In vitro callus induction and growth for medicinal use of an endangered Korean native plant, Glochidion chodoense C. S. Lee & H. T. Im

Glochidion chodoense C. S. Lee & H. T. Im is an endangered plant found in Korea. Its conservation and potential industrial utilization require the development of suitable genetic resource propagation approaches. We here present a protocol for the efficient propagation of G. chodoense leaf segments by callus induction. We assessed various induction media containing different concentrations of plant growth regulators, as well as antioxidants to inhibit callus browning. The use of MS medium with 2,4-Di-chlorophenoxy-acetic acid (2,4-D) (1 mg/L) and BA (0.5 mg/L) for callus induction and incorporating ascorbic acid (10 mg/L) to mitigate browning was found to offer a promising approach for the mass production of G. chodoense. This protocol has significant potential for the conservation and industrial utilization of this endangered species.