在促进植物生长的多物种生物膜中,种间相互作用有利于关键物种的生长

Nan Yang, Henriette L Røder, Wisnu Adi Wicaksono, Birgit Wassermann, Jakob Russel, Xuanji Li, Joseph Nesme, Gabriele Berg, Søren J Sørensen, Mette Burmølle
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引用次数: 0

摘要

定殖于植物根部的微生物共存于复杂的多物种生物膜群落中。然而,人们对建立在植物根部的生物膜群落内的微生物相互作用和基本空间组织知之甚少。在此,我们将一个成熟的四种生物膜模型(根瘤黑僵菌、淀粉样芽孢杆菌、氧单胞菌和黄单胞菌,简称 SPMX)应用于拟南芥根部,研究多物种生物膜对植物生长和根部群落空间动态的影响。SPMX共培养显著促进了根系发育和植物生物量。共培养的 SPMX 增加了根部定殖,并形成了多物种生物膜,在结构上不同于单培养形成的生物膜。通过结合 16S rRNA 基因扩增片段测序和荧光原位杂交与共聚焦激光扫描显微镜(FISH-CLSM),我们发现四种生物膜的组成和空间组织随着时间的推移发生了显著变化。单培养的溶淀粉镰刀菌在植物根部的定殖能力很差,但当其驻留在四种生物膜中时,其种群数量和根部定殖能力都大大增强。将淀粉样溶菌排除在群落之外会降低生物膜的总体产量和三种菌的根定植率,从而失去促进植物生长的作用。结合空间分析,我们发现淀粉菌是一个关键物种。我们的研究结果突出表明,弱根定植者可能会从复杂群落中的互利相互作用中获益,从而成为影响群落空间组织和功能的重要基石物种。这项工作拓展了有关空间组织的知识,揭示了植物根部多物种生物膜群落中的种间相互作用,有利于利用微生物的互利性促进植物生长。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Interspecific interactions facilitate keystone species in a multispecies biofilm that promotes plant growth
Microorganisms colonizing plant roots co-exist in complex, spatially structured multispecies biofilm communities. However, little is known about microbial interactions and the underlying spatial organization within biofilm communities established on plant roots. Here, a well-established four-species biofilm model (Stenotrophomonas rhizophila, Paenibacillus amylolyticus, Microbacterium oxydans and Xanthomonas retroflexus, termed as SPMX) was applied to Arabidopsis roots to study the impact of multispecies biofilm on plant growth and the community spatial dynamics on the roots. SPMX co-culture notably promoted root development and plant biomass. Co-cultured SPMX increased root colonization and formed multispecies biofilms, structurally different from those formed by monocultures. By combining 16S rRNA gene amplicon sequencing and fluorescence in situ hybridization with confocal laser scanning microscopy (FISH-CLSM), we found that the composition and spatial organization of the four-species biofilm significantly changed over time. Monoculture P. amylolyticus colonized plant roots poorly, but its population and root colonization were highly enhanced when residing in the four-species biofilm. Exclusion of P. amylolyticus from the community reduced overall biofilm production and root colonization of the three species, resulting in the loss of the plant growth-promoting effects. Combined with spatial analysis, this led to identification of P. amylolyticus as a keystone species. Our findings highlight that weak root colonizers may benefit from mutualistic interactions in complex communities and hereby become important keystone species impacting community spatial organization and function. This work expands the knowledge on spatial organization uncovering interspecific interactions in multispecies biofilm communities on plant roots, beneficial for harnessing microbial mutualism promoting plant growth.
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