在使用 RPTEC/TERT1 细胞系的肾脏纤维化体外模型中,BMSC 调节培养基的治疗潜力。

IF 2.9 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
BMB Reports Pub Date : 2024-02-01
Yunji Kim, Dayeon Kang, Ga-Eun Choi, Sang Dae Kim, Sun-Ja Yang, Hyosang Kim, Dalsan You, Choung Soo Kim, Nayoung Suh
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引用次数: 0

摘要

我们研究了骨髓间充质干细胞调节培养基(BMSC-CM)在纤维化环境中对永生化肾近曲小管上皮细胞(RPTEC/ TERT1)的治疗潜力。为了复制慢性肾病患者肾脏特有的硬度增加现象,我们使用了聚丙烯酰胺凝胶平台。结果表明,僵硬的基质会增加α-平滑肌肌动蛋白(α-SMA)的水平,这表明RPTEC/TERT1细胞发生了纤维化活化。有趣的是,用 BMSC-CM 处理后,纤维化标记物(α-SMA 和波形蛋白)的水平明显降低,而上皮标记物 E-cadherin 和 aquaporin 7 的水平则升高,尤其是在僵硬条件下。此外,BMSC-CM 还能改变微核糖核酸(miRNA)的表达,降低这些细胞的氧化应激水平。我们的研究结果表明,BMSC-CM 可调节 RPTEC/TERT1 细胞的细胞形态、miRNA 表达和氧化应激,突出了其在纤维化肾病中的治疗潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Therapeutic potential of BMSC-conditioned medium in an in vitro model of renal fibrosis using the RPTEC/TERT1 cell line.

We investigated the therapeutic potential of bone marrow-derived mesenchymal stem cell-conditioned medium (BMSC-CM) on immortalized renal proximal tubule epithelial cells (RPTEC/ TERT1) in a fibrotic environment. To replicate the increased stiffness characteristic of kidneys in chronic kidney disease, we utilized polyacrylamide gel platforms. A stiff matrix was shown to increase α-smooth muscle actin (α-SMA) levels, indicating fibrogenic activation in RPTEC/TERT1 cells. Interestingly, treatment with BMSC-CM resulted in significant reductions in the levels of fibrotic markers (α-SMA and vimentin) and increases in the levels of the epithelial marker E-cadherin and aquaporin 7, particularly under stiff conditions. Furthermore, BMSC-CM modified microRNA (miRNA) expression and reduced oxidative stress levels in these cells. Our findings suggest that BMSC-CM can modulate cellular morphology, miRNA expression, and oxidative stress in RPTEC/TERT1 cells, highlighting its therapeutic potential in fibrotic kidney disease. [BMB Reports 2024; 57(2): 116-121].

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来源期刊
BMB Reports
BMB Reports 生物-生化与分子生物学
CiteScore
5.10
自引率
7.90%
发文量
141
审稿时长
1 months
期刊介绍: The BMB Reports (BMB Rep, established in 1968) is published at the end of every month by Korean Society for Biochemistry and Molecular Biology. Copyright is reserved by the Society. The journal publishes short articles and mini reviews. We expect that the BMB Reports will deliver the new scientific findings and knowledge to our readers in fast and timely manner.
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