核因子I A(Nfia)和核因子I B(Nfib)是小鼠出生后神经干细胞自我更新的共同需要。

Stem cells and development Pub Date : 2024-04-01 Epub Date: 2024-03-21 DOI:10.1089/scd.2022.0204
Christine E Campbell, Karstin Webber, Jonathan E Bard, Lee D Chaves, Jason M Osinski, Richard M Gronostajski
{"title":"核因子I A(Nfia)和核因子I B(Nfib)是小鼠出生后神经干细胞自我更新的共同需要。","authors":"Christine E Campbell, Karstin Webber, Jonathan E Bard, Lee D Chaves, Jason M Osinski, Richard M Gronostajski","doi":"10.1089/scd.2022.0204","DOIUrl":null,"url":null,"abstract":"<p><p>Mouse postnatal neural stem cells (pNSCs) can be expanded in vitro in the presence of epidermal growth factor and fibroblast growth factor 2 and upon removal of these factors cease proliferation and generate neurons, astrocytes, and oligodendrocytes. The genetic requirements for self-renewal and lineage-commitment of pNSCs are incompletely understood. In this study, we show that the transcription factors NFIA and NFIB, previously shown individually, to be essential for the normal commitment of pNSCs to the astrocytic lineage in vivo, are jointly required for normal self-renewal of pNSCs in vitro and in vivo. Using conditional knockout alleles of <i>Nfia</i> and <i>Nfib</i>, we show that the simultaneous loss of these two genes under self-renewal conditions in vitro reduces the expression of the proliferation markers PCNA and Ki67, eliminates clonogenicity of the cells, reduces the number of cells in S phase, and induces aberrant differentiation primarily into the neuroblast lineage. This phenotype requires the loss of both genes and is not seen upon loss of <i>Nfia</i> or <i>Nfib</i> alone, nor with combined loss of <i>Nfia</i> and <i>Nfix</i> or <i>Nfib</i> and <i>Nfix</i>. These data demonstrate a unique combined requirement for both <i>Nfia</i> and <i>Nfib</i> for pNSC self-renewal.</p>","PeriodicalId":94214,"journal":{"name":"Stem cells and development","volume":" ","pages":"153-167"},"PeriodicalIF":0.0000,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Nuclear Factor I A and Nuclear Factor I B Are Jointly Required for Mouse Postnatal Neural Stem Cell Self-Renewal.\",\"authors\":\"Christine E Campbell, Karstin Webber, Jonathan E Bard, Lee D Chaves, Jason M Osinski, Richard M Gronostajski\",\"doi\":\"10.1089/scd.2022.0204\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Mouse postnatal neural stem cells (pNSCs) can be expanded in vitro in the presence of epidermal growth factor and fibroblast growth factor 2 and upon removal of these factors cease proliferation and generate neurons, astrocytes, and oligodendrocytes. The genetic requirements for self-renewal and lineage-commitment of pNSCs are incompletely understood. In this study, we show that the transcription factors NFIA and NFIB, previously shown individually, to be essential for the normal commitment of pNSCs to the astrocytic lineage in vivo, are jointly required for normal self-renewal of pNSCs in vitro and in vivo. Using conditional knockout alleles of <i>Nfia</i> and <i>Nfib</i>, we show that the simultaneous loss of these two genes under self-renewal conditions in vitro reduces the expression of the proliferation markers PCNA and Ki67, eliminates clonogenicity of the cells, reduces the number of cells in S phase, and induces aberrant differentiation primarily into the neuroblast lineage. This phenotype requires the loss of both genes and is not seen upon loss of <i>Nfia</i> or <i>Nfib</i> alone, nor with combined loss of <i>Nfia</i> and <i>Nfix</i> or <i>Nfib</i> and <i>Nfix</i>. These data demonstrate a unique combined requirement for both <i>Nfia</i> and <i>Nfib</i> for pNSC self-renewal.</p>\",\"PeriodicalId\":94214,\"journal\":{\"name\":\"Stem cells and development\",\"volume\":\" \",\"pages\":\"153-167\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Stem cells and development\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1089/scd.2022.0204\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/3/21 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Stem cells and development","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1089/scd.2022.0204","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/3/21 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

小鼠出生后神经干细胞(pNSCs)可在EGF和FGF2存在下体外扩增,去除这些因子后停止增殖并生成神经元、星形胶质细胞和少突胶质细胞。目前对 pNSCs 自我更新和品系定向的遗传要求尚不完全清楚。在这里,我们发现转录因子 NFIA 和 NFIB 是 pNSCs 在体外和体内正常自我更新所必需的转录因子。通过条件性敲除 Nfia 和 Nfib 的等位基因,我们发现在体外自我更新条件下同时缺失这两个基因会降低增殖标记 PCNA 和 Ki67 的表达,消除细胞的克隆性,减少处于 S 期的细胞数量,并诱导主要向神经母细胞系的异常分化。这种表型需要同时缺失这两个基因,而单独缺失 Nfia 或 Nfib 以及联合缺失 Nfia 和 Nfix 或 Nfib 和 Nfix 都不会出现这种表型。这些数据证明了 pNSC 自我更新对 Nfia 和 Nfib 的独特联合要求。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Nuclear Factor I A and Nuclear Factor I B Are Jointly Required for Mouse Postnatal Neural Stem Cell Self-Renewal.

Mouse postnatal neural stem cells (pNSCs) can be expanded in vitro in the presence of epidermal growth factor and fibroblast growth factor 2 and upon removal of these factors cease proliferation and generate neurons, astrocytes, and oligodendrocytes. The genetic requirements for self-renewal and lineage-commitment of pNSCs are incompletely understood. In this study, we show that the transcription factors NFIA and NFIB, previously shown individually, to be essential for the normal commitment of pNSCs to the astrocytic lineage in vivo, are jointly required for normal self-renewal of pNSCs in vitro and in vivo. Using conditional knockout alleles of Nfia and Nfib, we show that the simultaneous loss of these two genes under self-renewal conditions in vitro reduces the expression of the proliferation markers PCNA and Ki67, eliminates clonogenicity of the cells, reduces the number of cells in S phase, and induces aberrant differentiation primarily into the neuroblast lineage. This phenotype requires the loss of both genes and is not seen upon loss of Nfia or Nfib alone, nor with combined loss of Nfia and Nfix or Nfib and Nfix. These data demonstrate a unique combined requirement for both Nfia and Nfib for pNSC self-renewal.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信