N. N. Novikova, M. V. Kovalchuk, A. V. Rogachev, Yu. N. Malakhova, J. O. Kotova, S. E. Gelperina, S. N. Yakunin
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引用次数: 0
摘要
摘要 使用掠入射 X 射线衍射 (XRD) 和 X 射线反射 (XRR) 研究了抗癌抗生素多柔比星与脂质细胞膜模型相互作用的分子机制。模型系统是与动物细胞膜主要成分相关的四种磷脂单层。研究获得了多柔比星对磷脂单层晶格破坏过程的新信息。研究证实,多柔比星对阴离子磷脂单层的作用是由静电作用决定的:带正电荷的多柔比星分子被结合到带负电荷的磷脂功能基团之间。在中性磷脂中,关键作用属于疏水相互作用:多柔比星分子在无序区域与磷脂碳氢化合物尾部配位。
Structural Reorganization of Cell Membrane Models Caused by the Anticancer Antibiotic Doxorubicin
Abstract
The molecular mechanisms of the interaction of anticancer antibiotic doxorubicin with lipid cell membrane models have been investigated using grazing incidence X-ray diffraction (XRD) and X-ray reflectivity (XRR). The model systems were monolayers of four types of phospholipids, related to the main components of animal cell membranes. New information on the processes of damage of phospholipid monolayer lattice caused by doxorubicin is obtained. It is established that the action of doxorubicin on anionic phospholipid monolayers is determined by the electrostatic interaction: positively charged doxorubicin molecules are incorporated between negatively charged phospholipid functional groups. In the case of neutral phospholipids the key role belongs to the hydrophobic interaction: doxorubicin molecules are coordinated with phospholipid hydrocarbon tails in disordered regions.
期刊介绍:
Crystallography Reports is a journal that publishes original articles short communications, and reviews on various aspects of crystallography: diffraction and scattering of X-rays, electrons, and neutrons, determination of crystal structure of inorganic and organic substances, including proteins and other biological substances; UV-VIS and IR spectroscopy; growth, imperfect structure and physical properties of crystals; thin films, liquid crystals, nanomaterials, partially disordered systems, and the methods of studies.