研究正常男性和少精子症男性精浆外泌体在植入过程中的影响

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Hadis Gholipour, Fatemeh Sadat Amjadi, Zahra Zandieh, Mehdi Mehdizadeh, Marziyeh Ajdary, Ali Akbar Delbandi, Azadeh Akbari Sene, Reza Aflatoonian, Mehrdad Bakhtiyari
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引用次数: 0

摘要

背景:精浆外泌体目前被认为在女性生殖系统不孕症的调控中发挥着复杂的作用。本研究的目的是评估从少精子症男性精子中提取的外泌体对子宫内膜植入相关基因的影响:为了分离外泌体,我们对10名精子参数正常的育龄男性和10名少精子症男性的精子样本采用了超速离心法。然后利用透射电子显微镜和动态光散射法对外泌体的大小分布和超微结构进行了鉴定。我们用 Western 印迹分析检测了外泌体标记物,并用 DiI 染料孵育外泌体,用荧光显微镜观察外泌体,从而确认了外泌体在细胞质中的定位。用 100 µg/ml 精液外泌体体外处理子宫内膜上皮细胞 6 小时后,使用 qRT-PCR 检测子宫内膜接受性基因。我们使用 Image J 和 Prism 软件进行数据分析和量化。P<0.05为差异有统计学意义:结果:与正常组相比,经少精子外泌体处理的子宫内膜上皮细胞在处理6小时后,MUC1、LIF、G-CSF、CX3CL1和VEGF的mRNA水平明显下调。虽然与正常组相比,少精症组IL8和TGF-β基因的平均mRNA水平发生了变化,但这些差异未达到统计学意义(P > 0.05):结论:与正常外泌体相比,少精症外泌体对子宫内膜接受性有明显影响,导致植入相关基因表达减少。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Investigation of the Effect of Seminal Plasma Exosomes from the Normal and Oligoasthenoteratospermic Males in the Implantation Process.

Background: Seminal plasma exosomes are now recognized to play a complex role in the regulation of the female reproductive system infertility. The objective of this study was to assess the effect of exosomes derived from the sperm of men with oligoasthenoteratozoospermia on endometrial implantation-related genes.

Methods: To isolate the exosomes, we employed an ultracentrifugation method on samples derived from 10 fertile men with normal sperm parameters and 10 men with oligoasthenoteratozoospermia. The size distribution and ultrastructure of the exosomes were then characterized using transmission electron microscopy and dynamic light scattering. We detected an exosome marker using western blot analysis and confirmed the cytoplasmic localization of the exosomes by incubating them with DiI dye and visualizing them using fluorescence microscopy. After 6 hours of in vitro treatment of endometrial epithelial cells with 100 µg/ml seminal exosome, the endometrial receptivity genes were examined using qRT-PCR. To perform data analysis and quantification, we utilized Image J and Prism software. P< 0.05 were considered statistically significant.

Results: After 6 hours of treatment, the mRNA levels of MUC1, LIF, G-CSF, CX3CL1, and VEGF were significantly downregulated in the endometrial epithelial cells treated with oligoasthenoteratozoospermia exosomes compared to the normal group. Although changes were observed in the mean mRNA levels of IL8 and TGF-β genes in the oligoasthenoteratozoospermia group compared to the normal group, these differences did not reach statistical significance (p > 0.05).

Conclusions: Oligoasthenoteratozoospermia exosomes have a distinct effect on endometrial receptivity compared to normal exosomes, leading to reduced expression of implantation-related genes.

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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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