Thiocapsa bogorovii BBS 的硫代谢和 HydSL 氢酶的作用

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-02-07 DOI:10.1134/s002626172360386x

M. Kh. Khasimov, E. P. Petushkova, O. P. Serdyuk, A. A. Tsygankov

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引用次数: 0

摘要

摘要迄今为止，我们只在原生细胞中观察到 T. bogorovii BBS 的 HydSL 氢酶催化硫还原为硫化氢的特定酶促反应。在这项工作中，我们能够在这些细菌的细胞匀浆中检测到依赖氢的反应 H2 + S0 → H2S + HS- + H+。这是通过在高离子强度的优化缓冲液中破坏细胞实现的。进一步纯化制备物后，其比活度提高了 28 倍。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Sulfur Metabolism in Thiocapsa bogorovii BBS and the Role of HydSL Hydrogenase

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Sulfur Metabolism in Thiocapsa bogorovii BBS and the Role of HydSL Hydrogenase

Abstract—

The specific enzymatic reaction of sulfur reduction to hydrogen sulfide catalyzed by the HydSL hydrogenase of T. bogorovii BBS has so far been observed only in native cells. In this work, we were able to detect the hydrogen-dependent reaction H₂ + S⁰ → H₂S + HS^– + H⁺ in a cell homogenate of these bacteria. This was achieved by destroying the cells in an optimized buffer of high ionic strength. Further purification of the preparation led to a 28-fold increase in its specific activity.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.