Gunaseelan Aiswareya, Sanjeev K Verma, Saba Khan, Mohammad Owais, Izharul H Farooqi, Sufia Naseem
{"title":"不同正畸托槽-钢丝组合的金属释放和细胞毒性：体外研究","authors":"Gunaseelan Aiswareya, Sanjeev K Verma, Saba Khan, Mohammad Owais, Izharul H Farooqi, Sufia Naseem","doi":"10.4103/jispcd.JISPCD_65_23","DOIUrl":null,"url":null,"abstract":"<p><strong>Aim: </strong>To quantify and compare the metal ions released from different bracket-wire combinations and to assess their cytotoxicity.</p><p><strong>Materials and methods: </strong>A total of 360 fabricated sectional fixed orthodontic appliances were divided into 6 groups. The first three groups consisted of stainless-steel brackets with stainless-steel, snickel-titanium (NiTi), and titanium-molybdenum alloy (TMA) archwires, and the other three groups were fabricated using ceramic brackets (polycrystalline alumina) with stainless-steel, NiTi, and TMA archwires. These appliances were immersed in artificial saliva (pH 6.5 ± 0.5, 37°C), for 1 week, 2 weeks, and 1 month. The nickel and chromium ions released in the artificial saliva were quantified using a flame atomic absorption spectrometer, and cytotoxicity assessment was performed using a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay on human cervical cancer cell line.</p><p><strong>Results: </strong>The stainless-steel bracket groups displayed a significantly greater release of nickel and chromium ions compared to the ceramic bracket groups (<i>P</i> < 0.05). However, no significant differences were identified when comparing the three archwire types within the stainless-steel/ceramic bracket groups. At the end of 1 month, the % cell viability demonstrated by the appliances was in the decreasing order of stainless-steel-TMA > ceramic-stainless steel > stainless-steel-NiTi > ceramic-NiTi > stainless-steel-stainless steel > ceramic-TMA.</p><p><strong>Conclusion: </strong>Considerably greater release of nickel and chromium ions was observed from the appliances utilizing stainless-steel brackets compared to those employing ceramic brackets. However, no remarkable difference in the levels of nickel and chromium ions was observed when comparing the three archwires: stainless steel, NiTi, and TMA. In the cytotoxicity assessment, the ceramic-TMA combination displayed the highest level of cytotoxicity, while the stainless-steel-TMA combination displayed the least cytotoxicity.</p>","PeriodicalId":47247,"journal":{"name":"Journal of International Society of Preventive and Community Dentistry","volume":"13 6","pages":"469-476"},"PeriodicalIF":1.4000,"publicationDate":"2023-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10829279/pdf/","citationCount":"0","resultStr":"{\"title\":\"Metal Release and Cytotoxicity of Different Orthodontic Bracket-Wire Combinations: An <i>In Vitro</i> Study.\",\"authors\":\"Gunaseelan Aiswareya, Sanjeev K Verma, Saba Khan, Mohammad Owais, Izharul H Farooqi, Sufia Naseem\",\"doi\":\"10.4103/jispcd.JISPCD_65_23\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Aim: </strong>To quantify and compare the metal ions released from different bracket-wire combinations and to assess their cytotoxicity.</p><p><strong>Materials and methods: </strong>A total of 360 fabricated sectional fixed orthodontic appliances were divided into 6 groups. The first three groups consisted of stainless-steel brackets with stainless-steel, snickel-titanium (NiTi), and titanium-molybdenum alloy (TMA) archwires, and the other three groups were fabricated using ceramic brackets (polycrystalline alumina) with stainless-steel, NiTi, and TMA archwires. These appliances were immersed in artificial saliva (pH 6.5 ± 0.5, 37°C), for 1 week, 2 weeks, and 1 month. The nickel and chromium ions released in the artificial saliva were quantified using a flame atomic absorption spectrometer, and cytotoxicity assessment was performed using a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay on human cervical cancer cell line.</p><p><strong>Results: </strong>The stainless-steel bracket groups displayed a significantly greater release of nickel and chromium ions compared to the ceramic bracket groups (<i>P</i> < 0.05). However, no significant differences were identified when comparing the three archwire types within the stainless-steel/ceramic bracket groups. At the end of 1 month, the % cell viability demonstrated by the appliances was in the decreasing order of stainless-steel-TMA > ceramic-stainless steel > stainless-steel-NiTi > ceramic-NiTi > stainless-steel-stainless steel > ceramic-TMA.