鳞翅目:蟹科)的肠道细菌群落受水稻品种的影响

IF 1.7 3区 农林科学 Q2 ENTOMOLOGY
Sheng Lin, Bang Zhang, Qiang Li, Xue-wen Li, Qi-tang Guo, Wen-le Chen, Hui-yu Jiao, Komivi Senyo Akutse, Xiang-zhen Zhu
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引用次数: 0

摘要

为了解日粮对稻纵卷叶螟(Cnaphalocrocis medinalis,RLF)肠道细菌的影响,我们采用培养法比较了从明惠82(RLF1)、永永15(RLF2)和明惠2155(RLF3)三个不同水稻品种田间采集的稻纵卷叶螟幼虫肠道细菌群落的组成和多样性。采用与培养无关的 PCR(V3 可变区)-变性梯度凝胶电泳(PCR-DGGE)方法,并利用 Illumina HiSeq 平台对 16S rRNA 的超变区(V4)进行测序,比较了从明辉 82(RLF1)、永勇 15(RLF2)和明辉 2155(RLF3)三个不同水稻品种田中采集的褐飞虱幼虫肠道细菌群落的组成和多样性。DGGE 结果显示,RLF1、RLF2 和 RLF3 的肠道中共存有 Asaia、Bacillus、Stenotrophomonas、Achromobacter 和 Serratia 等菌属,而 RLF2 和 RLF3 的肠道中则共存有 Cedecea、Burkholderia 和 Acinetobacter 等菌属。然而,只有肠球菌属存在于 RLF1 幼虫的内脏中,泛变形菌属、狼杆菌属和担子菌属存在于 RLF3 幼虫的内脏中。利用 16s RNA 测序共鉴定出 25 个细菌门、48 个类、127 个目、223 个科和 406 个属,其中 35 个属在 RLF1、RLF2 和 RLF3 幼虫内脏中共存,而在 RLF1、RLF2 和 RLF3 中分别存在 83、36 和 141 个独特的属。RLF1、RLF2和RLF3的ACE指数和Shannon指数没有明显差异。主要的细菌类群在门一级为变形菌门、类杆菌门和固着菌门,在属一级为不动杆菌属和狼杆菌属。相对丰度(≥0.01)的属在 RLF1、RLF2 和 RLF3 之间有显著差异。研究结果表明,不同水稻品种对 RLF 中肠道细菌的相对丰度有显著影响,这可以为昆虫肠道细菌与其相关寄主植物之间的关系提供新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Gut bacterial community of Cnaphalocrocis medinalis (Guenée) (Lepidoptera: Crambidae) is driven by rice varieties

To understand the effect of diet on gut bacteria of Cnaphalocrocis medinalis (rice leaf folder, RLF), we compared the composition and diversity of gut bacterial community in C. medinalis larvae collected from three different rice variety fields of Minghui 82 (RLF1), Youngyong 15 (RLF2) and Minghui 2155 (RLF3), using culture-independent PCR (V3 variable region)-denaturing gradient gel electrophoresis (PCR-DGGE) methods and hypervariable region (V4) of 16S rRNA sequenced by Illumina HiSeq platform. The DGGE result showed that the bacteria genera Asaia, Bacillus, Stenotrophomonas, Achromobacter and Serratia coexisted in the guts of RLF1, RLF2 and RLF3, while Cedecea, Burkholderia and Acinetobacter coexisted in the guts of RLF2 and RLF3. However, only the genus Enterococcus existed in the guts of RLF1, and Pantoea, Wolbachia and Tumebacillus in RLF3 larvae. A total of 25 bacterial phyla, 48 classes, 127 orders, 223 families, and 406 genera were identified when using 16s RNA sequencing, with 35 genera coexisted in RLF1, RLF2 and RLF3 larval guts, while 83, 36, 141 unique genera existed in RLF1, RLF2 and RLF3, respectively. The indices of ACE and Shannon were not significantly different among RLF1, RLF2 and RLF3. The dominant bacterial taxa were Proteobacteria, Bacteroidetes and Firmicutes at the phylum level, and Acinetobacter and Wolbachia at the genus level. The relative genus abundance for the genera with relative abundance (≥0.01) was significantly different among RLF1, RLF2 and RLF3. The findings indicated that different rice varieties had significant effects on the relative abundance of gut bacteria in RLF, which could provide new insights into the relationship between insect gut bacteria and their associated host plants.

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来源期刊
CiteScore
3.40
自引率
5.30%
发文量
132
审稿时长
6 months
期刊介绍: The Journal of Applied Entomology publishes original articles on current research in applied entomology, including mites and spiders in terrestrial ecosystems. Submit your next manuscript for rapid publication: the average time is currently 6 months from submission to publication. With Journal of Applied Entomology''s dynamic article-by-article publication process, Early View, fully peer-reviewed and type-set articles are published online as soon as they complete, without waiting for full issue compilation.
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