{"title":"利用微流体通道分析精子细胞在高粘度牛顿和非牛顿流体中的运动情况","authors":"Dhiraj B. Puri, Sumit Sunil Kumar, Vadiraj Hemadri, Arnab Banerjee, Siddhartha Tripathi","doi":"10.1007/s10404-024-02707-1","DOIUrl":null,"url":null,"abstract":"<div><p>The migration of sperm cells in a female reproductive tract is responsible for the successful fertilization of the female egg. In this research work, the effect of the surrounding fluids on the motion of sperm cells has been studied using a microfluidic channel. To analyze the motility of sperm, primary motility parameters such as velocity, beat frequency, amplitude, and derived parameters such as linearity, straightness, and wobble have been measured. The results indicate that sperms possess higher progressive motility in non-Newtonian fluids compared to Newtonian fluids in the same viscosity range. The motion of the sperm shows an inverse relationship between the amplitude of the head trajectory and the beat frequency of the flagella. Numerical studies were performed to measure the drag force on these sperm. The trajectories of the flagella, forces acting on sperm, power generated, pulling power, and efficiency of the sperm motion through the fluid medium have been investigated and a relationship between the force and rotation of the flagella has been established. The results show that the flagella also change their shape based on the properties of the surrounding fluid. This study aims to improve our understanding of issues related to infertility diagnosis and help design in-vitro experiments required for sperm separation.</p></div>","PeriodicalId":706,"journal":{"name":"Microfluidics and Nanofluidics","volume":null,"pages":null},"PeriodicalIF":2.3000,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Analysis of sperm cell motion in high viscosity Newtonian and non-Newtonian fluids using a microfluidic channel\",\"authors\":\"Dhiraj B. Puri, Sumit Sunil Kumar, Vadiraj Hemadri, Arnab Banerjee, Siddhartha Tripathi\",\"doi\":\"10.1007/s10404-024-02707-1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The migration of sperm cells in a female reproductive tract is responsible for the successful fertilization of the female egg. In this research work, the effect of the surrounding fluids on the motion of sperm cells has been studied using a microfluidic channel. To analyze the motility of sperm, primary motility parameters such as velocity, beat frequency, amplitude, and derived parameters such as linearity, straightness, and wobble have been measured. The results indicate that sperms possess higher progressive motility in non-Newtonian fluids compared to Newtonian fluids in the same viscosity range. The motion of the sperm shows an inverse relationship between the amplitude of the head trajectory and the beat frequency of the flagella. Numerical studies were performed to measure the drag force on these sperm. The trajectories of the flagella, forces acting on sperm, power generated, pulling power, and efficiency of the sperm motion through the fluid medium have been investigated and a relationship between the force and rotation of the flagella has been established. The results show that the flagella also change their shape based on the properties of the surrounding fluid. This study aims to improve our understanding of issues related to infertility diagnosis and help design in-vitro experiments required for sperm separation.</p></div>\",\"PeriodicalId\":706,\"journal\":{\"name\":\"Microfluidics and Nanofluidics\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2024-02-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microfluidics and Nanofluidics\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s10404-024-02707-1\",\"RegionNum\":4,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"INSTRUMENTS & INSTRUMENTATION\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microfluidics and Nanofluidics","FirstCategoryId":"5","ListUrlMain":"https://link.springer.com/article/10.1007/s10404-024-02707-1","RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INSTRUMENTS & INSTRUMENTATION","Score":null,"Total":0}
Analysis of sperm cell motion in high viscosity Newtonian and non-Newtonian fluids using a microfluidic channel
The migration of sperm cells in a female reproductive tract is responsible for the successful fertilization of the female egg. In this research work, the effect of the surrounding fluids on the motion of sperm cells has been studied using a microfluidic channel. To analyze the motility of sperm, primary motility parameters such as velocity, beat frequency, amplitude, and derived parameters such as linearity, straightness, and wobble have been measured. The results indicate that sperms possess higher progressive motility in non-Newtonian fluids compared to Newtonian fluids in the same viscosity range. The motion of the sperm shows an inverse relationship between the amplitude of the head trajectory and the beat frequency of the flagella. Numerical studies were performed to measure the drag force on these sperm. The trajectories of the flagella, forces acting on sperm, power generated, pulling power, and efficiency of the sperm motion through the fluid medium have been investigated and a relationship between the force and rotation of the flagella has been established. The results show that the flagella also change their shape based on the properties of the surrounding fluid. This study aims to improve our understanding of issues related to infertility diagnosis and help design in-vitro experiments required for sperm separation.
期刊介绍:
Microfluidics and Nanofluidics is an international peer-reviewed journal that aims to publish papers in all aspects of microfluidics, nanofluidics and lab-on-a-chip science and technology. The objectives of the journal are to (1) provide an overview of the current state of the research and development in microfluidics, nanofluidics and lab-on-a-chip devices, (2) improve the fundamental understanding of microfluidic and nanofluidic phenomena, and (3) discuss applications of microfluidics, nanofluidics and lab-on-a-chip devices. Topics covered in this journal include:
1.000 Fundamental principles of micro- and nanoscale phenomena like,
flow, mass transport and reactions
3.000 Theoretical models and numerical simulation with experimental and/or analytical proof
4.000 Novel measurement & characterization technologies
5.000 Devices (actuators and sensors)
6.000 New unit-operations for dedicated microfluidic platforms
7.000 Lab-on-a-Chip applications
8.000 Microfabrication technologies and materials
Please note, Microfluidics and Nanofluidics does not publish manuscripts studying pure microscale heat transfer since there are many journals that cover this field of research (Journal of Heat Transfer, Journal of Heat and Mass Transfer, Journal of Heat and Fluid Flow, etc.).