{"title":"普通芽孢杆菌与精油提取物结合使用，可减轻番茄纹枯病和细菌斑点病","authors":"Imane Es-sahm , Siham Esserti , Jamila Dich , Amal Smaili , Lalla Aicha Rifai , Lydia Faize , Tayeb Koussa , Jean Stéphane Venisse , Yousra Benyahia , Naima Sawadi , Halima Rabib , Wadi Badri , Mohamed Faize","doi":"10.1016/j.rhisph.2024.100865","DOIUrl":null,"url":null,"abstract":"<div><p>This study was conducted to evaluate the effect of growth promoting rhizobacteria (PGPR) alone and in combination with an essential oil (EO) extracted from <em>Tetraclinis articulata</em> (Vahl) Masters in protecting tomato plants against verticillium wilt and bacterial speck diseases. Three strains, identified as <em>Bacillus toyonensis EI</em>, <em>Bacillus thuringiensi</em>s <em>ES</em> and <em>Bacillus thuringiensi</em>s <em>RA,</em> possess <em>in vitro</em> PGPR activity such as the production of indole acetic acid and ammonia, and the solubilization of phosphates. <em>In planta</em>, they significantly increased the stem length, number of leaflets, leaf area, and root biomass. EO and the three strains, alone or in combination, were able to enhance photosynthetic parameters such as net photosynthesis and transpiration rate. They also significantly reduced leaf alteration (LA) index, stunting index (SI) and browning index (BI) caused by <em>Verticillium dahliae</em> kleb. In tomato. Reduction of LAI ranged from 25% to 37%, and it enhanced to more than 60% when plants were treated with EO combined with <em>B. thuringiensi</em>s <em>SE</em> or with <em>B. toyonensis EI</em>, indicating a synergistic effect. Moreover, EO as well as the three <em>Bacillus</em> strains were also able to enhance tomato resistance against bacterial speck disease caused by <em>Pseudomonas syringae</em> pv. <em>tomato</em>. The average number of lesions was significantly reduced by the application of EO and the three strains, reaching 94% with combination of EO with <em>B. thuringiensi</em>s <em>RA</em>. Bacterial population analyses showed that plants with reduced symptoms exhibited significantly lower bacterial concentrations. In tomato plants inoculated with <em>V. dahliae</em> results showed that EO as well as three strains were able to enhance H<sub>2</sub>O<sub>2</sub> accumulation, at the late stage of infection, which might lead to reduce disease. However, within the pathosystem tomato/<em>P. syringe</em> pv. <em>tomato,</em> H<sub>2</sub>O<sub>2</sub> accumulation was highly enhanced by the pathogen and inhibited after combined treatment.</p></div>","PeriodicalId":3,"journal":{"name":"ACS Applied Electronic Materials","volume":null,"pages":null},"PeriodicalIF":4.3000,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Common Bacillus mitigate tomato verticillium wilt and bacterial specks when combined with an essential oil extract\",\"authors\":\"Imane Es-sahm , Siham Esserti , Jamila Dich , Amal Smaili , Lalla Aicha Rifai , Lydia Faize , Tayeb Koussa , Jean Stéphane Venisse , Yousra Benyahia , Naima Sawadi , Halima Rabib , Wadi Badri , Mohamed Faize\",\"doi\":\"10.1016/j.rhisph.2024.100865\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>This study was conducted to evaluate the effect of growth promoting rhizobacteria (PGPR) alone and in combination with an essential oil (EO) extracted from <em>Tetraclinis articulata</em> (Vahl) Masters in protecting tomato plants against verticillium wilt and bacterial speck diseases. Three strains, identified as <em>Bacillus toyonensis EI</em>, <em>Bacillus thuringiensi</em>s <em>ES</em> and <em>Bacillus thuringiensi</em>s <em>RA,</em> possess <em>in vitro</em> PGPR activity such as the production of indole acetic acid and ammonia, and the solubilization of phosphates. <em>In planta</em>, they significantly increased the stem length, number of leaflets, leaf area, and root biomass. EO and the three strains, alone or in combination, were able to enhance photosynthetic parameters such as net photosynthesis and transpiration rate. They also significantly reduced leaf alteration (LA) index, stunting index (SI) and browning index (BI) caused by <em>Verticillium dahliae</em> kleb. In tomato. Reduction of LAI ranged from 25% to 37%, and it enhanced to more than 60% when plants were treated with EO combined with <em>B. thuringiensi</em>s <em>SE</em> or with <em>B. toyonensis EI</em>, indicating a synergistic effect. Moreover, EO as well as the three <em>Bacillus</em> strains were