Gleyce Ha de Souza, Marcia Sm Vaz, Joyce A Dos Santos Radai, Thiago L Fraga, Luana Rossato, Simone Simionatto
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引用次数: 0
摘要
目的评估香芹酚和多粘菌素 B 的协同组合对耐多粘菌素肺炎克雷伯菌的抗菌活性。方法：方法：采用棋盘试验研究协同作用、生物膜抑制评估和膜完整性试验。此外，研究还包括使用小鼠感染模型进行体内评估。结果棋盘法评估了 48 种组合，其中 23 种具有协同作用。其中，香芹酚 10 毫克/千克加多粘菌素 B 2 毫克/千克在小鼠感染模型中显示出体内抗菌活性，从而提高了存活率，并显著降低了血液中的细菌负荷。结论多粘菌素与香芹酚的协同作用是开发新型抗菌药的一种很有前途的选择。
Synergistic interaction of polymyxin B with carvacrol: antimicrobial strategy against polymyxin-resistant Klebsiella pneumoniae.
Objective: The antimicrobial activities of the synergistic combination of carvacrol and polymyxin B against polymyxin-resistant Klebsiella pneumoniae were evaluated. Methods: The methods employed checkerboard assays to investigate synergism, biofilm inhibition assessment and membrane integrity assay. In addition, the study included in vivo evaluation using a mouse infection model. Results: The checkerboard method evaluated 48 combinations, with 23 indicating synergistic action. Among these, carvacrol 10 mg/kg plus polymyxin B 2 mg/kg exhibited in vivo antimicrobial activity in a mouse model of infection, resulting in increased survival and a significant decrease in bacterial load in the blood. Conclusion: Polymyxin in synergy with carvacrol represents a promising alternative to be explored in the development of new antimicrobials.
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Future Microbiology delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this increasingly important and vast area of research.
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