hps/phi 拷贝数对荚膜甲球菌 MIR 生长的影响

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-12-01 DOI:10.1134/s0026261723603536

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引用次数: 0

摘要

摘要- Methylococcus capsulatus MIR 是一株具有生物技术前景的甲烷营养细菌，可从甲烷中获取饲料蛋白和其他增值产品。该菌株主要通过核酮糖单磷酸途径同化甲烷碳，其关键酶为 3-己酮糖-6-磷酸合成酶和 6-磷酸-3-己酮糖异构酶。研究表明，编码磷酸己糖合成酶/异构酶基因的一个、两个或三个拷贝的存在会影响总基因的表达水平，但不会影响菌株的生长特性，也与细胞中的蛋白质含量无关。获得的数据表明，磷酸己糖合成酶固定甲醛并不是限制甲基球菌属细菌生长的反应。

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Effect of the hps/phi Copy Number on the Growth of Methylococcus capsulatus MIR

Abstract—

Methylococcus capsulatus MIR is a biotechnologically promising strain of methanotrophic bacteria for obtaining feed protein and other value-added products from methane. This strain assimilates methane carbon mainly via the ribulose monophosphate pathway, the key enzymes of which are 3-hexulose-6-phosphate synthase and 6-phospho-3-hexulose isomerase. It was shown that the presence of one, two, or three copies of the genes encoding hexulose phosphate synthase/isomerase affected the level of the total gene expression but did not affect the growth characteristics of the strain and did not correlate with the protein content in the cells. The data obtained indicate that fixation of formaldehyde by hexulose phosphate synthase is not the reaction limiting the growth of bacteria of the genus Methylococcus.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.