生物凝胶中的三维牵引力显微镜:从单细胞到多细胞球。

IF 12.8 1区 工程技术 Q1 ENGINEERING, BIOMEDICAL
Brian C H Cheung, Rana J Abbed, Mingming Wu, Susan E Leggett
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引用次数: 0

摘要

细胞牵引力在引导细胞功能(如增殖、迁移和分化)方面起着至关重要的作用。目前对细胞牵引力的了解主要来自二维测量,即把细胞培养在二维基底上。然而,二维测量并不能再现生命系统的一个重要方面,即细胞会主动重塑其周围的细胞外基质(ECM),而重塑后的 ECM 又会对细胞表型和牵引力的产生产生深远的影响。生物系统的这种相互适应性体现在生物凝胶的材料特性中。在这篇综述中,我们总结了最近在测量嵌入三维生物凝胶中的细胞牵引力方面取得的进展,重点是细胞-ECM 交叉对话。我们还对可用于测量复杂生化和生物物理环境中细胞牵引力的工具和技术进行了展望。生物医学工程年度综述》第 26 卷的最终在线出版日期预计为 2024 年 5 月。修订后的预计日期请参见 http://www.annualreviews.org/page/journal/pubdates。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
3D Traction Force Microscopy in Biological Gels: From Single Cells to Multicellular Spheroids.

Cell traction force plays a critical role in directing cellular functions, such as proliferation, migration, and differentiation. Current understanding of cell traction force is largely derived from 2D measurements where cells are plated on 2D substrates. However, 2D measurements do not recapitulate a vital aspect of living systems; that is, cells actively remodel their surrounding extracellular matrix (ECM), and the remodeled ECM, in return, can have a profound impact on cell phenotype and traction force generation. This reciprocal adaptivity of living systems is encoded in the material properties of biological gels. In this review, we summarize recent progress in measuring cell traction force for cells embedded within 3D biological gels, with an emphasis on cell-ECM cross talk. We also provide perspectives on tools and techniques that could be adapted to measure cell traction force in complex biochemical and biophysical environments.

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来源期刊
Annual Review of Biomedical Engineering
Annual Review of Biomedical Engineering 工程技术-工程:生物医学
CiteScore
18.80
自引率
0.00%
发文量
14
期刊介绍: Since 1999, the Annual Review of Biomedical Engineering has been capturing major advancements in the expansive realm of biomedical engineering. Encompassing biomechanics, biomaterials, computational genomics and proteomics, tissue engineering, biomonitoring, healthcare engineering, drug delivery, bioelectrical engineering, biochemical engineering, and biomedical imaging, the journal remains a vital resource. The current volume has transitioned from gated to open access through Annual Reviews' Subscribe to Open program, with all articles published under a CC BY license.
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