利用巴西鹅膏菌优化银纳米粒子的生物形成

IF 1.3 4区生物学 Q4 MICROBIOLOGY

Microbiology Pub Date : 2024-02-07 DOI:10.1134/s002626172360338x

M. A. Kupryashina, E. P. Vetchinkina, A. S. Abdrakhmanova, E. G. Ponomareva, T. E. Pylaev, V. A. Khanadeev, A. M. Burov

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引用次数: 0

摘要

摘要--本文介绍了利用非致病性土壤细菌巴西天青霉（Azospirillum brasilense）进行银纳米粒子生物合成的优化结果。分析了利用巴西银杆菌无性细胞、培养液和均相多酚氧化酶制剂合成生物纳米粒子的条件和参数。我们揭示了培养时间、银离子浓度、反应混合物的 pH 值和代谢物浓度对生产具有特定几何参数、高胶体稳定性和相对较好的单分散性的胶体银纳米粒子的影响。透射电子显微镜和动态光散射测定了电子致密纳米结构的尺寸、形状和相对产量；紫外-可见分光光度法记录了消光光谱。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Optimization of Bioformation of Silver Nanoparticles Using Azospirillum brasilense Cultures

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Optimization of Bioformation of Silver Nanoparticles Using Azospirillum brasilense Cultures

Abstract—

Herein we present the results of optimization of silver nanoparticles biosynthesis using a nonpathogenic soil bacterium Azospirillum brasilense. The conditions and parameters for synthesis of biogenic nanoparticles using A. brasilense vegetative cells, culture liquid, and homogeneous polyphenol oxidase preparations were analyzed. We have revealed the ranging effect of the incubation time, the concentration of silver ions, pH of the reaction mixture, and the concentration of metabolites, towards the production of colloidal silver nanoparticles with specified geometric parameters, high colloidal stability and relatively good monodispersity. The size, shape, and relative yield of electron-dense nanostructures were determined by transmission electron microscopy and dynamic light scattering; extinction spectra were recorded by UV-vis spectrophotometry.

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来源期刊

Microbiology 生物-微生物学

CiteScore

2.40

自引率

13.30%

发文量

审稿时长

6-12 weeks

期刊介绍： Microbiology is an is an international peer reviewed journal that covers a wide range of problems in the areas of fundamental and applied microbiology. The journal publishes experimental and theoretical papers, reviews on modern trends in different fields of microbiological science, and short communications with descriptions of unusual observations. The journal welcomes manuscripts from all countries in the English or Russian language.