侵袭性念珠菌病患者的抗念珠菌抗体可抑制生长、改变细胞壁结构并杀死体外白色念珠菌。

IF 3.6 3区 生物学 Q2 MYCOLOGY
Giulia Carrano, Inés Arrieta-Aguirre, Ander Díez, Marta Bregón-Villahoz, Iñigo Fernandez-de-Larrinoa, María-Dolores Moragues
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引用次数: 0

摘要

由念珠菌,尤其是白色念珠菌引起的侵袭性念珠菌病(IC)威胁极大,死亡率很高。由于念珠菌在人类微生物群中的普遍存在,诊断具有挑战性。为了解决这个问题,我们的研究小组开发了一种免疫荧光检测法,用于检测 IC 患者的白色念珠菌芽管抗体(CAGTA)。CAGTA 是侵入性过程的标志,与 ICU 患者较低的死亡率有关。基于这一前提,本研究旨在提供有关 CAGTA 对由真菌白色念珠菌引起的人类侵袭性感染的潜在活性的结果。因此,为了鉴定 IC 患者产生的 CAGTA 的活性,我们使用了 29 名由白念珠菌或非白念珠菌引起的 IC 患者的血清。全血清 IgG 抗体被分为抗白念珠菌、富集 CAGTA 和纯化 CAGTA,评估包括 XTT 比色法检测代谢活性,CFU 计数检测存活率,显微镜检测生长、存活率和形态分析。与抗泡孢子相比,富含 CAGTA 的 IgG 部分能显著降低白僵菌的代谢活性和存活率。电子显微镜显示,纯化的 CAGTA 改变了芽管细胞壁表面,并通过 DiBAC 荧光染色显示出杀菌特性。总之,针对侵袭性念珠菌病的抗体对白色念珠菌具有抗真菌活性,可影响新陈代谢活性、存活率和细胞壁结构,导致细胞死亡。这些研究结果表明,CAGTA 具有作为诊断标记物的潜在用途,并支持制定针对念珠菌感染的免疫方案的可能性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Anti-Candida Antibodies of Patients with Invasive Candidiasis Inhibit Growth, Alter Cell Wall Structure, and Kill Candida albicans In Vitro.

Anti-Candida Antibodies of Patients with Invasive Candidiasis Inhibit Growth, Alter Cell Wall Structure, and Kill Candida albicans In Vitro.

Invasive candidiasis (IC), caused by Candida yeasts, particularly Candida albicans, poses a significant threat with high mortality rates. Diagnosis is challenging due to Candida's common presence in human microbiota. To address this, our research group developed an immunofluorescence assay detecting Candida albicans Germ Tube Antibodies (CAGTA) in IC patients. CAGTA, indicative of invasive processes, is associated with a lower mortality rate in ICU patients. Based on this premise, this study aims to provide results regarding the lack of knowledge about the potential activity of CAGTA against invasive infections in humans caused by the fungus Candida albicans. Therefore, in order to characterize the activity of CAGTA produced by patients with IC, we used sera from 29 patients with IC caused by either C. albicans or non-albicans Candida species. Whole serum IgG antibodies were fractionated into anti-blastospores, CAGTA-enriched, and purified CAGTA and the assessments included XTT colorimetric assays for metabolic activity, CFU counts for viability, and microscopy for growth, viability, and morphological analysis. The CAGTA-enriched IgG fraction significantly reduced the metabolic activity and viability of C. albicans compared to anti-blastospores. Purified CAGTA altered germ tube cell wall surfaces, as revealed by electron microscopy, and exhibited fungicidal properties by DiBAC fluorescent staining. In conclusion, antibodies in response to invasive candidiasis have antifungal activity against Candida albicans, influencing metabolic activity, viability, and cell wall structure, leading to cell death. These findings suggest the potential utility of CAGTA as diagnostic markers and support the possibility of developing immunization protocols against Candida infections.

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来源期刊
Mycopathologia
Mycopathologia 生物-真菌学
CiteScore
6.80
自引率
3.60%
发文量
76
审稿时长
3 months
期刊介绍: Mycopathologia is an official journal of the International Union of Microbiological Societies (IUMS). Mycopathologia was founded in 1938 with the mission to ‘diffuse the understanding of fungal diseases in man and animals among mycologists’. Many of the milestones discoveries in the field of medical mycology have been communicated through the pages of this journal. Mycopathologia covers a diverse, interdisciplinary range of topics that is unique in breadth and depth. The journal publishes peer-reviewed, original articles highlighting important developments concerning medically important fungi and fungal diseases. The journal highlights important developments in fungal systematics and taxonomy, laboratory diagnosis of fungal infections, antifungal drugs, clinical presentation and treatment, and epidemiology of fungal diseases globally. Timely opinion articles, mini-reviews, and other communications are usually invited at the discretion of the editorial board. Unique case reports highlighting unprecedented progress in the diagnosis and treatment of fungal infections, are published in every issue of the journal. MycopathologiaIMAGE is another regular feature for a brief clinical report of potential interest to a mixed audience of physicians and laboratory scientists. MycopathologiaGENOME is designed for the rapid publication of new genomes of human and animal pathogenic fungi using a checklist-based, standardized format.
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