A. Babayan , A. Vassilian , A. Poladyan , K. Trchounian
{"title":"大肠杆菌 FocA 和 FocB 甲酸盐通道在能量受限的静止期发酵细胞渗透压胁迫期间控制质子/钾通量和氢气产生的作用","authors":"A. Babayan , A. Vassilian , A. Poladyan , K. Trchounian","doi":"10.1016/j.biochi.2024.01.017","DOIUrl":null,"url":null,"abstract":"<div><p><em>Escherichia coli</em> FocA and FocB formate channels export formate or import it for further disproportionation by the formate hydrogenlyase (FHL) complex to H<sub>2</sub> and CO<sub>2</sub>. Here, we show that under pH and osmotic stress FocA and FocB play important roles in regulating proton and potassium fluxes and couple this with H<sub>2</sub> production in stationary-phase cells. Using whole-cell assays with glucose as electron donor, a <em>focB</em> mutant showed a 50 % decrease in V<sub>H2</sub>, while <em>N’N’</em>-dicyclohexylcarbodiimide (DCCD) treatment of osmotically stressed cells underlined the role of F<sub>O</sub>F<sub>1</sub> ATPase in H<sub>2</sub> production. At pH 7.5 and under osmotic stress FocB contributed to the proton flux but not to the potassium flux. At pH 5.5 both formate channels contributed to the proton and potassium fluxes. Particulalry, a <em>focA</em> mutant had 40 % lower potassium flux whereas the proton flux increased approximately two-fold. Moreover, at pH 5.5H<sub>2</sub> production was totally inhibited by DCCD in the <em>focA</em> mutant. Taken together, our results suggest that depending on external pH, the formate channels play an important role in osmoregulation by helping to balance proton/potassium fluxes and H<sub>2</sub> production, and thus assist the proton F<sub>O</sub>F<sub>1</sub>-ATPase in maintenance of ion gradients in fermenting stationary-phase cells.</p></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"221 ","pages":"Pages 91-98"},"PeriodicalIF":3.3000,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Role of the Escherichia coli FocA and FocB formate channels in controlling proton/potassium fluxes and hydrogen production during osmotic stress in energy-limited, stationary phase fermenting cells\",\"authors\":\"A. Babayan , A. Vassilian , A. Poladyan , K. Trchounian\",\"doi\":\"10.1016/j.biochi.2024.01.017\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><em>Escherichia coli</em> FocA and FocB formate channels export formate or import it for further disproportionation by the formate hydrogenlyase (FHL) complex to H<sub>2</sub> and CO<sub>2</sub>. Here, we show that under pH and osmotic stress FocA and FocB play important roles in regulating proton and potassium fluxes and couple this with H<sub>2</sub> production in stationary-phase cells. Using whole-cell assays with glucose as electron donor, a <em>focB</em> mutant showed a 50 % decrease in V<sub>H2</sub>, while <em>N’N’</em>-dicyclohexylcarbodiimide (DCCD) treatment of osmotically stressed cells underlined the role of F<sub>O</sub>F<sub>1</sub> ATPase in H<sub>2</sub> production. At pH 7.5 and under osmotic stress FocB contributed to the proton flux but not to the potassium flux. At pH 5.5 both formate channels contributed to the proton and potassium fluxes. Particulalry, a <em>focA</em> mutant had 40 % lower potassium flux whereas the proton flux increased approximately two-fold. Moreover, at pH 5.5H<sub>2</sub> production was totally inhibited by DCCD in the <em>focA</em> mutant. Taken together, our results suggest that depending on external pH, the formate channels play an important role in osmoregulation by helping to balance proton/potassium fluxes and H<sub>2</sub> production, and thus assist the proton F<sub>O</sub>F<sub>1</sub>-ATPase in maintenance of ion gradients in fermenting stationary-phase cells.</p></div>\",\"PeriodicalId\":251,\"journal\":{\"name\":\"Biochimie\",\"volume\":\"221 \",\"pages\":\"Pages 91-98\"},\"PeriodicalIF\":3.3000,\"publicationDate\":\"2024-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimie\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S030090842400035X\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimie","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S030090842400035X","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Role of the Escherichia coli FocA and FocB formate channels in controlling proton/potassium fluxes and hydrogen production during osmotic stress in energy-limited, stationary phase fermenting cells
Escherichia coli FocA and FocB formate channels export formate or import it for further disproportionation by the formate hydrogenlyase (FHL) complex to H2 and CO2. Here, we show that under pH and osmotic stress FocA and FocB play important roles in regulating proton and potassium fluxes and couple this with H2 production in stationary-phase cells. Using whole-cell assays with glucose as electron donor, a focB mutant showed a 50 % decrease in VH2, while N’N’-dicyclohexylcarbodiimide (DCCD) treatment of osmotically stressed cells underlined the role of FOF1 ATPase in H2 production. At pH 7.5 and under osmotic stress FocB contributed to the proton flux but not to the potassium flux. At pH 5.5 both formate channels contributed to the proton and potassium fluxes. Particulalry, a focA mutant had 40 % lower potassium flux whereas the proton flux increased approximately two-fold. Moreover, at pH 5.5H2 production was totally inhibited by DCCD in the focA mutant. Taken together, our results suggest that depending on external pH, the formate channels play an important role in osmoregulation by helping to balance proton/potassium fluxes and H2 production, and thus assist the proton FOF1-ATPase in maintenance of ion gradients in fermenting stationary-phase cells.
期刊介绍:
Biochimie publishes original research articles, short communications, review articles, graphical reviews, mini-reviews, and hypotheses in the broad areas of biology, including biochemistry, enzymology, molecular and cell biology, metabolic regulation, genetics, immunology, microbiology, structural biology, genomics, proteomics, and molecular mechanisms of disease. Biochimie publishes exclusively in English.
Articles are subject to peer review, and must satisfy the requirements of originality, high scientific integrity and general interest to a broad range of readers. Submissions that are judged to be of sound scientific and technical quality but do not fully satisfy the requirements for publication in Biochimie may benefit from a transfer service to a more suitable journal within the same subject area.