合成肽 ∆M3 对广谱β-内酰胺酶大肠埃希菌分离物的膜活性研究。

IF 2.3 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Journal of Membrane Biology Pub Date : 2024-04-01 Epub Date: 2024-02-05 DOI:10.1007/s00232-024-00306-3
Estefanía Fandiño-Devia, Gloria A Santa-González, Maria C Klaiss-Luna, Marcela Manrique-Moreno
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引用次数: 0

摘要

大肠埃希菌是引起医院或社区获得性菌血症的最常见微生物,全球范围内发现的产扩展谱β-内酰胺酶的大肠埃希菌分离株越来越多。因此,有必要对抗菌肽等潜在的新分子进行评估。抗菌肽具有公认的生物潜力,因此在抗感染方面很有前途。这项研究的目的是评估合成肽 ΔM3 对几种产生广谱 β 内酰胺酶的大肠杆菌分离物的潜力。用分光光度法测定了该肽的抗菌和细胞毒性活性。此外,还通过荧光显微镜和红外光谱监测了多肽与细菌膜相互作用的能力。结果表明,在浓度与美罗培南相似的情况下,合成肽对大肠杆菌分离株具有活性。另一方面,在 HaCaT 角质细胞中,即使浓度为最小抑制浓度的 10 倍,也没有观察到细胞毒性作用。显微镜检查结果表明,多肽对细菌有渗透作用。红外线结果表明,ΔM3 对微生物膜的脂质具有亲和力。结果表明,ΔM3 与大肠杆菌带负电荷的脂质相互作用,对膜产生干扰作用。最后,多肽的二级结构实验表明,它在溶液中的结构是随机的,在与膜相互作用的过程中并没有发生变化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Study of the Membrane Activity of the Synthetic Peptide ∆M3 Against Extended-Spectrum β-lactamase Escherichia coli Isolates.

Study of the Membrane Activity of the Synthetic Peptide ∆M3 Against Extended-Spectrum β-lactamase Escherichia coli Isolates.

Escherichia coli is the most common microorganism causing nosocomial or community-acquired bacteremia, and extended-spectrum β-lactamase-producing Escherichia coli isolates are identified worldwide with increasing frequency. For this reason, it is necessary to evaluate potential new molecules like antimicrobial peptides. They are recognized for their biological potential which makes them promising candidates in the fight against infections. The goal of this research was to evaluate the potential of the synthetic peptide ΔM3 on several extended-spectrum β-lactamase producing E. coli isolates. The antimicrobial and cytotoxic activity of the peptide was spectrophotometrically determined. Additionally, the capacity of the peptide to interact with the bacterial membrane was monitored by fluorescence microscopy and infrared spectroscopy. The results demonstrated that the synthetic peptide is active against Escherichia coli isolates at concentrations similar to Meropenem. On the other hand, no cytotoxic effect was observed in HaCaT keratinocyte cells even at 10 times the minimal inhibitory concentration. Microscopy results showed a permeabilizing effect of the peptide on the bacteria. The infrared results showed that ΔM3 showed affinity for the lipids of the microorganism's membrane. The results suggest that the ∆M3 interacts with the negatively charged lipids from the E. coli by a disturbing effect on membrane. Finally, the secondary structure experiments of the peptide showed a random structure in solution that did not change during the interaction with the membranes.

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来源期刊
Journal of Membrane Biology
Journal of Membrane Biology 生物-生化与分子生物学
CiteScore
4.80
自引率
4.20%
发文量
63
审稿时长
6-12 weeks
期刊介绍: The Journal of Membrane Biology is dedicated to publishing high-quality science related to membrane biology, biochemistry and biophysics. In particular, we welcome work that uses modern experimental or computational methods including but not limited to those with microscopy, diffraction, NMR, computer simulations, or biochemistry aimed at membrane associated or membrane embedded proteins or model membrane systems. These methods might be applied to study topics like membrane protein structure and function, membrane mediated or controlled signaling mechanisms, cell-cell communication via gap junctions, the behavior of proteins and lipids based on monolayer or bilayer systems, or genetic and regulatory mechanisms controlling membrane function. Research articles, short communications and reviews are all welcome. We also encourage authors to consider publishing ''negative'' results where experiments or simulations were well performed, but resulted in unusual or unexpected outcomes without obvious explanations. While we welcome connections to clinical studies, submissions that are primarily clinical in nature or that fail to make connections to the basic science issues of membrane structure, chemistry and function, are not appropriate for the journal. In a similar way, studies that are primarily descriptive and narratives of assays in a clinical or population study are best published in other journals. If you are not certain, it is entirely appropriate to write to us to inquire if your study is a good fit for the journal.
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