微重力对在微流控芯片中生长的原发性海马神经元自发钙活动的影响

IF 4.4 1区 物理与天体物理 Q1 MULTIDISCIPLINARY SCIENCES
Pierre-Ewen Lecoq, Chloé Dupuis, Xavier Mousset, Xavier Benoit-Gonnin, Jean-Michel Peyrin, Jean-Luc Aider
{"title":"微重力对在微流控芯片中生长的原发性海马神经元自发钙活动的影响","authors":"Pierre-Ewen Lecoq, Chloé Dupuis, Xavier Mousset, Xavier Benoit-Gonnin, Jean-Michel Peyrin, Jean-Luc Aider","doi":"10.1038/s41526-024-00355-x","DOIUrl":null,"url":null,"abstract":"<p><p>The influence of variations of gravity, either hypergravity or microgravity, on the brain of astronauts is a major concern for long journeys in space, to the Moon or to Mars, or simply long-duration missions on the ISS (International Space Station). Monitoring brain activity, before and after ISS missions already demonstrated important and long term effects on the brains of astronauts. In this study, we focus on the influence of gravity variations at the cellular level on primary hippocampal neurons. A dedicated setup has been designed and built to perform live calcium imaging during parabolic flights. During a CNES (Centre National d'Etudes Spatiales) parabolic flight campaign, we were able to observe and monitor the calcium activity of 2D networks of neurons inside microfluidic devices during gravity changes over different parabolas. Our preliminary results clearly indicate a modification of the calcium activity associated to variations of gravity.</p>","PeriodicalId":54263,"journal":{"name":"npj Microgravity","volume":null,"pages":null},"PeriodicalIF":4.4000,"publicationDate":"2024-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10847089/pdf/","citationCount":"0","resultStr":"{\"title\":\"Influence of microgravity on spontaneous calcium activity of primary hippocampal neurons grown in microfluidic chips.\",\"authors\":\"Pierre-Ewen Lecoq, Chloé Dupuis, Xavier Mousset, Xavier Benoit-Gonnin, Jean-Michel Peyrin, Jean-Luc Aider\",\"doi\":\"10.1038/s41526-024-00355-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The influence of variations of gravity, either hypergravity or microgravity, on the brain of astronauts is a major concern for long journeys in space, to the Moon or to Mars, or simply long-duration missions on the ISS (International Space Station). Monitoring brain activity, before and after ISS missions already demonstrated important and long term effects on the brains of astronauts. In this study, we focus on the influence of gravity variations at the cellular level on primary hippocampal neurons. A dedicated setup has been designed and built to perform live calcium imaging during parabolic flights. During a CNES (Centre National d'Etudes Spatiales) parabolic flight campaign, we were able to observe and monitor the calcium activity of 2D networks of neurons inside microfluidic devices during gravity changes over different parabolas. Our preliminary results clearly indicate a modification of the calcium activity associated to variations of gravity.</p>\",\"PeriodicalId\":54263,\"journal\":{\"name\":\"npj Microgravity\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.4000,\"publicationDate\":\"2024-02-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10847089/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"npj Microgravity\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1038/s41526-024-00355-x\",\"RegionNum\":1,\"RegionCategory\":\"物理与天体物理\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MULTIDISCIPLINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"npj Microgravity","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1038/s41526-024-00355-x","RegionNum":1,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

重力变化(超重力或微重力)对宇航员大脑的影响是太空长途旅行(月球或火星)或国际空间站(ISS)长期任务的一个主要问题。在国际空间站任务前后对大脑活动的监测已经证明了对宇航员大脑的重要和长期影响。在这项研究中,我们重点研究重力变化在细胞层面对初级海马神经元的影响。我们设计并建造了一个专用装置,用于在抛物线飞行期间进行实时钙成像。在一次 CNES(法国国家空间研究中心)抛物线飞行活动中,我们观察并监测了不同抛物线上重力变化时微流体装置内二维神经元网络的钙活动。我们的初步结果清楚地表明,钙活动的改变与重力变化有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Influence of microgravity on spontaneous calcium activity of primary hippocampal neurons grown in microfluidic chips.

The influence of variations of gravity, either hypergravity or microgravity, on the brain of astronauts is a major concern for long journeys in space, to the Moon or to Mars, or simply long-duration missions on the ISS (International Space Station). Monitoring brain activity, before and after ISS missions already demonstrated important and long term effects on the brains of astronauts. In this study, we focus on the influence of gravity variations at the cellular level on primary hippocampal neurons. A dedicated setup has been designed and built to perform live calcium imaging during parabolic flights. During a CNES (Centre National d'Etudes Spatiales) parabolic flight campaign, we were able to observe and monitor the calcium activity of 2D networks of neurons inside microfluidic devices during gravity changes over different parabolas. Our preliminary results clearly indicate a modification of the calcium activity associated to variations of gravity.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
npj Microgravity
npj Microgravity Physics and Astronomy-Physics and Astronomy (miscellaneous)
CiteScore
7.30
自引率
7.80%
发文量
50
审稿时长
9 weeks
期刊介绍: A new open access, online-only, multidisciplinary research journal, npj Microgravity is dedicated to publishing the most important scientific advances in the life sciences, physical sciences, and engineering fields that are facilitated by spaceflight and analogue platforms.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信