超分辨率显微镜,破译线粒体结构和动态的技术突破。

IF 6.2 2区 生物学 Q1 CELL BIOLOGY
Pauline Teixeira , Rémi Galland , Arnaud Chevrollier
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引用次数: 0

摘要

线粒体是一种复杂的细胞器,外膜包裹着第二层内膜,第二层内膜形成一个巨大的基质空间,基质空间被口袋或嵴隔开,嵴与外围内膜之间有几个薄薄的连接点。线粒体有几微米长,直径一般接近 300 纳米,膜层之间相隔几十纳米。电子显微镜的超微结构数据揭示了这些线粒体的结构,而传统的光学显微镜则通过融合、裂变和迁移过程揭示了线粒体的非凡动态,但其有限的分辨率限制了进一步研究的可能性。通过克服光衍射的限制,超分辨显微镜(SRM)现在有可能建立线粒体膜超微结构和重塑之间的联系,从而使我们对线粒体结构-功能的理解取得重大进展。在此,我们回顾了 SRM 成像对我们理解线粒体结构与功能之间关系的贡献。这些新的成像方法对线粒体病理学尤为重要,它们的前景如何?
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Super-resolution microscopies, technological breakthrough to decipher mitochondrial structure and dynamic.

Mitochondria are complex organelles with an outer membrane enveloping a second inner membrane that creates a vast matrix space partitioned by pockets or cristae that join the peripheral inner membrane with several thin junctions. Several micrometres long, mitochondria are generally close to 300 nm in diameter, with membrane layers separated by a few tens of nanometres. Ultrastructural data from electron microscopy revealed the structure of these mitochondria, while conventional optical microscopy revealed their extraordinary dynamics through fusion, fission, and migration processes but its limited resolution power restricted the possibility to go further. By overcoming the limits of light diffraction, Super-Resolution Microscopy (SRM) now offers the potential to establish the links between the ultrastructure and remodelling of mitochondrial membranes, leading to major advances in our understanding of mitochondria’s structure-function. Here we review the contributions of SRM imaging to our understanding of the relationship between mitochondrial structure and function. What are the hopes for these new imaging approaches which are particularly important for mitochondrial pathologies?

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来源期刊
CiteScore
15.10
自引率
1.40%
发文量
310
审稿时长
9.1 weeks
期刊介绍: Seminars in Cell and Developmental Biology is a review journal dedicated to keeping scientists informed of developments in the field of molecular cell and developmental biology, on a topic by topic basis. Each issue is thematic in approach, devoted to an important topic of interest to cell and developmental biologists, focusing on the latest advances and their specific implications. The aim of each issue is to provide a coordinated, readable, and lively review of a selected area, published rapidly to ensure currency.
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