Dion Garrett, Graham Teakle, Rosemary Collier, James R. Bell, Sergio Cerezo-Medina, Ramiro Morales-Hojas
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引用次数: 0
摘要
蚜虫基因组资源有助于研究复杂的生活史特征,并提供有关媒介生物学、宿主适应性和物种变异的信息。醋栗莴苣蚜(Nasonovia ribisnigri (Hemiptera: Aphididae) (Mosley))是室外莴苣(Lactuca sativa (Asterales: Asteraceae) (Linnaeus) )的一种世界性害虫。直到最近,使用抗性栽培品种仍是防治 N. ribisnigri 的有效方法。20 世纪 80 年代引入的一种抗性栽培品种含有单一基因(Nr-locus),能完全抵抗食害。在莴苣中过度依赖这种 Nr-locus,导致 N. ribisnigri 能够打破抗性机制,2003 年首次报告了这种情况。我们的工作试图了解哪些候选基因与这种抗性破坏机制有关。我们展示了两个全新的 N. ribisnigri 基因组草案,分别对应无毒(Nr-locus 易感)和有毒(Nr-locus 耐药)生物型。通过对 RNA 序列(RNA-seq)数据进行转录组分析,研究了两种 N. ribisnigri 生物型的基因表达变化,以了解莴苣对 Nr-locus 的抗性的潜在机制。无抗性生物型和有抗性生物型的基因组组装草案的完整率分别为 94.2% 和 91.4%。在 18,872 个差异表达基因中,在 N. ribisnigri 中发现了一个基因/位点,该基因/位点是两个抗性突破生物型共有的。实时定量反转录 PCR(qRT-PCR)实验对该基因座进行了进一步探索和验证,并预测了其在细胞质和细胞核中的定位。这是首次有研究证明,单个基因/位点可能是 N. ribisnigri 在莴苣宿主体内克服 Nr-位点抗性能力的原因。
Genome assembly and transcriptomic analysis to elucidate the ability of Nasonovia ribisnigri to break host plant resistance
Aphid genomic resources enable the study of complex life history traits and provide information on vector biology, host adaption and speciation. The currant–lettuce aphid (Nasonovia ribisnigri(Hemiptera: Aphididae) (Mosley)) is a cosmopolitan pest of outdoor lettuce (Lactuca sativa (Asterales: Asteraceae) (Linnaeus)). Until recently, the use of resistant cultivars was an effective method for managing N. ribisnigri. A resistant cultivar containing a single gene (Nr-locus), introduced in the 1980s, conferred complete resistance to feeding. Overreliance of this Nr-locus in lettuce resulted in N. ribisnigri's ability to break resistance mechanism, with first reports during 2003. Our work attempts to understand which candidate gene(s) are associated with this resistance-breaking mechanism. We present two de novo draft assembles for N. ribisnigri genomes, corresponding to both avirulent (Nr-locus susceptible) and virulent (Nr-locus resistant) biotypes. Changes in gene expression of the two N. ribisnigri biotypes were investigated using transcriptomic analyses of RNA-sequencing (RNA-seq) data to understand the potential mechanisms of resistance to the Nr-locus in lettuce. The draft genome assemblies were 94.2% and 91.4% complete for the avirulent and virulent biotypes, respectively. Out of the 18,872 differentially expressed genes, a single gene/locus was identified in N. ribisnigri that was shared between two resistant-breaking biotypes. This locus was further explored and validated in Real-Time Quantitative Reverse Transcription PCR (qRT-PCR) experiments and has predicted localisations in both the cytoplasm and nucleus. This is the first study to provide evidence that a single gene/locus is likely responsible for the ability of N. ribisnigri to overcome the Nr-locus resistance in the lettuce host.
期刊介绍:
Insect Molecular Biology has been dedicated to providing researchers with the opportunity to publish high quality original research on topics broadly related to insect molecular biology since 1992. IMB is particularly interested in publishing research in insect genomics/genes and proteomics/proteins.
This includes research related to:
• insect gene structure
• control of gene expression
• localisation and function/activity of proteins
• interactions of proteins and ligands/substrates
• effect of mutations on gene/protein function
• evolution of insect genes/genomes, especially where principles relevant to insects in general are established
• molecular population genetics where data are used to identify genes (or regions of genomes) involved in specific adaptations
• gene mapping using molecular tools
• molecular interactions of insects with microorganisms including Wolbachia, symbionts and viruses or other pathogens transmitted by insects
Papers can include large data sets e.g.from micro-array or proteomic experiments or analyses of genome sequences done in silico (subject to the data being placed in the context of hypothesis testing).