斑马鱼视网膜神经节细胞和初级运动轴突的发育需要一个新基因 drish。

IF 2 3区 生物学 Q2 ANATOMY & MORPHOLOGY
Suman Gurung, Nicole K. Restrepo, Surendra Kumar Anand, Vinoth Sittaramane, Saulius Sumanas
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引用次数: 0

摘要

背景:在神经发生过程中,生长中的轴突必须在复杂的细胞外环境中导航,并建立正确的突触连接,以保证神经回路的正常运作。人们对功能性神经网络的形成机制还只有部分了解:在此,我们分析了新型基因 si:ch73-364h19.1/drish 在斑马鱼胚胎神经和血管发育过程中的作用。结果:我们在这里分析了一个新基因 si:ch73-364h19.1/drish 在斑马鱼胚胎神经和血管发育中的作用。我们发现,在胚胎发育的早期阶段,drish mRNA 在多种细胞类型(包括神经细胞、神经胶质细胞、视网膜祖细胞和血管内皮细胞)中广泛而动态地表达。为了研究Drish在胚胎发育过程中的功能,我们利用CRISPR/Cas9基因组编辑技术产生了drish基因突变体。drish功能缺失突变体幼虫在早期视网膜神经节细胞、视神经和视网膜内核层形成方面表现出缺陷,并出现异位运动轴突分支。此外,drish 突变体成虫表现出视网膜外核层缺陷,并表现出光反应和运动行为缺陷。然而,血管形态和血液循环并未受到明显影响:这些数据共同证明了斑马鱼 drish 在视网膜神经节细胞、视神经和中间神经元发育以及脊髓运动轴突分支中的重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Requirement of a novel gene, drish, in the zebrafish retinal ganglion cell and primary motor axon development

Background

During neurogenesis, growing axons must navigate through the complex extracellular environment and make correct synaptic connections for the proper functioning of neural circuits. The mechanisms underlying the formation of functional neural networks are still only partially understood.

Results

Here we analyzed the role of a novel gene si:ch73-364h19.1/drish in the neural and vascular development of zebrafish embryos. We show that drish mRNA is expressed broadly and dynamically in multiple cell types including neural, glial, retinal progenitor and vascular endothelial cells throughout the early stages of embryonic development. To study Drish function during embryogenesis, we generated drish genetic mutant using CRISPR/Cas9 genome editing. drish loss-of-function mutant larvae displayed defects in early retinal ganglion cell, optic nerve and the retinal inner nuclear layer formation, as well as ectopic motor axon branching. In addition, drish mutant adults exhibited deficient retinal outer nuclear layer and showed defective light response and locomotory behavior. However, vascular patterning and blood circulation were not significantly affected.

Conclusions

Together, these data demonstrate important roles of zebrafish drish in the retinal ganglion cell, optic nerve and interneuron development and in spinal motor axon branching.

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来源期刊
Developmental Dynamics
Developmental Dynamics 生物-发育生物学
CiteScore
5.10
自引率
8.00%
发文量
116
审稿时长
3-8 weeks
期刊介绍: Developmental Dynamics, is an official publication of the American Association for Anatomy. This peer reviewed journal provides an international forum for publishing novel discoveries, using any model system, that advances our understanding of development, morphology, form and function, evolution, disease, stem cells, repair and regeneration.
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