具有藻酸盐涂层的金银核壳纳米结构能诱导人淋巴细胞肿瘤(Jurkat)细胞株凋亡吗?

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Jamileh Sadat Mirsanei, Mahsa Nazari, Ronak Shabani, Azam Govahi, Sahar Eghbali, Marziyeh Ajdary, Rana Mehdizadeh, Atieh Sadat Mousavi, Mehdi Mehdizadeh
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引用次数: 0

摘要

背景:众所周知,T 细胞急性淋巴细胞白血病(T-ALL)是一种侵袭性恶性疾病,由 T 前体细胞的肿瘤性改变引起。虽然严格的化疗方案在儿童中的治愈率高达 80%,但在成人治疗中的成功率却较低。银纳米粒子(Ag-NPs)在体外对人类乳腺癌细胞、人类胶质母细胞瘤 U251 细胞和慢性粒细胞白血病细胞有毒性作用。本研究旨在探讨 Ag 纳米结构(Ag-NSs)对 Jurkat 细胞活力和凋亡的影响:方法:获得 Jurkat 细胞系。方法:以 Jurkat 细胞系为研究对象,在合成 Ag-NSs 并对其进行表征后,以不同剂量将其与 Jurkat 细胞培养 24、48 和 72 小时,以确定最佳时间和剂量。实验分为两组:对照组和处理组,对照组的 Jurkat 细胞不含纳米结构,与处理组的细胞保持在相同的培养基中,不更换培养基;处理组的细胞根据 MTT 结果,用 75 µg/ml 剂量的 Ag 纳米结构溶液处理 48 小时。48 小时后,对两组细胞的凋亡基因(BAX、BCL-2 和 CASPASE-3)进行 q RT-PCR:结果:根据我们的研究结果,棒状银纳米结构的尺寸约为 50 纳米,能增加细胞凋亡标志物,包括 BAX 和 CASPASE-3,并诱导细胞死亡:Ag-NS具有抗癌特性,能诱导细胞凋亡,因此可能成为治疗T细胞急性淋巴细胞白血病的潜在候选药物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Does Gold-Silver Core-Shell Nanostructure with Alginate Coating Induce Apoptosis in Human Lymphoblastic Tumoral (Jurkat) Cell Line?

Background: T-cell acute lymphoblastic leukemia (T-ALL) is known as an aggressive malignant disease resulting from the neoplastic alteration of T precursor cells. Although treatment with stringent chemotherapy regimens has achieved an 80% cure rate in children, it has been associated with lower success rates in adult treatment. Silver nanoparticles (Ag-NPs) have a toxic effect on human breast cancer cells, human glioblastoma U251 cells, and chronic myeloid leukemia cells in vitro. This study aimed to investigate the effect of Ag nanostructures (Ag-NSs) on Jurkat cells' viability and apoptosis.

Methods: The Jurkat cell line was acquired. Following the synthesis Ag-NSs and their characterization, they were incubated with Jurkat cells at different doses for 24, 48, and 72 hours to determine the optimal time and dose. Two groups were examined: a control group with Jurkat cells without nanostructure maintained in the same medium as the cells in the treatment group without changing the medium, and a treatment group with cells treated with the Ag nanostructure solution at a dose of 75 µg/ml for 48 hours according to the MTT results. After 48 hours, the cells from the two groups were used for the q RT-PCR of the apoptotic genes (BAX, BCL-2, and CASPASE-3).

Results: According to our results, the rod-shaped silver nanostructures had a size of about 50 nm, increased apoptotic markers, including BAX and CASPASE-3, and induced cell death.

Conclusions: Ag-NSs have anticancer properties and can induce apoptosis of cells; therefore, they may be a potential candidate for the treatment of T-cell acute lymphoblastic leukemia.

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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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