紫外线 C 作为面部假体所用医用硅胶的消毒方法：体外研究 - 第二部分。

IF 4.3 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Prosthetic Dentistry Pub Date : 2024-10-01 DOI:10.1016/j.prosdent.2024.01.005

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However, whether disinfection with ultraviolet C is an effective disinfection method is unclear.</span></div></div><div><h3>Purpose</h3><div>The purpose of this in vitro study was to evaluate the effectiveness of irradiation with different exposure durations of an ultraviolet-C light-emitting diode in the disinfection of the silicone (A-588–1; Factor II) used in facial prostheses.</div></div><div><h3>Material and methods</h3><div>A total of 216 specimens were prepared, contaminated by multispecies biofilm, and divided into 9 groups (n=24) for different treatments: chlorhexidine<span><span> 0.12% (G CHG), ultraviolet-C light-emitting diode for 5 minutes (G UVC5), ultraviolet-C light-emitting diode for 10 minutes (G UVC10), ultraviolet-C light-emitting diode for 20 minutes (G UVC20), their respective untreated controls (Gcontrol CHG, Gcontrol UVC5, Gcontrol UVC10, Gcontrol UVC20), and dimethyl sulfoxide<span> (G DMSO) as the negative control. 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引用次数: 0

摘要

问题陈述：消毒是保存面部假体和保持组织健康的一个重要因素。目的：本体外研究的目的是评估不同照射时间的紫外线 C 发光二极管对面部假体中使用的硅胶（A-588-1；因子 II）的消毒效果：共制备了 216 个被多菌种生物膜污染的标本，并将其分为 9 组（n=24），分别进行不同的处理：洗必泰 0.12%（G CHG）、紫外-C 发光二极管 5 分钟（G UVC5）、紫外-C 发光二极管 10 分钟（G UVC10）、紫外-C 发光二极管 20 分钟（G UVC20）、各自的未处理对照组（Gcontrol CHG、Gcontrol UVC5、Gcontrol UVC10、Gcontrol UVC20）和作为阴性对照的二甲基亚砜（G DMSO）。细胞活力用甲基四氮唑盐（MTT）法测定。进行了两项统计分析。首先，采用 2×3 方差分析比较对照组（Gcontrol UVC5、Gcontrol UVC10 和 Gcontrol UVC20）和不同照射时间的 UV-C LED 光实验组（G UVC5、G UVC10 和 G UVC20）。第二项分析使用广义线性模型比较各组（G UVC5、G UVC10、G UVC20、G CHG 和 G DMSO）的光密度：结果：细胞存活率结果表明，与未处理的对照组相比，暴露于紫外线-C 发光二极管 20 分钟（G UVC20）后微生物数量减少（P.05）。20 分钟照射的平均光密度值最低，与 5 分钟照射的平均光密度值有统计学差异（P.05）：用紫外线-C 发光二极管照射 20 分钟可降低面部假体中使用的医用硅胶上的体外微生物细胞活力。

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Ultraviolet C as a method of disinfecting medical silicone used in facial prostheses: An in vitro study – Part 2

Statement of problem

Disinfection is an important factor in preserving facial prostheses and maintaining tissue health. However, whether disinfection with ultraviolet C is an effective disinfection method is unclear.

Purpose

The purpose of this in vitro study was to evaluate the effectiveness of irradiation with different exposure durations of an ultraviolet-C light-emitting diode in the disinfection of the silicone (A-588–1; Factor II) used in facial prostheses.

Material and methods

A total of 216 specimens were prepared, contaminated by multispecies biofilm, and divided into 9 groups (n=24) for different treatments: chlorhexidine 0.12% (G CHG), ultraviolet-C light-emitting diode for 5 minutes (G UVC5), ultraviolet-C light-emitting diode for 10 minutes (G UVC10), ultraviolet-C light-emitting diode for 20 minutes (G UVC20), their respective untreated controls (Gcontrol CHG, Gcontrol UVC5, Gcontrol UVC10, Gcontrol UVC20), and dimethyl sulfoxide (G DMSO) as the negative control. Cell viability was measured by using the methyl tetrazolium salt (MTT) method. Two statistical analyses were performed. First, a 2×3 ANOVA was carried out to compare the control groups (Gcontrol UVC5, Gcontrol UVC10, and Gcontrol UVC20) and the experimental groups of UV-C LED light with different exposure durations (G UVC5, G UVC10, and G UVC20). The second analysis was performed using generalized linear models to compare the optical density of the groups (G UVC5, G UVC10, G UVC20, G CHG, and G DMSO).

Results

Cell viability results demonstrated a microbial reduction after exposure to the ultraviolet-C light-emitting diode for 20 minutes (G UVC20) compared with untreated controls (P<.05). The 5- and 10-minute exposures were statistically similar to their respective control groups (P>.05). The 20 minutes exposure had the lowest average optical density value, being statistically different from the 5-minute exposure (P<.05). A 20-minute exposure to the ultraviolet-C light-emitting diode (G UVC20) was similarly effective when compared with the standard disinfection treatment (G CHG) and dimethyl sulfoxide (G DMSO) (P>.05).

Conclusions

Irradiation with an ultraviolet-C light-emitting diode for 20 minutes decreased the in vitro microbial cell viability on the medical silicone used in facial prostheses.

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来源期刊

Journal of Prosthetic Dentistry 医学-牙科与口腔外科

CiteScore

7.00

自引率

13.00%

发文量

599

审稿时长

69 days

期刊介绍： The Journal of Prosthetic Dentistry is the leading professional journal devoted exclusively to prosthetic and restorative dentistry. The Journal is the official publication for 24 leading U.S. international prosthodontic organizations. The monthly publication features timely, original peer-reviewed articles on the newest techniques, dental materials, and research findings. The Journal serves prosthodontists and dentists in advanced practice, and features color photos that illustrate many step-by-step procedures. The Journal of Prosthetic Dentistry is included in Index Medicus and CINAHL.