{"title":"开发和优化 Teratea Clitoria 合成银纳米粒子，并将其应用于伤口愈合纳米凝胶系统。","authors":"Richa Jain, Ruchi Singh, Reena Badhwar, Tinku Gupta, Harvinder Popli","doi":"10.1080/03639045.2024.2308043","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>The present research deals with sequential optimization strategy based on Central Composite Design to optimize the process variables for efficient production of <i>Clitoria teratea</i> (CLT) synthesized silver nanoparticles (AgNPs) using biological synthesis.</p><p><strong>Methods: </strong>Two substantial factors influencing the dependent variables viz UV-visible absorbance, particle size, zeta potential and polydispersity index (PDI) were identified as NaOH concentration, RH concentration, temperature as independent variables. <i>In-vitro</i> and <i>ex-vivo</i> studies of prepared CLT-AgNPs gel and marketed gel were carried out using dialysis membrane and egg membrane, respectively. In addition, antimicrobial study was also performed on the bacterial strains.</p><p><strong>Results: </strong>The particles size (114 nm), PDI (0.45), and zeta potential (-29.5 mV) of optimized formulation were found, respectively. <i>In-vitro</i> profile of AgNPs from prepared CLT-AgNPs gel was noted (95.6%) in 8 h. It was found that the prepared CLT-AgNPs gel stimulates fibroblast and agranulocytosis development resulting better and timely wound healing.</p><p><strong>Conclusions: </strong>The prepared CLT-AgNPs gel can be as a potential substitute in the management and treatment of acute and chronic wounds.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Development and optimization of <i>Clitoria teratea</i> synthesized silver nanoparticles and its application to nanogel systems for wound healing.\",\"authors\":\"Richa Jain, Ruchi Singh, Reena Badhwar, Tinku Gupta, Harvinder Popli\",\"doi\":\"10.1080/03639045.2024.2308043\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>The present research deals with sequential optimization strategy based on Central Composite Design to optimize the process variables for efficient production of <i>Clitoria teratea</i> (CLT) synthesized silver nanoparticles (AgNPs) using biological synthesis.</p><p><strong>Methods: </strong>Two substantial factors influencing the dependent variables viz UV-visible absorbance, particle size, zeta potential and polydispersity index (PDI) were identified as NaOH concentration, RH concentration, temperature as independent variables. <i>In-vitro</i> and <i>ex-vivo</i> studies of prepared CLT-AgNPs gel and marketed gel were carried out using dialysis membrane and egg membrane, respectively. In addition, antimicrobial study was also performed on the bacterial strains.</p><p><strong>Results: </strong>The particles size (114 nm), PDI (0.45), and zeta potential (-29.5 mV) of optimized formulation were found, respectively. <i>In-vitro</i> profile of AgNPs from prepared CLT-AgNPs gel was noted (95.6%) in 8 h. It was found that the prepared CLT-AgNPs gel stimulates fibroblast and agranulocytosis development resulting better and timely wound healing.</p><p><strong>Conclusions: </strong>The prepared CLT-AgNPs gel can be as a potential substitute in the management and treatment of acute and chronic wounds.</p>\",\"PeriodicalId\":2,\"journal\":{\"name\":\"ACS Applied Bio Materials\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.6000,\"publicationDate\":\"2024-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Applied Bio Materials\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1080/03639045.2024.2308043\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/2/19 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"MATERIALS SCIENCE, BIOMATERIALS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Bio Materials","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/03639045.2024.2308043","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/2/19 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
Development and optimization of Clitoria teratea synthesized silver nanoparticles and its application to nanogel systems for wound healing.
Objective: The present research deals with sequential optimization strategy based on Central Composite Design to optimize the process variables for efficient production of Clitoria teratea (CLT) synthesized silver nanoparticles (AgNPs) using biological synthesis.
Methods: Two substantial factors influencing the dependent variables viz UV-visible absorbance, particle size, zeta potential and polydispersity index (PDI) were identified as NaOH concentration, RH concentration, temperature as independent variables. In-vitro and ex-vivo studies of prepared CLT-AgNPs gel and marketed gel were carried out using dialysis membrane and egg membrane, respectively. In addition, antimicrobial study was also performed on the bacterial strains.
Results: The particles size (114 nm), PDI (0.45), and zeta potential (-29.5 mV) of optimized formulation were found, respectively. In-vitro profile of AgNPs from prepared CLT-AgNPs gel was noted (95.6%) in 8 h. It was found that the prepared CLT-AgNPs gel stimulates fibroblast and agranulocytosis development resulting better and timely wound healing.
Conclusions: The prepared CLT-AgNPs gel can be as a potential substitute in the management and treatment of acute and chronic wounds.
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