循环 miR-21 过表达与系统性红斑狼疮(SLE)中的 PDCD4 和 IL-10 相关:一种有前景的诊断和预后生物标志物

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Nibras Kamil Alhassbalawi, Mojtaba Zare Ebrahimabad, Fakhri Sadat Seyedhosseini, Yasser Bagheri, Nafiseh Abdollahi, Alireza Nazari, Saeed Mohammadi, Yaghoub Yazdani
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引用次数: 0

摘要

背景:系统性红斑狼疮(SLE)是一种影响多个器官的慢性自身免疫性疾病,对发病率和死亡率有重大影响。系统性红斑狼疮的发病受遗传易感性和免疫反应失调的影响。我们的目的是研究系统性红斑狼疮患者血浆中 miR-21、IL-10 和 PDCD4 的表达,并分析它们之间的相关性以及潜在的诊断和预后价值:研究对象包括100名健康受试者、50名新诊断(ND)和50名治疗不足(UT)的系统性红斑狼疮患者。结果:系统性红斑狼疮患者的 miR-21 和 IL-10 表达水平显著高于健康受试者,其中 ND 患者的表达水平最高。系统性红斑狼疮患者的 PDCD4 表达水平也明显高于健康受试者,其中UT 患者的表达水平最高。ROC 曲线分析和 Cox-Mantel Log-rank 检验表明,miR-21、PDCD4 和 IL-10 是诊断系统性红斑狼疮和预后的适当生物标志物。研究还发现,系统性红斑狼疮患者的 miR-21 和 PDCD4 与 IL-10 水平之间存在明显的正相关性:这些研究表明,系统性红斑狼疮患者体内的 miR-21、PDCD4 和 IL-10 失调可能会导致疾病的发展,并提供了新的诊断和预后标志物。此外,在系统性红斑狼疮患者中观察到的 miR-21、PDCD4 和 IL-10 水平之间的相关性表明这些分子之间可能存在相互作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Circulating miR-21 Overexpression Correlates with PDCD4 and IL-10 in Systemic Lupus Erythematosus (SLE): A Promising Diagnostic and Prognostic Biomarker.

Background: Systemic Lupus Erythematosus (SLE) is a chronic autoimmune condition that affects multiple organs significantly impacts morbidity and mortality. The development of SLE is influenced by genetic predisposition and dysregulated immune response. Our objective was to investigate miR-21, IL-10, and PDCD4 expression in SLE patient plasma and analyze their correlations and potential diagnostic and prognostic values.

Methods: The study included 100 healthy subjects, 50 newly diagnosed (ND), and 50 under-treatment (UT) SLE patients. The patients were observed for 24 weeks to track relapses. miR-21 and PDCD4 gene expression levels were measured using real-time RT-PCR, and IL-10 production was measured using ELISA.

Results: miR-21 and IL-10 expression levels were significantly greater in SLE patients than in healthy subjects, with the highest levels observed in ND patients. PDCD4 expression was also significantly greater in SLE patients than in subjects, with the highest levels observed in UT patients. ROC curve analyses and Cox-Mantel Log-rank tests indicated miR-21, PDCD4, and IL-10 as proper diagnostic and prognostic biomarkers for SLE. The study also revealed a significant positive correlation between miR-21 and PDCD4 and IL-10 levels in SLE patients.

Conclusions: The studies suggest that dysregulation of miR-21, PDCD4, and IL-10 in patients with SLE may contribute to disease development and provides new diagnostic and prognostic markers. Additionally, the observed correlation between miR-21, PDCD4, and IL-10 levels in SLE patients signifies a potential interplay between these molecules.

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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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