Fengying Yuan, Qiao Zhao, Yanyan Zeng, Xu Fang Liao, Jiali Li, Bo Liu, Jun Feng Kou, Xiaolin Zhong, Xiang Hua Wu, Jun Feng Zhang and Wen Xiu Ren
{"title":"用于活细胞、秀丽隐杆线虫和斑马鱼硫化氢检测的 COX2 靶向癌症特异性荧光探针","authors":"Fengying Yuan, Qiao Zhao, Yanyan Zeng, Xu Fang Liao, Jiali Li, Bo Liu, Jun Feng Kou, Xiaolin Zhong, Xiang Hua Wu, Jun Feng Zhang and Wen Xiu Ren","doi":"10.1039/D3AN01847D","DOIUrl":null,"url":null,"abstract":"<p >A novel cyclooxygenase-2 (COX-2) targeted H<small><sub>2</sub></small>S-activated cancer-specific fluorescent probe, namely, <strong>COX2-H<small><sub>2</sub></small>S</strong>, was designed and synthesized, with naphthalimide as the fluorophore and indomethacin as the targeting group. This H<small><sub>2</sub></small>S-sensing probe was developed to differentiate tumor cells from normal cells and was tested in living cells, <em>Caenorhabditis elegans</em> (<em>C. elegans</em>), and zebrafish. The probe could successfully be used for imaging endogenous and exogenous H<small><sub>2</sub></small>S in living cells, demonstrating high sensitivity and specificity and strong anti-interference. <strong>COX2-H<small><sub>2</sub></small>S</strong> had the ability to not only discern cancer cells from normal cells but also specifically recognize 9L/lacZ cells from other glioblastoma cells (U87-MG and LN229). It could also be successfully applied for the fluorescent live imaging of H<small><sub>2</sub></small>S in both <em>C. elegans</em> and zebrafish.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 5","pages":" 1489-1495"},"PeriodicalIF":3.3000,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A COX2-targeting cancer-specific fluorescent probe for hydrogen sulfide detection in living cells, Caenorhabditis elegans, and zebrafish†\",\"authors\":\"Fengying Yuan, Qiao Zhao, Yanyan Zeng, Xu Fang Liao, Jiali Li, Bo Liu, Jun Feng Kou, Xiaolin Zhong, Xiang Hua Wu, Jun Feng Zhang and Wen Xiu Ren\",\"doi\":\"10.1039/D3AN01847D\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >A novel cyclooxygenase-2 (COX-2) targeted H<small><sub>2</sub></small>S-activated cancer-specific fluorescent probe, namely, <strong>COX2-H<small><sub>2</sub></small>S</strong>, was designed and synthesized, with naphthalimide as the fluorophore and indomethacin as the targeting group. This H<small><sub>2</sub></small>S-sensing probe was developed to differentiate tumor cells from normal cells and was tested in living cells, <em>Caenorhabditis elegans</em> (<em>C. elegans</em>), and zebrafish. The probe could successfully be used for imaging endogenous and exogenous H<small><sub>2</sub></small>S in living cells, demonstrating high sensitivity and specificity and strong anti-interference. <strong>COX2-H<small><sub>2</sub></small>S</strong> had the ability to not only discern cancer cells from normal cells but also specifically recognize 9L/lacZ cells from other glioblastoma cells (U87-MG and LN229). It could also be successfully applied for the fluorescent live imaging of H<small><sub>2</sub></small>S in both <em>C. elegans</em> and zebrafish.</p>\",\"PeriodicalId\":63,\"journal\":{\"name\":\"Analyst\",\"volume\":\" 5\",\"pages\":\" 1489-1495\"},\"PeriodicalIF\":3.3000,\"publicationDate\":\"2024-02-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analyst\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://pubs.rsc.org/en/content/articlelanding/2024/an/d3an01847d\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analyst","FirstCategoryId":"92","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2024/an/d3an01847d","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
A COX2-targeting cancer-specific fluorescent probe for hydrogen sulfide detection in living cells, Caenorhabditis elegans, and zebrafish†
A novel cyclooxygenase-2 (COX-2) targeted H2S-activated cancer-specific fluorescent probe, namely, COX2-H2S, was designed and synthesized, with naphthalimide as the fluorophore and indomethacin as the targeting group. This H2S-sensing probe was developed to differentiate tumor cells from normal cells and was tested in living cells, Caenorhabditis elegans (C. elegans), and zebrafish. The probe could successfully be used for imaging endogenous and exogenous H2S in living cells, demonstrating high sensitivity and specificity and strong anti-interference. COX2-H2S had the ability to not only discern cancer cells from normal cells but also specifically recognize 9L/lacZ cells from other glioblastoma cells (U87-MG and LN229). It could also be successfully applied for the fluorescent live imaging of H2S in both C. elegans and zebrafish.
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