T. N. Safonova, A. N. Antipov, V. P. Veiko, N. N. Mordkovich, N. A. Okorokova, P. V. Dorovatovskii, K. M. Polyakov
{"title":"卤代嗜碱细菌 Halomonas chromatireducens 的嘌呤核苷磷酸酶的初步 X 射线衍射分析","authors":"T. N. Safonova, A. N. Antipov, V. P. Veiko, N. N. Mordkovich, N. A. Okorokova, P. V. Dorovatovskii, K. M. Polyakov","doi":"10.1134/S106377452360093X","DOIUrl":null,"url":null,"abstract":"<p>Crystals of the enzyme purine nucleoside phosphorylase from the extremophilic bacterium <i>Halomonas Chromatireducens</i> AGD 8-3, suitable for X-ray diffraction, were grown by the vapor-diffusion method. The X-ray diffraction data were collected from these crystals at the Belok beamline of the Kurchatov synchrotron radiation source (National Research Centre “Kurchatov Institute”) at 100 K to 1.80 Å resolution. The X-ray diffraction data were processed in the space groups <i>P</i>1, <i>P</i>2, <i>P</i>2<sub>1</sub>, and <i>P</i>622. The structure was solved by the molecular replacement method taking into account the twinning in the space groups <i>P</i>2<sub>1</sub> and <i>P</i>1 with one and two hexamers of the enzyme per asymmetric unit, respectively.</p>","PeriodicalId":527,"journal":{"name":"Crystallography Reports","volume":null,"pages":null},"PeriodicalIF":0.6000,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Preliminary X-ray Diffraction Analysis of Purine Nucleoside Phosphorylase from the Haloalkaliphilic Bacterium Halomonas chromatireducens\",\"authors\":\"T. N. Safonova, A. N. Antipov, V. P. Veiko, N. N. Mordkovich, N. A. Okorokova, P. V. Dorovatovskii, K. M. Polyakov\",\"doi\":\"10.1134/S106377452360093X\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Crystals of the enzyme purine nucleoside phosphorylase from the extremophilic bacterium <i>Halomonas Chromatireducens</i> AGD 8-3, suitable for X-ray diffraction, were grown by the vapor-diffusion method. The X-ray diffraction data were collected from these crystals at the Belok beamline of the Kurchatov synchrotron radiation source (National Research Centre “Kurchatov Institute”) at 100 K to 1.80 Å resolution. The X-ray diffraction data were processed in the space groups <i>P</i>1, <i>P</i>2, <i>P</i>2<sub>1</sub>, and <i>P</i>622. The structure was solved by the molecular replacement method taking into account the twinning in the space groups <i>P</i>2<sub>1</sub> and <i>P</i>1 with one and two hexamers of the enzyme per asymmetric unit, respectively.</p>\",\"PeriodicalId\":527,\"journal\":{\"name\":\"Crystallography Reports\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.6000,\"publicationDate\":\"2024-02-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Crystallography Reports\",\"FirstCategoryId\":\"88\",\"ListUrlMain\":\"https://link.springer.com/article/10.1134/S106377452360093X\",\"RegionNum\":4,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"CRYSTALLOGRAPHY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Crystallography Reports","FirstCategoryId":"88","ListUrlMain":"https://link.springer.com/article/10.1134/S106377452360093X","RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CRYSTALLOGRAPHY","Score":null,"Total":0}
引用次数: 0
摘要
摘要通过蒸发扩散法从嗜极细菌 Halomonas Chromatireducens AGD 8-3 中生长出适合 X 射线衍射的嘌呤核苷磷酸化酶晶体。这些晶体的 X 射线衍射数据是在库尔恰托夫同步辐射源（国家研究中心 "库尔恰托夫研究所"）的 Belok 光束线以 100 K 的分辨率 1.80 Å 采集的。X 射线衍射数据在空间群 P1、P2、P21 和 P622 中进行了处理。考虑到 P21 和 P1 空间群中的孪生结构，每个不对称单元分别有一个和两个酶的六聚体，因此采用分子置换法对该结构进行了求解。
Preliminary X-ray Diffraction Analysis of Purine Nucleoside Phosphorylase from the Haloalkaliphilic Bacterium Halomonas chromatireducens
Crystals of the enzyme purine nucleoside phosphorylase from the extremophilic bacterium Halomonas Chromatireducens AGD 8-3, suitable for X-ray diffraction, were grown by the vapor-diffusion method. The X-ray diffraction data were collected from these crystals at the Belok beamline of the Kurchatov synchrotron radiation source (National Research Centre “Kurchatov Institute”) at 100 K to 1.80 Å resolution. The X-ray diffraction data were processed in the space groups P1, P2, P21, and P622. The structure was solved by the molecular replacement method taking into account the twinning in the space groups P21 and P1 with one and two hexamers of the enzyme per asymmetric unit, respectively.
期刊介绍：
Crystallography Reports is a journal that publishes original articles short communications, and reviews on various aspects of crystallography: diffraction and scattering of X-rays, electrons, and neutrons, determination of crystal structure of inorganic and organic substances, including proteins and other biological substances; UV-VIS and IR spectroscopy; growth, imperfect structure and physical properties of crystals; thin films, liquid crystals, nanomaterials, partially disordered systems, and the methods of studies.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
