在饲料中添加唾液酸利吉拉特氏菌 PS21603 可优化断奶仔猪的肠道形态和肠道微生物群组成。

IF 3 4区医学 Q2 MICROBIOLOGY

Beneficial microbes Pub Date : 2024-01-31 DOI:10.1163/18762891-bja00001

I Cuevas-Gómez, J de Andrés, N Cardenas, I Espinosa-Martos, E Jiménez

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引用次数: 0

摘要

仔猪断奶后腹泻仍然是造成养猪生产者经济损失的一个重要原因。在饲料中添加益生菌是抗生素的替代品之一，可用于减少此类胃肠道疾病的影响。本研究的目的是评估补充唾液酸左旋乳杆菌 PS21603 对断奶仔猪肠道结构和肠道微生物群组成的影响。在一项为期 28 天的研究中，使用 384 头断奶仔猪（28 ± 2 日龄，7.5 ± 1.5 千克）进行了唾液酸ligilactacillus PS21603 的安全性和耐受性评估，这些仔猪分为三个处理组：T1:T1：基础日粮 + L. salivarius PS21603 109 cfu/天；T2：基础日粮 + L. salivarius PS21603 107 cfu/天；T3：基础日粮（对照组）：基础日粮（对照组）。在本研究中，每个处理组随机选取 16 头仔猪，在研究的第 0 天（断奶）和第 28 天采集粪便样本。研究结束时，对每个处理组的三头雄性和三头雌性仔猪实施安乐死。尸体解剖后测量肠道形态测量值。粪便中大肠杆菌的数量通过培养技术进行评估，粪便微生物群的组成通过高通量测序技术进行评估。对所有数据进行分析，并在处理组之间进行比较。与第 28 天的 T3 组相比，添加唾液球菌 PS21603 可增加 T1 组仔猪的肠道长度和 T2 组仔猪的绒毛高度与隐窝比率（P < 0.05）。根据香农多样性指数（Shannon Diversity Index），与第 0 天相比，第 28 天的微生物群多样性有所增加，不同处理之间没有观察到显著差异。在不同采样时间点之间，观察到细菌在门、科、属水平上相对丰度的主要变化。然而，在第 28 天，T1 和 T2 的仔猪粪便中的大肠杆菌数量低于 T3（P < 0.05）。此外，从研究开始到结束，补充唾液酸梭菌 PS21603 能通过更优化的组成调节肠道微生物群，减少 T1 仔猪的埃希氏菌，增加双歧杆菌的相对丰度（P < 0.05）。因此，L. salivarius PS21603 菌株已显示出益生菌特性，可作为养猪业的饲料添加剂，配合良好的卫生和农场管理措施，用于预防和/或治疗仔猪断奶后腹泻。

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Feed supplementation with Ligilactobacillus salivarius PS21603 optimises intestinal morphology and gut microbiota composition in weaned piglets.

Post-weaning diarrhoea in piglets remains an important cause of economic losses for swine producers. Feed supplementation with probiotics is one of the alternatives to antibiotics used to reduce the impact of such gastrointestinal disease. The aim of the present study was to evaluate the effect of Ligilactobacillus salivarius PS21603 supplementation on the intestinal structure and the gut microbiota composition of weaned piglets. Safety and tolerance of L. salivarius PS21603 were previously evaluated in a 28-days study using 384 weaned piglets (28 ± 2 days old and 7.5 ± 1.5 kg) divided in three treatment groups: T1: Basal diet + L. salivarius PS21603 109 cfu/day, T2: Basal diet + L. salivarius PS21603 107 cfu/day, and T3: Basal diet (control group). For the present study, 16 piglets per treatment group were randomly selected and faecal samples were collected on day 0 (weaning) and 28 of study. At the end of study, three males and three females per treatment were euthanised. Intestinal morphometric values were measured after necropsy. Faecal counts of Escherichia coli were evaluated by culture techniques, and faecal microbiota composition was assessed by high-throughput sequencing. All data were analysed and compared between treatment groups. Supplementation with L. salivarius PS21603 caused an increase in the intestine length of piglets from T1 and in the villous height:crypt ratio of piglets from T2 (P < 0.05) compared to T3 on day 28. According to the Shannon Diversity Index, microbiota diversity increased on day 28 compared to day 0, with no significant differences observed between treatments. The main changes in the relative abundance of bacteria at the phylum, family, and genus levels were observed between different sampling time points. However, piglets from T1 and T2 had lower faecal E. coli counts than T3 on day 28 (P < 0.05). Moreover, supplementation with L. salivarius PS21603 modulated gut microbiota through a more optimal composition, reducing Escherichia and increasing Bifidobacterium relative abundance in piglets from T1 (P < 0.05) from the beginning to the end of the study. Therefore, the strain L. salivarius PS21603 has shown probiotic properties to be used as feed additive in the pig industry, along with good hygiene and farm management practices, for the prevention and/or treatment of post-weaning diarrhoea in piglets.

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来源期刊

Beneficial microbes MICROBIOLOGY-NUTRITION & DIETETICS

CiteScore

7.90

自引率

1.90%

发文量

审稿时长

>12 weeks

期刊介绍： Beneficial Microbes is a peer-reviewed scientific journal with a specific area of focus: the promotion of the science of microbes beneficial to the health and wellbeing of man and animal. The journal contains original research papers and critical reviews in all areas dealing with beneficial microbes in both the small and large intestine, together with opinions, a calendar of forthcoming beneficial microbes-related events and book reviews. The journal takes a multidisciplinary approach and focuses on a broad spectrum of issues, including safety aspects of pro- & prebiotics, regulatory aspects, mechanisms of action, health benefits for the host, optimal production processes, screening methods, (meta)genomics, proteomics and metabolomics, host and bacterial physiology, application, and role in health and disease in man and animal. Beneficial Microbes is intended to serve the needs of researchers and professionals from the scientific community and industry, as well as those of policy makers and regulators. The journal will have five major sections: * Food, nutrition and health * Animal nutrition * Processing and application * Regulatory & safety aspects * Medical & health applications In these sections, topics dealt with by Beneficial Microbes include: * Worldwide safety and regulatory issues * Human and animal nutrition and health effects * Latest discoveries in mechanistic studies and screening methods to unravel mode of action * Host physiology related to allergy, inflammation, obesity, etc. * Trends in application of (meta)genomics, proteomics and metabolomics * New developments in how processing optimizes pro- & prebiotics for application * Bacterial physiology related to health benefits