also able to enhance tomato resistance against bacterial speck disease caused by <em>Pseudomonas syringae</em> pv. <em>tomato</em>. The average number of lesions was significantly reduced by the application of EO and the three strains, reaching 94% with combination of EO with <em>B. thuringiensi</em>s <em>RA</em>. Bacterial population analyses showed that plants with reduced symptoms exhibited significantly lower bacterial concentrations. In tomato plants inoculated with <em>V. dahliae</em> results showed that EO as well as three strains were able to enhance H<sub>2</sub>O<sub>2</sub> accumulation, at the late stage of infection, which might lead to reduce disease. However, within the pathosystem tomato/<em>P. syringe</em> pv. <em>tomato,</em> H<sub>2</sub>O<sub>2</sub> accumulation was highly enhanced by the pathogen and inhibited after combined treatment.</p></div>\",\"PeriodicalId\":3,\"journal\":{\"name\":\"ACS Applied Electronic Materials\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.3000,\"publicationDate\":\"2024-02-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Applied Electronic Materials\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2452219824000181\",\"RegionNum\":3,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENGINEERING, ELECTRICAL & ELECTRONIC\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Electronic Materials","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2452219824000181","RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, ELECTRICAL & ELECTRONIC","Score":null,"Total":0}
引用次数: 0
摘要
本研究旨在评估生长促进根瘤菌（PGPR）单独使用或与从 Tetraclinis articulata (Vahl) Masters 中提取的精油（EO）结合使用对番茄植株抗眩晕枯萎病和细菌性斑点病的效果。三种菌株被鉴定为苏云金芽孢杆菌 EI、苏云金芽孢杆菌 ES 和苏云金芽孢杆菌 RA，具有体外 PGPR 活性，如产生吲哚乙酸和氨，以及溶解磷酸盐。在植物体内，它们能明显增加茎长、小叶数、叶面积和根的生物量。环氧乙烷和这三种菌株单独或混合使用都能提高光合作用参数，如净光合作用和蒸腾速率。它们还能明显降低由大丽轮枝菌（Verticillium dahliae kleb.）引起的叶片变化指数（LA）、发育不良指数（SI）和褐变指数（BI）。在番茄中。当 EO 与苏云金芽孢杆菌 SE 或丰产芽孢杆菌 EI 一起处理植物时，LAI 的降低幅度在 25% 到 37% 之间，而当 EO 与苏云金芽孢杆菌 SE 或丰产芽孢杆菌 EI 一起处理植物时，LAI 的降低幅度提高到 60% 以上，这表明存在协同效应。此外，EO 和三种芽孢杆菌菌株还能增强番茄对由 Pseudomonas syringae pv. tomato 引起的细菌性斑点病的抗性。施用环氧乙烷和三种菌株后，病斑的平均数量明显减少，环氧乙烷与苏云金芽孢杆菌 RA 结合施用时，病斑的平均数量减少了 94%。细菌数量分析表明，症状减轻的植株细菌浓度明显较低。在接种了大丽花病毒的番茄植株上，结果表明，在感染后期，EO 和三种菌株都能提高 H2O2 的积累，这可能会减少病害。然而，在番茄/P. syringe pv. tomato 的病原系统中，病原菌高度增强了 H2O2 的积累，而联合处理则抑制了 H2O2 的积累。
Common Bacillus mitigate tomato verticillium wilt and bacterial specks when combined with an essential oil extract
This study was conducted to evaluate the effect of growth promoting rhizobacteria (PGPR) alone and in combination with an essential oil (EO) extracted from Tetraclinis articulata (Vahl) Masters in protecting tomato plants against verticillium wilt and bacterial speck diseases. Three strains, identified as Bacillus toyonensis EI, Bacillus thuringiensis ES and Bacillus thuringiensis RA, possess in vitro PGPR activity such as the production of indole acetic acid and ammonia, and the solubilization of phosphates. In planta, they significantly increased the stem length, number of leaflets, leaf area, and root biomass. EO and the three strains, alone or in combination, were able to enhance photosynthetic parameters such as net photosynthesis and transpiration rate. They also significantly reduced leaf alteration (LA) index, stunting index (SI) and browning index (BI) caused by Verticillium dahliae kleb. In tomato. Reduction of LAI ranged from 25% to 37%, and it enhanced to more than 60% when plants were treated with EO combined with B. thuringiensis SE or with B. toyonensis EI, indicating a synergistic effect. Moreover, EO as well as the three Bacillus strains were also able to enhance tomato resistance against bacterial speck disease caused by Pseudomonas syringae pv. tomato. The average number of lesions was significantly reduced by the application of EO and the three strains, reaching 94% with combination of EO with B. thuringiensis RA. Bacterial population analyses showed that plants with reduced symptoms exhibited significantly lower bacterial concentrations. In tomato plants inoculated with V. dahliae results showed that EO as well as three strains were able to enhance H2O2 accumulation, at the late stage of infection, which might lead to reduce disease. However, within the pathosystem tomato/P. syringe pv. tomato, H2O2 accumulation was highly enhanced by the pathogen and inhibited after combined treatment.
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