</p><p><strong>Conclusion: </strong>Considerably greater release of nickel and chromium ions was observed from the appliances utilizing stainless-steel brackets compared to those employing ceramic brackets. However, no remarkable difference in the levels of nickel and chromium ions was observed when comparing the three archwires: stainless steel, NiTi, and TMA. In the cytotoxicity assessment, the ceramic-TMA combination displayed the highest level of cytotoxicity, while the stainless-steel-TMA combination displayed the least cytotoxicity.</p>\",\"PeriodicalId\":47247,\"journal\":{\"name\":\"Journal of International Society of Preventive and Community Dentistry\",\"volume\":\"13 6\",\"pages\":\"469-476\"},\"PeriodicalIF\":1.4000,\"publicationDate\":\"2023-12-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10829279/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of International Society of Preventive and Community Dentistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4103/jispcd.JISPCD_65_23\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2023/11/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q3\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of International Society of Preventive and Community Dentistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4103/jispcd.JISPCD_65_23","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/11/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
Metal Release and Cytotoxicity of Different Orthodontic Bracket-Wire Combinations: An In Vitro Study.
Aim: To quantify and compare the metal ions released from different bracket-wire combinations and to assess their cytotoxicity.
Materials and methods: A total of 360 fabricated sectional fixed orthodontic appliances were divided into 6 groups. The first three groups consisted of stainless-steel brackets with stainless-steel, snickel-titanium (NiTi), and titanium-molybdenum alloy (TMA) archwires, and the other three groups were fabricated using ceramic brackets (polycrystalline alumina) with stainless-steel, NiTi, and TMA archwires. These appliances were immersed in artificial saliva (pH 6.5 ± 0.5, 37°C), for 1 week, 2 weeks, and 1 month. The nickel and chromium ions released in the artificial saliva were quantified using a flame atomic absorption spectrometer, and cytotoxicity assessment was performed using a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay on human cervical cancer cell line.
Results: The stainless-steel bracket groups displayed a significantly greater release of nickel and chromium ions compared to the ceramic bracket groups (P < 0.05). However, no significant differences were identified when comparing the three archwire types within the stainless-steel/ceramic bracket groups. At the end of 1 month, the % cell viability demonstrated by the appliances was in the decreasing order of stainless-steel-TMA > ceramic-stainless steel > stainless-steel-NiTi > ceramic-NiTi > stainless-steel-stainless steel > ceramic-TMA.
Conclusion: Considerably greater release of nickel and chromium ions was observed from the appliances utilizing stainless-steel brackets compared to those employing ceramic brackets. However, no remarkable difference in the levels of nickel and chromium ions was observed when comparing the three archwires: stainless steel, NiTi, and TMA. In the cytotoxicity assessment, the ceramic-TMA combination displayed the highest level of cytotoxicity, while the stainless-steel-TMA combination displayed the least cytotoxicity.
期刊介绍：
It is a journal aimed for research, scientific facts and details covering all specialties of dentistry with a good determination for exploring and sharing the knowledge in the medical and dental fraternity. The scope is therefore huge covering almost all streams of dentistry - starting from original studies, systematic reviews, narrative reviews, very unique case reports. Journal scope is not limited to these subjects and is more wider covering all specialities of dentistry follows: -Preventive and Community dentistry (Dental public health)- Endodontics- Oral and maxillofacial pathology- Oral and maxillofacial radiology- Oral and maxillofacial surgery (also called oral surgery)- Orthodontics and dentofacial orthopedics- Periodontology (also called periodontics)- Pediatric dentistry (also called pedodontics)- Prosthodontics (also called prosthetic dentistry)- Oral medicine- Special needs dentistry (also called special care dentistry)- Oral Biology- Forensic odontology- Geriatric dentistry or Geriodontics- Preventive and Social Medicine (Public health)- Our journal appreciates research articles pertaining with advancement of dentistry, preventive and community dentistry including oral epidemiology, oral health services research, oral health education and promotion, behavioral sciences related to dentistry, dental jurisprudence, ethics and oral health, economics, and quality assessment, recent advances in preventive dentistry and community dentistry.
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