使用双相磷酸钙和去蛋白牛骨矿进行窦底增量的基因表达、显微 CT 和组织形态计量学分析：随机对照临床试验。

IF 3.7 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical Implant Dentistry and Related Research Pub Date : 2024-02-05 DOI:10.1111/cid.13303

Sirida Arunjaroensuk DDS, MSc, Katanchalee Nampuksa MSc, Naruporn Monmaturapoj PhD, Pasutha Thunyakitpisal DDS, PhD, Thantrira Porntaveetus DDS, PhD, Nikos Mattheos DDS, PhD, Atiphan Pimkhaokham DDS, PhD

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引用次数: 0

摘要

目的：本随机对照临床试验旨在比较 70/30 比值的双相磷酸钙（BCP）和去蛋白牛骨矿物质（DBBM）在上颌窦增量术中的基因表达、显微 CT 和组织形态分析：24 名需要通过侧方入路进行上颌窦底增量术的患者被随机分为 70/30 比值的 BCP 和 DBBM。在愈合至少 6 个月后，从植入种植体区域的整个增量骨高度采集 24 块骨标本，并进行显微 CT、组织形态计量学和基因表达分析。BCP 70/30 比例的 12 块骨标本被平均分配进行显微 CT 和组织学分析（试验组，n = 6）以及基因表达分析（试验组，n = 6）。同样，12 块 DBBM 骨标本也被分配进行显微 CT 和组织学分析（对照组，n = 6）以及基因表达分析（对照组，n = 6）。对新形成的骨、剩余的移植材料和四个目标基因表达的相对变化进行评估：显微计算机断层扫描结果显示，两组骨量与总体积之比（BV/TV 比）无统计学差异（BCP 41.51% vs. DBBM 40.97%），残余移植材料与总体积之比（GV/TV 比，BCP 9.97% vs. DBBM 14.41%）也是如此。同样，在骨形成（BCP 31.43% vs. DBBM 30.09%）和残余移植物面积（DBBM 40.76% vs. BCP 45.06%）方面，组织学分析也没有显示出明显的差异。在基因表达方面，两组骨移植标本的 ALP 水平均低于原生骨。相反，OSX、IL-1B 和 TRAP 的水平在两组增量骨中都高于原生骨。然而，BCP组和DBBM组之间所有基因表达的相对差异均不显著：结论：BCP、HA/β-TCP 的比例为 70/30 与 DBBM 相比，在新骨形成和残留移植物颗粒方面具有相似的组织学和显微 CT 结果。基因表达分析显示，增量骨和原生骨的基因表达模式不同，但两种生物材料之间无显著差异。

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Gene expression, micro-CT and histomorphometrical analysis of sinus floor augmentation with biphasic calcium phosphate and deproteinized bovine bone mineral: A randomized controlled clinical trial

Aims

The aim of this randomized controlled clinical trial was to compare the gene expression, micro-CT, histomorphometrical analysis between biphasic calcium phosphate (BCP) of 70/30 ratio and deproteinized bovine bone mineral (DBBM) in sinus augmentation.

Materials and Methods

Twenty-four patients in need for sinus floor augmentation through lateral approach were randomized into BCP 70/30 ratio or DBBM. After at least 6 months of healing, a total of 24 bone specimens were collected from the entire height of the augmented bone at the area of implant placement and underwent micro-CT, histomorphometric and gene expression analysis. The 12 bone specimens of BCP 70/30 ratio were equally allocated to micro-CT and histologic analysis (test group, n = 6) and gene expression analysis (test group, n = 6). Similarly, the 12 bone specimens of DBBM were also allocated to micro-CT and histologic analysis (control group, n = 6) and gene expression analysis (control group, n = 6). The newly formed bone, remaining graft materials and relative change in gene expression of four target genes were assessed.

Results

The micro-CT results showed no statistically significant difference in the ratio of bone volume to total volume (BV/TV ratio) for the two groups (BCP 41.51% vs. DBBM 40.97%) and the same was true for residual graft material to total volume (GV/TV ratio, BCP 9.97% vs. DBBM 14.41%). Similarly, no significant difference was shown in the histological analysis in terms of bone formation, (BCP 31.43% vs. DBBM was 30.09%) and residual graft area (DBBM 40.76% vs. BCP 45.06%). With regards to gene expression, the level of ALP was lower in both groups of bone grafted specimens compared with the native bone. On the contrary, the level of OSX, IL-1B and TRAP was higher in augmented bone of both groups compared with the native bone. However, the relative difference in all gene expressions between BCP and DBBM group was not significant.

Conclusions

The BCP, HA/β-TCP ratio of 70/30 presented similar histological and micro-CT outcomes in terms of new bone formation and residual graft particles with DBBM. The gene expression analysis revealed different gene expression patterns between augmented and native bone, but showed no significant difference between the two biomaterials.

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来源期刊

Clinical Implant Dentistry and Related Research 医学-牙科与口腔外科

CiteScore

6.00

自引率

13.90%

发文量

103

审稿时长

4-8 weeks

期刊介绍： The goal of Clinical Implant Dentistry and Related Research is to advance the scientific and technical aspects relating to dental implants and related scientific subjects. Dissemination of new and evolving information related to dental implants and the related science is the primary goal of our journal. The range of topics covered by the journals will include but be not limited to: New scientific developments relating to bone Implant surfaces and their relationship to the surrounding tissues Computer aided implant designs Computer aided prosthetic designs Immediate implant loading Immediate implant placement Materials relating to bone induction and conduction New surgical methods relating to implant placement New materials and methods relating to implant restorations Methods for determining implant stability A primary focus of the journal is publication of evidenced based articles evaluating to new dental implants, techniques and multicenter studies evaluating these treatments. In addition basic science research relating to wound healing and osseointegration will be an important focus for the journal.