2009-2019 年台湾北部临床单核细胞增生李斯特菌分离物的分子特征和毒力特征。

IF 1.9 2区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY
Foodborne pathogens and disease Pub Date : 2024-06-01 Epub Date: 2024-02-12 DOI:10.1089/fpd.2023.0136
Tsui-Ping Liu, Lee-Chung Lin, Shih-Cheng Chang, Yu-Hsiang Ou, Jang-Jih Lu
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引用次数: 0

摘要

单核细胞增生李斯特菌是一种重要的食源性致病菌,可引起严重的侵袭性和非侵袭性疾病,死亡率很高。有关台湾地区单增李斯特菌感染率的信息非常有限。本研究旨在分析 2009 年至 2019 年期间在台湾北部收集到的 176 株人类临床单核细胞增生症菌株的分子流行病学监测和毒力基因分布情况。结果显示,这些分离株属于 4 个血清群(IIa、IIb、IVB 和 IIc),其中大多数分离株属于 IIa 血清群(81/176,46%)和 IIb 血清群(71/176,40.3%)。多焦点序列分型分析显示了 18 个序列类型(ST)和 13 个克隆复合体(CC)。84%的分离株属于 6 个 ST:CC87-ST87(40/176,22.7%)、CC19-ST378(36/176,19.9%)、CC155-ST155(28/176,15.5%)、CC1-ST710(16/176,8.8%)、CC5-ST5(16/176,8.8%)和CC101-ST101(11/176,6.1%)。此外,我们的分析还显示了李斯特菌致病性岛(LIPI)在所有分离株中的分布情况。LIPI-1和LIPI-2存在于所有分离株中,而LIPI-3和LIPI-4只存在于特定的ST和CC中。LIPI-3 存在于 ST、CC1-ST710、CC3-ST3、CC288-ST295 和 CC191-ST1458,而 LIPI-4 存在于 ST、CC87-ST87 和 CC87-ST1459。含有 LIPI-3 和 LIPI-4 的菌株可能具有高病毒性;因此,本研究中收集的 68/176 株分离物(39.1%)可能具有高病毒性。由于单核细胞增生性李斯特菌感染被认为与饮食高度相关,因此对食品中李斯特菌进行分子流行病学监测非常重要;持续监测将为预防食源性疾病提供关键信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Molecular Characteristics and Virulence Profile of Clinical Listeria monocytogenes Isolates in Northern Taiwan, 2009-2019.

Listeria monocytogenes is a critical foodborne pathogen that causes severe invasive and noninvasive diseases and is associated with high mortality. Information on the prevalence of L. monocytogenes infections in Taiwan is very limited. This study aimed to analyze the molecular epidemiological surveillance and virulence gene distribution of 176 human clinical L. monocytogenes isolates collected between 2009 and 2019 in northern Taiwan. Our results showed that the isolates belonged to 4 serogroups (IIa, IIb, IVb, and IIc), with most isolates in serogroups IIa (81/176, 46%) and IIb (71/176, 40.3%). Multilocus sequence typing analysis revealed 18 sequence types (STs) and 13 clonal complexes (CCs). Eighty-four percent of all isolates belonged to six STs: CC87-ST87 (40/176, 22.7%), CC19-ST378 (36/176, 19.9%), CC155-ST155 (28/176, 15.5%), CC1-ST710 (16/176, 8.8%), CC5-ST5 (16/176, 8.8%), and CC101-ST101 (11/176, 6.1%). Furthermore, our analysis showed the distributions of four Listeria pathogenicity islands (LIPI) among all isolates. LIPI-1 and LIPI-2 existed in all isolates, whereas LIPI-3 and LIPI-4 only existed in specific STs and CCs. LIPI-3 existed in the STs, CC1-ST710, CC3-ST3, CC288-ST295, and CC191-ST1458, whereas LIPI-4 could be found in the STs, CC87-ST87 and CC87-ST1459. Strains containing LIPI-3 and LIPI-4 are potentially hypervirulent; thus, 68/176 isolates (39.1%) collected in this study were potentially hypervirulent. Since L. monocytogenes infections are considered highly correlated with diet, molecular epidemiological surveillance of Listeria in food is important; continued surveillance will provide critical information to prevent foodborne diseases.

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来源期刊
Foodborne pathogens and disease
Foodborne pathogens and disease 医学-食品科技
CiteScore
5.30
自引率
3.60%
发文量
80
审稿时长
1 months
期刊介绍: Foodborne Pathogens and Disease is one of the most inclusive scientific publications on the many disciplines that contribute to food safety. Spanning an array of issues from "farm-to-fork," the Journal bridges the gap between science and policy to reduce the burden of foodborne illness worldwide. Foodborne Pathogens and Disease coverage includes: Agroterrorism Safety of organically grown and genetically modified foods Emerging pathogens Emergence of drug resistance Methods and technology for rapid and accurate detection Strategies to destroy or control foodborne pathogens Novel strategies for the prevention and control of plant and animal diseases that impact food safety Biosecurity issues and the implications of new regulatory guidelines Impact of changing lifestyles and consumer demands on food safety.
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