IL6R和IL10基因多态性与伴有或不伴有急性前葡萄膜炎的强直性脊柱炎易感性的关系

IF 2.6 4区医学 Q2 OPHTHALMOLOGY

Ocular Immunology and Inflammation Pub Date : 2025-01-01 Epub Date: 2024-02-12 DOI:10.1080/09273948.2024.2309279

Li Lin, Fuzhen Li, Haixin Fang, Pengyi Zhou, Jiankang Shan, Kunpeng Xie, Bo Jin, Haiyan Zhu, Xuemin Jin, Liping Du, Peizeng Yang

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引用次数: 0

摘要

研究背景本研究旨在探讨白细胞介素-6受体（IL6R）和白细胞介素-10（IL10）基因上的10个候选单核苷酸多态性（SNPs）与伴有或不伴有急性前葡萄膜炎（AAU）的强直性脊柱炎（AS）患者之间的关联：本研究采用病例对照方法，对354例强直性脊柱炎和AAU患者、377例强直性脊柱炎而无AAU患者以及918例健康对照者进行了研究。采用iPLEX Gold基因分型方法对IL10和IL6R基因的10个SNPs进行了基因分型。采用卡方检验对比了病例和健康人的等位基因和基因型频率。使用实时 PCR 检测了不同 IL10 基因型的 IL10 mRNA 水平：结果：发现了两个与AS伴AAU相关的基因位点：IL10//rs3790622（OR=0.664；95%CI=0.503-0.878；Pc=0.038）；IL10//rs3021094（OR=1.365；95%CI=1.110-1.679；Pc=0.032）。位于 IL10 和 IL6R 上的其他 8 个基因位点与疾病无显著关联。此外，功能实验表明，IL10 的 mRNA 表达与各种基因型之间没有相关性：我们的研究表明，IL10 基因是中国人患强直性脊柱炎和 AAU 的易感基因。

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Associations of IL6R and IL10 Gene Polymorphisms with Susceptibility to Ankylosing Spondylitis with or without Acute Anterior Uveitis.

Background: This research aims to explore the associations between ten candidate single nucleotide polymorphisms (SNPs) on Interleukin-6 receptor (IL6R) and Interleukin-10 (IL10) genes and ankylosing spondylitis (AS) patients with or without acute anterior uveitis (AAU).

Methods: This study involved a case-control approach that examined 354 cases with AS and AAU, 377 AS cases without AAU, and 918 healthy controls. Genotyping of ten SNPs of IL10 and IL6R genes was performed using iPLEX Gold genotyping method. The allele and genotype frequencies of cases and healthy individuals were contrasted using the chi-square test. The IL10 mRNA level in various IL10 genotypes was tested using real-time PCR.

Results: Two loci associated with AS with AAU were identified: IL10//rs3790622 (OR = 0.664; 95%CI = 0.503-0.878; Pc = 0.038); IL10//rs3021094 (OR = 1.365; 95%CI = 1.110-1.679; Pc = 0.032). The other eight loci located on IL10 and IL6R did not show significant associations with the diseases. Additionally, as shown by functional experiments, there was no correlation between the mRNA expression of IL10 and various genotypes.

Conclusion: Our study suggests that the IL10 gene contributes to the susceptibility of the Chinese population to AS with AAU.

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来源期刊

Ocular Immunology and Inflammation 医学-眼科学

CiteScore

6.20

自引率

15.20%

发文量

285

审稿时长

6-12 weeks

期刊介绍： Ocular Immunology & Inflammation ranks 18 out of 59 in the Ophthalmology Category.Ocular Immunology and Inflammation is a peer-reviewed, scientific publication that welcomes the submission of original, previously unpublished manuscripts directed to ophthalmologists and vision scientists. Published bimonthly, the journal provides an international medium for basic and clinical research reports on the ocular inflammatory response and its control by the immune system. The journal publishes original research papers, case reports, reviews, letters to the editor, meeting abstracts, and invited editorials.