Yael Efrén Díaz-López, Vicenta Cázares-Domínguez, Francisco Arenas-Huertero, Ruth Gutierrez-Aguilar
{"title":"沉默 ETV5 可使 INS-1 (832/13) 细胞系发生间充质向上皮的转化。","authors":"Yael Efrén Díaz-López, Vicenta Cázares-Domínguez, Francisco Arenas-Huertero, Ruth Gutierrez-Aguilar","doi":"10.1055/a-2246-4778","DOIUrl":null,"url":null,"abstract":"<p><p>ETV5 has been described to be involved in the epithelial to mesenchymal transition (EMT) mainly in cancer. It is known that EMT provokes cytoskeleton remodeling, improving cellular migratory, and invasive capabilities. Moreover, overexpression of <i>ETV5</i> has been correlated to cancer development and this gene has been implicated in cell proliferation. However, little is known about the downregulation of <i>ETV5</i> expression in a pancreatic cell line and the inverse mesenchymal to epithelial transition (MET). Therefore, we studied the implications of <i>ETV5</i> silencing over the phenotype of the insulinoma INS-1 (832/13) cell line and described the MET by partial <i>ETV5</i> silencing in the INS-1 (832/13) cell line. The downregulation of <i>ETV5</i> expression was obtained by using <i>ETV5</i> siRNA in the insulinoma rat cell line, INS-1 (832/13). Then, <i>ETV5</i> knockdown provoked a MET phenotype observed by crystal violet staining and verified by immunohistochemistry against E-cadherin. Wound healing assay showed no migration, and F-actin stain revealed rearrangement of actin microfilaments. In addition, <i>TGFβ1</i> and <i>TGFβ3</i> were downregulated in the absence of <i>ETV5</i>. <i>ETV5</i> silencing induces epithelial phenotype by downregulating <i>TGFβ1</i> and <i>TGFβ3</i> in INS-1 (832/13) cell line.</p>","PeriodicalId":12999,"journal":{"name":"Hormone and Metabolic Research","volume":" ","pages":"235-243"},"PeriodicalIF":2.0000,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"ETV5 Silencing Produces Mesenchymal to Epithelial Transition in INS-1 (832/13) Cell Line.\",\"authors\":\"Yael Efrén Díaz-López, Vicenta Cázares-Domínguez, Francisco Arenas-Huertero, Ruth Gutierrez-Aguilar\",\"doi\":\"10.1055/a-2246-4778\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>ETV5 has been described to be involved in the epithelial to mesenchymal transition (EMT) mainly in cancer. It is known that EMT provokes cytoskeleton remodeling, improving cellular migratory, and invasive capabilities. Moreover, overexpression of <i>ETV5</i> has been correlated to cancer development and this gene has been implicated in cell proliferation. However, little is known about the downregulation of <i>ETV5</i> expression in a pancreatic cell line and the inverse mesenchymal to epithelial transition (MET). Therefore, we studied the implications of <i>ETV5</i> silencing over the phenotype of the insulinoma INS-1 (832/13) cell line and described the MET by partial <i>ETV5</i> silencing in the INS-1 (832/13) cell line. The downregulation of <i>ETV5</i> expression was obtained by using <i>ETV5</i> siRNA in the insulinoma rat cell line, INS-1 (832/13). Then, <i>ETV5</i> knockdown provoked a MET phenotype observed by crystal violet staining and verified by immunohistochemistry against E-cadherin. Wound healing assay showed no migration, and F-actin stain revealed rearrangement of actin microfilaments. In addition, <i>TGFβ1</i> and <i>TGFβ3</i> were downregulated in the absence of <i>ETV5</i>. <i>ETV5</i> silencing induces epithelial phenotype by downregulating <i>TGFβ1</i> and <i>TGFβ3</i> in INS-1 (832/13) cell line.</p>\",\"PeriodicalId\":12999,\"journal\":{\"name\":\"Hormone and Metabolic Research\",\"volume\":\" \",\"pages\":\"235-243\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2024-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Hormone and Metabolic Research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1055/a-2246-4778\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/2/9 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"ENDOCRINOLOGY & METABOLISM\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Hormone and Metabolic Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1055/a-2246-4778","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/2/9 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"ENDOCRINOLOGY & METABOLISM","Score":null,"Total":0}
ETV5 Silencing Produces Mesenchymal to Epithelial Transition in INS-1 (832/13) Cell Line.
ETV5 has been described to be involved in the epithelial to mesenchymal transition (EMT) mainly in cancer. It is known that EMT provokes cytoskeleton remodeling, improving cellular migratory, and invasive capabilities. Moreover, overexpression of ETV5 has been correlated to cancer development and this gene has been implicated in cell proliferation. However, little is known about the downregulation of ETV5 expression in a pancreatic cell line and the inverse mesenchymal to epithelial transition (MET). Therefore, we studied the implications of ETV5 silencing over the phenotype of the insulinoma INS-1 (832/13) cell line and described the MET by partial ETV5 silencing in the INS-1 (832/13) cell line. The downregulation of ETV5 expression was obtained by using ETV5 siRNA in the insulinoma rat cell line, INS-1 (832/13). Then, ETV5 knockdown provoked a MET phenotype observed by crystal violet staining and verified by immunohistochemistry against E-cadherin. Wound healing assay showed no migration, and F-actin stain revealed rearrangement of actin microfilaments. In addition, TGFβ1 and TGFβ3 were downregulated in the absence of ETV5. ETV5 silencing induces epithelial phenotype by downregulating TGFβ1 and TGFβ3 in INS-1 (832/13) cell line.
期刊介绍：
Covering the fields of endocrinology and metabolism from both, a clinical and basic science perspective, this well regarded journal publishes original articles, and short communications on cutting edge topics.
Speedy publication time is given high priority, ensuring that endocrinologists worldwide get timely, fast-breaking information as it happens.
Hormone and Metabolic Research presents reviews, original papers, and short communications, and includes a section on Innovative Methods. With a preference for experimental over observational studies, this journal disseminates new and reliable experimental data from across the field of endocrinology and metabolism to researchers, scientists and doctors world-wide.
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