Y Goa, J G Du, C Jirapattharasate, E Galon, S W Ji, Z G Ran, Y Q Xia
{"title":"一种无毒重组蛋白 rSUMO-CPBm4 可作为预防 C 型产气荚膜梭菌 beta 肠毒血症的候选疫苗。","authors":"Y Goa, J G Du, C Jirapattharasate, E Galon, S W Ji, Z G Ran, Y Q Xia","doi":"10.47665/tb.40.4.004","DOIUrl":null,"url":null,"abstract":"<p><p>Beta toxin (CPB) is a lethal toxin and plays a key role in enterotoxemia of ruminants caused by Clostridium perfringens type C strain. The existing vaccines based on crude CPB need time-consuming detoxification and difficult quality control steps. In this study, we synthesized the rCPB<sub>m4</sub> of C. perfringens type C strain and small ubiquitin-like modifier (SUMO)-tag CPB<sub>m4</sub> (rSUMO-CPB<sub>m4</sub>) by introducing four amino acid substitutions: R212E, Y266A, L268G, and W275A. Compared with rCPB<sub>m4</sub>, rSUMO-CPB<sub>m4</sub> was expressed with higher solubility in Escherichia coli BL21 (DE3). Neither rCPB<sub>m4</sub> nor rSUMO-CPB<sub>m4</sub> was lethal to mice. Although rCPB<sub>m4</sub> and rSUMO-CPB<sub>m4</sub> were reactogenic with polyclonal antibodies against crude CPB, rabbits vaccinated with rSUMO-CPB<sub>m4</sub> developed significant levels of toxin-neutralizing antibody (TNA) titers that conferred protection against crude toxin challenge. These data suggest that genetically detoxified rSUMO-CPB<sub>m4</sub> is a promising subunit vaccine candidate for C. perfringens type C beta enterotoxemia.</p>","PeriodicalId":101343,"journal":{"name":"Tropical biomedicine","volume":"40 4","pages":"400-405"},"PeriodicalIF":0.0000,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A non-toxic recombinant protein rSUMO-CPBm4 as a potential vaccine candidate against Clostridium perfringens type C beta enterotoxemia.\",\"authors\":\"Y Goa, J G Du, C Jirapattharasate, E Galon, S W Ji, Z G Ran, Y Q Xia\",\"doi\":\"10.47665/tb.40.4.004\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Beta toxin (CPB) is a lethal toxin and plays a key role in enterotoxemia of ruminants caused by Clostridium perfringens type C strain. The existing vaccines based on crude CPB need time-consuming detoxification and difficult quality control steps. In this study, we synthesized the rCPB<sub>m4</sub> of C. perfringens type C strain and small ubiquitin-like modifier (SUMO)-tag CPB<sub>m4</sub> (rSUMO-CPB<sub>m4</sub>) by introducing four amino acid substitutions: R212E, Y266A, L268G, and W275A. Compared with rCPB<sub>m4</sub>, rSUMO-CPB<sub>m4</sub> was expressed with higher solubility in Escherichia coli BL21 (DE3). Neither rCPB<sub>m4</sub> nor rSUMO-CPB<sub>m4</sub> was lethal to mice. Although rCPB<sub>m4</sub> and rSUMO-CPB<sub>m4</sub> were reactogenic with polyclonal antibodies against crude CPB, rabbits vaccinated with rSUMO-CPB<sub>m4</sub> developed significant levels of toxin-neutralizing antibody (TNA) titers that conferred protection against crude toxin challenge. These data suggest that genetically detoxified rSUMO-CPB<sub>m4</sub> is a promising subunit vaccine candidate for C. perfringens type C beta enterotoxemia.</p>\",\"PeriodicalId\":101343,\"journal\":{\"name\":\"Tropical biomedicine\",\"volume\":\"40 4\",\"pages\":\"400-405\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Tropical biomedicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.47665/tb.40.4.004\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tropical biomedicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.47665/tb.40.4.004","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
A non-toxic recombinant protein rSUMO-CPBm4 as a potential vaccine candidate against Clostridium perfringens type C beta enterotoxemia.
Beta toxin (CPB) is a lethal toxin and plays a key role in enterotoxemia of ruminants caused by Clostridium perfringens type C strain. The existing vaccines based on crude CPB need time-consuming detoxification and difficult quality control steps. In this study, we synthesized the rCPBm4 of C. perfringens type C strain and small ubiquitin-like modifier (SUMO)-tag CPBm4 (rSUMO-CPBm4) by introducing four amino acid substitutions: R212E, Y266A, L268G, and W275A. Compared with rCPBm4, rSUMO-CPBm4 was expressed with higher solubility in Escherichia coli BL21 (DE3). Neither rCPBm4 nor rSUMO-CPBm4 was lethal to mice. Although rCPBm4 and rSUMO-CPBm4 were reactogenic with polyclonal antibodies against crude CPB, rabbits vaccinated with rSUMO-CPBm4 developed significant levels of toxin-neutralizing antibody (TNA) titers that conferred protection against crude toxin challenge. These data suggest that genetically detoxified rSUMO-CPBm4 is a promising subunit vaccine candidate for C. perfringens type C beta enterotoxemia.
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