纳米粒子表面功能化改变细胞迁移

IF 4.8 Q2 NANOSCIENCE & NANOTECHNOLOGY
Maxwell G. Tetrick,  and , Catherine J. Murphy*, 
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引用次数: 0

摘要

金纳米粒子(AuNPs)是一种前景广阔的生物医学应用平台,包括治疗、成像和给药。有关将 AuNPs 引入细胞系统的文献大多集中在吸收和细胞毒性方面,而对于 AuNPs 如何通过与细胞外环境的相互作用间接影响细胞则了解较少。以前的研究表明,单核细胞系 THP-1 在单核细胞趋化蛋白 1(MCP-1)梯度作用下的趋化能力会受到细胞外多磺化 AuNPs 的影响,这可能是由于 AuNPs 与 MCP-1 的结合比与介质中的其他蛋白结合更有偏好。这项工作要探讨的假设是,表面的磺化程度将因此与 AuNPs 干扰趋化性的能力相关联。高磺化聚(苯乙烯磺酸盐)包覆的 AuNPs 对 THP-1 的趋化有很强的抑制作用;通过使用共聚物[不同成分的聚(苯乙烯磺酸盐-共马来酸盐)]降低 AuNPs 表面的磺化程度,发现中等和低磺化程度的 AuNPs 对 THP-1 的趋化分别有微弱和无抑制作用。小分子、硬质磺酸盐表面的趋化抑制作用相对较弱。与众不同的是,游离聚(苯乙烯磺酸盐)会导致 THP-1 细胞迁移的剂量依赖性逆转:在低浓度下,游离聚(苯乙烯磺酸盐)会显著抑制 MCP-1 诱导的趋化。然而,在高浓度下,游离聚(苯乙烯磺酸盐)会起到趋化作用,导致细胞迁移方向逆转。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Leveraging Tunable Nanoparticle Surface Functionalization to Alter Cellular Migration

Leveraging Tunable Nanoparticle Surface Functionalization to Alter Cellular Migration

Leveraging Tunable Nanoparticle Surface Functionalization to Alter Cellular Migration

Gold nanoparticles (AuNPs) are a promising platform for biomedical applications including therapeutics, imaging, and drug delivery. While much of the literature surrounding the introduction of AuNPs into cellular systems focuses on uptake and cytotoxicity, less is understood about how AuNPs can indirectly affect cells via interactions with the extracellular environment. Previous work has shown that the monocytic cell line THP-1’s ability to undergo chemotaxis in response to a gradient of monocyte chemoattractant protein 1 (MCP-1) was compromised by extracellular polysulfonated AuNPs, presumably by binding to MCP-1 with some preference over other proteins in the media. The hypothesis to be explored in this work is that the degree of sulfonation of the surface would therefore be correlated with the ability of AuNPs to interrupt chemotaxis. Highly sulfonated poly(styrenesulfonate)-coated AuNPs caused strong inhibition of THP-1 chemotaxis; by reducing the degree of sulfonation on the AuNP surface with copolymers [poly(styrenesulfonate-co-maleate) of different compositions], it was found that medium and low sulfonation levels caused weak to no inhibition, respectively. Small, rigid molecular sulfonate surfaces were relatively ineffective at chemotaxis inhibition. Unusually, free poly(styrenesulfonate) caused a dose-dependent reversal of THP-1 cell migration: at low concentrations, free poly(styrenesulfonate) significantly inhibited MCP-1-induced chemotaxis. However, at high concentrations, free poly(styrenesulfonate) acted as a chemorepellent, causing a reversal in the cell migration direction.

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来源期刊
ACS Nanoscience Au
ACS Nanoscience Au 材料科学、纳米科学-
CiteScore
4.20
自引率
0.00%
发文量
0
期刊介绍: ACS Nanoscience Au is an open access journal that publishes original fundamental and applied research on nanoscience and nanotechnology research at the interfaces of chemistry biology medicine materials science physics and engineering.The journal publishes short letters comprehensive articles reviews and perspectives on all aspects of nanoscience and nanotechnology:synthesis assembly characterization theory modeling and simulation of nanostructures nanomaterials and nanoscale devicesdesign fabrication and applications of organic inorganic polymer hybrid and biological nanostructuresexperimental and theoretical studies of nanoscale chemical physical and biological phenomenamethods and tools for nanoscience and nanotechnologyself- and directed-assemblyzero- one- and two-dimensional materialsnanostructures and nano-engineered devices with advanced performancenanobiotechnologynanomedicine and nanotoxicologyACS Nanoscience Au also publishes original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials engineering physics bioscience and chemistry into important applications of nanomaterials.
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