Doreen Enyang, Mubo A Sonibare, Armelle D Tchamgoue, Lauve R Y Tchokouaha, Fanta S Yadang, Gael N Nfor, Christelle W Kom, Patrick D H Betote, Cedric F Tchinda, Steveng S K Tiogo, Gabriel A Agbor
{"title":"瓜蒌茎叶水乙醇提取物对Wistar大鼠抗逆转录病毒疗法引起的肝毒性和血脂异常的保护和改善作用","authors":"Doreen Enyang, Mubo A Sonibare, Armelle D Tchamgoue, Lauve R Y Tchokouaha, Fanta S Yadang, Gael N Nfor, Christelle W Kom, Patrick D H Betote, Cedric F Tchinda, Steveng S K Tiogo, Gabriel A Agbor","doi":"10.1155/2024/5811080","DOIUrl":null,"url":null,"abstract":"<p><p>Antiretroviral therapy (ART) has revolutionized the lives of people living with HIV/AIDS by overall improving their quality of life and increasing life expectancy. However, ART-associated hepatotoxicity and metabolic disorders in HIV/AIDS patients are growing concerns to clinicians, especially due to the long-term use of the drugs. This study reported on the phytochemical and pharmacological profile of hydroethanolic extracts of <i>Piper nigrum</i> stem (PNS) and evaluated its protective effect against tenofovir/lamivudine/efavirenz (TLE)-induced hepatotoxicity and dyslipidemia in Wistar rats. Cytotoxic, antioxidant, and anti-inflammatory assays were performed on PNS. Thirty-six rats divided into 6 groups of 6 animals/group were administered: distilled water, 17 mg/kg TLE, 17 mg/kg TLE and 100 mg/kg silymarin, 17 mg/kg TLE, and <i>Piper</i> extract (200 mg/kg, 400 mg/kg, or 800 mg/kg) orally for 28 days. The body weight of animals was recorded every 7 days. On Day 29, the rats were sacrificed, and blood samples were collected for hematological and biochemical tests. Portions of the liver and kidneys were collected for histological evaluation, while liver homogenates were prepared from the rest to measure antioxidant enzymes. PNS possessed in vitro cytotoxic, antioxidant, and anti-inflammatory activities. A significant decrease (<i>p</i> < 0.05) in the body weight of rats treated with PNS was observed. A significant high platelet count (<i>p</i> < 0.05) was observed in the PNS800 mg/kg group. A considerable decrease in alkaline phosphatase and triglycerides was observed in the silymarin and PNS group compared to the TLE-only group. The findings also show a significant increase in catalase and glutathione in the TLE-only group compared to the normal group, while SOD decreased. Histological observations revealed normal hepatic and renal tissues in the silymarin, and PNS-treated groups compared to the normal control, while leucocyte infiltration was observed in the TLE-only group. These results suggest that PNS extract possessed antioxidant activity that alleviated TLE-induced toxicity. 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引用次数: 0
摘要
抗逆转录病毒疗法(ART)全面改善了艾滋病毒/艾滋病感染者的生活质量,延长了他们的预期寿命,从而彻底改变了他们的生活。然而,抗逆转录病毒疗法相关的肝脏毒性和艾滋病毒/艾滋病患者的代谢紊乱日益引起临床医生的关注,尤其是由于长期使用该药物。本研究报告了黑胡椒茎(PNS)水乙醇提取物的植物化学和药理学特征,并评估了其对替诺福韦/拉米夫定/依维仑(TLE)诱导的 Wistar 大鼠肝毒性和血脂异常的保护作用。对 PNS 进行了细胞毒性、抗氧化和抗炎试验。将 36 只大鼠分为 6 组,每组 6 只,分别口服蒸馏水、17 毫克/千克 TLE、17 毫克/千克 TLE 和 100 毫克/千克水飞蓟素、17 毫克/千克 TLE 和胡椒提取物(200 毫克/千克、400 毫克/千克或 800 毫克/千克),连续 28 天。每 7 天记录一次动物体重。第 29 天,大鼠被处死,并采集血液样本进行血液和生化测试。收集部分肝脏和肾脏进行组织学评估,其余肝脏匀浆用于测量抗氧化酶。PNS 具有体外细胞毒性、抗氧化和抗炎活性。经 PNS 处理的大鼠体重明显下降(p < 0.05)。在 PNS800 毫克/千克组中观察到血小板计数明显偏高(p < 0.05)。与单纯 TLE 组相比,水飞蓟素和 PNS 组的碱性磷酸酶和甘油三酯显著下降。研究结果还显示,与正常组相比,仅患狼疮组的过氧化氢酶和谷胱甘肽明显增加,而 SOD 则有所下降。组织学观察显示,与正常对照组相比,水飞蓟素和 PNS 处理组的肝脏和肾脏组织正常,而只用 TLE 组则观察到白细胞浸润。这些结果表明,PNS 提取物具有抗氧化活性,可减轻 TLE 诱导的毒性。有必要开展进一步研究,以了解 ART 与 PNS 之间的药代动力学相互作用。
Protective and Ameliorative Effects of Hydroethanolic Extract of Piper nigrum (L.) Stem against Antiretroviral Therapy-Induced Hepatotoxicity and Dyslipidemia in Wistar Rats.
Antiretroviral therapy (ART) has revolutionized the lives of people living with HIV/AIDS by overall improving their quality of life and increasing life expectancy. However, ART-associated hepatotoxicity and metabolic disorders in HIV/AIDS patients are growing concerns to clinicians, especially due to the long-term use of the drugs. This study reported on the phytochemical and pharmacological profile of hydroethanolic extracts of Piper nigrum stem (PNS) and evaluated its protective effect against tenofovir/lamivudine/efavirenz (TLE)-induced hepatotoxicity and dyslipidemia in Wistar rats. Cytotoxic, antioxidant, and anti-inflammatory assays were performed on PNS. Thirty-six rats divided into 6 groups of 6 animals/group were administered: distilled water, 17 mg/kg TLE, 17 mg/kg TLE and 100 mg/kg silymarin, 17 mg/kg TLE, and Piper extract (200 mg/kg, 400 mg/kg, or 800 mg/kg) orally for 28 days. The body weight of animals was recorded every 7 days. On Day 29, the rats were sacrificed, and blood samples were collected for hematological and biochemical tests. Portions of the liver and kidneys were collected for histological evaluation, while liver homogenates were prepared from the rest to measure antioxidant enzymes. PNS possessed in vitro cytotoxic, antioxidant, and anti-inflammatory activities. A significant decrease (p < 0.05) in the body weight of rats treated with PNS was observed. A significant high platelet count (p < 0.05) was observed in the PNS800 mg/kg group. A considerable decrease in alkaline phosphatase and triglycerides was observed in the silymarin and PNS group compared to the TLE-only group. The findings also show a significant increase in catalase and glutathione in the TLE-only group compared to the normal group, while SOD decreased. Histological observations revealed normal hepatic and renal tissues in the silymarin, and PNS-treated groups compared to the normal control, while leucocyte infiltration was observed in the TLE-only group. These results suggest that PNS extract possessed antioxidant activity that alleviated TLE-induced toxicity. Further studies are necessary to understand the pharmacokinetic interactions between ART and PNS.
期刊介绍:
Journal of Toxicology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of toxicological sciences. The journal will consider articles looking at the structure, function, and mechanism of agents that are toxic to humans and/or animals, as well as toxicological medicine, risk assessment, safety evaluation, and environmental health.