番红花提取物可抑制高脂饮食小鼠皮下脂肪组织肥大,并改善全身胰岛素敏感性。

IF 5 3区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
BioFactors Pub Date : 2024-02-06 DOI:10.1002/biof.2043
Biljana Bursać, Luisa Bellachioma, Ljupka Gligorovska, Mirna Jovanović, Ana Teofilović, Miloš Vratarić, Danijela Vojnović Milutinović, Alfonso Albacete, Purificación A. Martínez-Melgarejo, Camilla Morresi, Elisabetta Damiani, Tiziana Bacchetti, Ana Djordjevic
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引用次数: 0

摘要

肥胖症是全球亟待解决的问题,标准治疗策略的效果有限。使用天然产品似乎是缓解肥胖症及其相关并发症的一种很有前景的方法。藏红花(Crocus sativus,Cr)植物的花被(通常在藏红花生产中被浪费)是一种尚未开发的生物活性化合物来源。我们的目的是通过研究番红花提取物对脂肪细胞分化、内脏脂肪组织(VAT)和皮下脂肪组织(SAT)肥大以及饮食诱导肥胖动物模型脂质代谢的影响,阐明番红花提取物在肥胖症中的作用机制。为此,小鼠 3T3-F442A 前脂肪细胞经山奈酚提取物处理,并测定脂肪细胞分化基因的表达。对以 60% 脂肪饮食喂养 14 周、并在饮食的最后 5 周口服 Cr tepals 提取物的小鼠的热量摄入、体重、甘油三酯、全身胰岛素敏感性、组织学、胰岛素信号转导以及 VAT 和 SAT 的脂质代谢进行了分析。我们首次在体外证明了牛樟脑提取物能抑制脂肪细胞分化。动物模型证实,口服 Cr tepals 提取物能减轻体重、改善全身胰岛素敏感性、降低甘油三酯和改善脂质过氧化反应。只有在 SAT 中才能观察到 Cr tepals 提取物对脂肪细胞肥大和炎症的抑制作用,加上 SAT 胰岛素信号的保留,很可能有助于改善全身胰岛素敏感性。我们的研究结果表明,SAT 是治疗肥胖症及其并发症的可能靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Crocus sativus tepals extract suppresses subcutaneous adipose tissue hypertrophy and improves systemic insulin sensitivity in mice on high-fat diet

Crocus sativus tepals extract suppresses subcutaneous adipose tissue hypertrophy and improves systemic insulin sensitivity in mice on high-fat diet

Crocus sativus tepals extract suppresses subcutaneous adipose tissue hypertrophy and improves systemic insulin sensitivity in mice on high-fat diet

Obesity is a pressing problem worldwide for which standard therapeutic strategies have limited effectiveness. The use of natural products seems to be a promising approach to alleviate obesity and its associated complications. The tepals of Crocus sativus (Cr) plant, usually wasted in saffron production, are an unexplored source of bioactive compounds. Our aim was to elucidate the mechanisms of Cr tepals extract in obesity by investigating its effects on adipocyte differentiation, visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) hypertrophy, and lipid metabolism in an animal model of diet-induced obesity. To this end, mouse 3T3-F442A preadipocytes were treated with Cr tepals extract and the expression of adipocyte differentiation genes was determined. Caloric intake, body mass, triglycerides, systemic insulin sensitivity, histology, insulin signaling, and lipid metabolism in VAT and SAT were analyzed in mice fed a 60% fat diet for 14 weeks and treated orally with Cr tepals extract during the last 5 weeks of the diet. We demonstrated for the first time that Cr tepals extract inhibits adipocyte differentiation in vitro. The animal model confirmed that oral treatment with Cr tepals extract results in weight loss, improved systemic insulin sensitivity, lower triglycerides, and improved lipid peroxidation. The suppressive effect of Cr tepals extract on adipocyte hypertrophy and inflammation was observed only in SAT, which, together with preserved SAT insulin signaling, most likely contributed to improved systemic insulin sensitivity. Our results suggest the functionality of SAT as a possible target for the treatment of obesity and its complications.

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来源期刊
BioFactors
BioFactors 生物-内分泌学与代谢
CiteScore
11.50
自引率
3.30%
发文量
96
审稿时长
6-12 weeks
期刊介绍: BioFactors, a journal of the International Union of Biochemistry and Molecular Biology, is devoted to the rapid publication of highly significant original research articles and reviews in experimental biology in health and disease. The word “biofactors” refers to the many compounds that regulate biological functions. Biological factors comprise many molecules produced or modified by living organisms, and present in many essential systems like the blood, the nervous or immunological systems. A non-exhaustive list of biological factors includes neurotransmitters, cytokines, chemokines, hormones, coagulation factors, transcription factors, signaling molecules, receptor ligands and many more. In the group of biofactors we can accommodate several classical molecules not synthetized in the body such as vitamins, micronutrients or essential trace elements. In keeping with this unified view of biochemistry, BioFactors publishes research dealing with the identification of new substances and the elucidation of their functions at the biophysical, biochemical, cellular and human level as well as studies revealing novel functions of already known biofactors. The journal encourages the submission of studies that use biochemistry, biophysics, cell and molecular biology and/or cell signaling approaches.
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