野香蕉(Musa acuminata ssp.)胚胎细胞悬浮培养的诱导、生长和特征

IF 2.2 3区 生物学 Q4 CELL BIOLOGY
Tri Handayani, Awang Maharijaya, Yudiwanti Wahyu, Sobir, Witjaksono
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引用次数: 0

摘要

野生香蕉及其近缘种因其遗传多样性、抗病性、对非生物胁迫的耐受性以及其他理想性状,有可能被用于育种前研究。野生香蕉的胚胎悬浮培养为利用这种遗传多样性进行香蕉遗传改良提供了一种手段。本文阐明了不同亚种 M. acuminata(ssp. malaccensis、microcarpa、sumatrana 和 breviformis)对悬浮培养物的诱导、生长和行为及其再生为小植株的反应。从胚胎培养诱导阶段、培养增殖到小植株形成,不同亚种表现出不同的反应。通过体细胞胚胎发生进行植株再生的能力最高的是 malaccensis,其次是 microcarpa、sumatrana 和 breviformis。野生香蕉胚胎发生培养物由体细胞胚、体细胞胚块、原胚和原胚块组成,通过体细胞胚芽萌发和原胚增殖进行增殖。悬浮培养物的维持和增殖是通过亚培养中等大小的细胞聚集体(300 至 1000 微米)来实现的。当接种密度为每 30 mL 培养基 0.3 g 时,培养物的增殖率在 25 d 内达到 7 倍。从 malaccensis 和 microcarpa 悬浮培养物中获得的胚胎培养物在转移到半固体体细胞胚胎发育培养基后能够形成体细胞胚胎,随后在半固体植物再生培养基上发育出嫩芽,转化效率分别为 35% 和 17%。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Induction, growth, and characteristics of embryonic cell suspension culture of wild bananas (Musa acuminata ssp.)

Induction, growth, and characteristics of embryonic cell suspension culture of wild bananas (Musa acuminata ssp.)

Wild bananas and their relatives are potentially utilized for pre-breeding due to their genetic diversity, disease resistance, and tolerance to abiotic stress, and other desirable traits. The embryonic suspension culture of wild bananas provides a means to harness this genetic diversity for banana genetic improvement. This paper elucidates the response of different subspecies M. acuminata (ssp. malaccensis, microcarpa, sumatrana, and breviformis) to the induction, growth, and behavior of suspension cultures and their regeneration into plantlets. Different subspecies exhibit varied responses starting from the embryogenic culture induction stage, culture proliferation, to plantlet formation. The highest competence for plant regeneration through somatic embryogenesis was found in ssp. malaccensis, followed by microcarpa, sumatrana, and breviformis. The wild banana embryogenic culture consists of somatic embryos, somatic embryo masses, proembryos, and proembryonic masses, and it proliferates through somatic embryo budding and proembryo proliferation. Maintenance and proliferation of suspension cultures were achieved through subculturing medium-sized cell aggregates (300 to 1000 µm). With an inoculum density of 0.3 g per 30 mL medium, the culture’s proliferation rate reached seven times within 25 d. Embryogenic cultures from the suspensions of ssp. malaccensis and microcarpa were capable of forming somatic embryos upon transfer to a semi-solid somatic embryo development medium and later developed shoots on a semi-solid plant regeneration medium, with conversion efficiencies of 35% and 17%, respectively.

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来源期刊
CiteScore
5.00
自引率
7.70%
发文量
71
审稿时长
6-12 weeks
期刊介绍: Founded in 1965, In Vitro Cellular & Developmental Biology - Plant is the only journal devoted solely to worldwide coverage of in vitro biology in plants. Its high-caliber original research and reviews make it required reading for anyone who needs comprehensive coverage of the latest developments and state-of-the-art research in plant cell and tissue culture and biotechnology from around the world.
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