Long Zhang, Zhiran Zhou, Fan Cai, Wanli Qin, Li Liu, Guli Kuerban, Lin Jin, Xiaoli Ma
{"title":"硫酸酯酶修饰因子 1(HaSumf1)在 Helicoverpa armigera 的发育和对宿主葡萄糖苷酸的适应中的功能","authors":"Long Zhang, Zhiran Zhou, Fan Cai, Wanli Qin, Li Liu, Guli Kuerban, Lin Jin, Xiaoli Ma","doi":"10.1111/jen.13236","DOIUrl":null,"url":null,"abstract":"<p>The system of gulcosinoalte sulfatase–sulfatase-modifying factors (Sumfs) in herbivorous insects fulfils an essential function in defending host plant glucosinolates (GLSs)—the compounds of cruciferous plants—by swiftly desulfurising them. Previously, eight HaSulfs and one HaSumf1 were identified in <i>Helicoverpa armigera</i>. GLSs have a significant impact on the growth and development of <i>H. armigera</i> larvae. They can induce the expression of <i>HaSulf</i> in the midgut of <i>H. armigera</i> at both gene and protein levels. In the present study, we utilised RNAi technology to silence the <i>HaSumf1</i> gene and explored its potential impact on the growth, development, and adaptation of GLSs. The sequence analysis has indicated that the HaSumf1 gene in <i>H. armigera</i> shares a high degree of sequence identity with other insect genes and is expressed at its highest level in the fifth-instar phase. Additionally, the qRT-PCR results reveal that the expression of <i>HaSumf1</i> was upregulated by 1.26 and 0.07 times in third-instar and 1.65 and 0.19 times in fifth-instar when fed with 0.004% and 0.04% GLS, respectively. The effectiveness of dsSumf1 silencing was observed in both third- and fifth-instar stages, with silencing rates of 65.5%–88.9% and 61.3%–74.6%, respectively (<i>p</i> < 0.05). The findings of the bioassay indicate that <i>H. armigera's</i> body weight at fifth-instar significantly increased after treatment with 0.004% GLS and 0.04% GLS, while the larval survival rate, pupation rate, and pupa weight decreased. Notably, when feeding dsRNA-encapsulated carbon quantum dots (CQD-dsRNA), this trend was significantly strengthened (<i>p</i> < 0.05). Taken together, these findings provide evidence supporting HaSumf1's conservation involvement in the adaptation of host plant GLSs during lepidopteran insect evolution. Therefore, it plays a critical role in the growth and development of <i>H. armigera</i>, offering molecular targets for developing novel pesticides and targeted control measures against this vital pest.</p>","PeriodicalId":14987,"journal":{"name":"Journal of Applied Entomology","volume":null,"pages":null},"PeriodicalIF":1.7000,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Functions of the sulfatase-modifying factor 1 (HaSumf1) in the development and host glucosinolate adaptation of Helicoverpa armigera\",\"authors\":\"Long Zhang, Zhiran Zhou, Fan Cai, Wanli Qin, Li Liu, Guli Kuerban, Lin Jin, Xiaoli Ma\",\"doi\":\"10.1111/jen.13236\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The system of gulcosinoalte sulfatase–sulfatase-modifying factors (Sumfs) in herbivorous insects fulfils an essential function in defending host plant glucosinolates (GLSs)—the compounds of cruciferous plants—by swiftly desulfurising them. Previously, eight HaSulfs and one HaSumf1 were identified in <i>Helicoverpa armigera</i>. GLSs have a significant impact on the growth and development of <i>H. armigera</i> larvae. They can induce the expression of <i>HaSulf</i> in the midgut of <i>H. armigera</i> at both gene and protein levels. In the present study, we utilised RNAi technology to silence the <i>HaSumf1</i> gene and explored its potential impact on the growth, development, and adaptation of GLSs. The sequence analysis has indicated that the HaSumf1 gene in <i>H. armigera</i> shares a high degree of sequence identity with other insect genes and is expressed at its highest level in the fifth-instar phase. Additionally, the qRT-PCR results reveal that the expression of <i>HaSumf1</i> was upregulated by 1.26 and 0.07 times in third-instar and 1.65 and 0.19 times in fifth-instar when fed with 0.004% and 0.04% GLS, respectively. The effectiveness of dsSumf1 silencing was observed in both third- and fifth-instar stages, with silencing rates of 65.5%–88.9% and 61.3%–74.6%, respectively (<i>p</i> < 0.05). The findings of the bioassay indicate that <i>H. armigera's</i> body weight at fifth-instar significantly increased after treatment with 0.004% GLS and 0.04% GLS, while the larval survival rate, pupation rate, and pupa weight decreased. Notably, when feeding dsRNA-encapsulated carbon quantum dots (CQD-dsRNA), this trend was significantly strengthened (<i>p</i> < 0.05). Taken together, these findings provide evidence supporting HaSumf1's conservation involvement in the adaptation of host plant GLSs during lepidopteran insect evolution. Therefore, it plays a critical role in the growth and development of <i>H. armigera</i>, offering molecular targets for developing novel pesticides and targeted control measures against this vital pest.</p>\",\"PeriodicalId\":14987,\"journal\":{\"name\":\"Journal of Applied Entomology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2024-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Applied Entomology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/jen.13236\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENTOMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Applied Entomology","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jen.13236","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
Functions of the sulfatase-modifying factor 1 (HaSumf1) in the development and host glucosinolate adaptation of Helicoverpa armigera
The system of gulcosinoalte sulfatase–sulfatase-modifying factors (Sumfs) in herbivorous insects fulfils an essential function in defending host plant glucosinolates (GLSs)—the compounds of cruciferous plants—by swiftly desulfurising them. Previously, eight HaSulfs and one HaSumf1 were identified in Helicoverpa armigera. GLSs have a significant impact on the growth and development of H. armigera larvae. They can induce the expression of HaSulf in the midgut of H. armigera at both gene and protein levels. In the present study, we utilised RNAi technology to silence the HaSumf1 gene and explored its potential impact on the growth, development, and adaptation of GLSs. The sequence analysis has indicated that the HaSumf1 gene in H. armigera shares a high degree of sequence identity with other insect genes and is expressed at its highest level in the fifth-instar phase. Additionally, the qRT-PCR results reveal that the expression of HaSumf1 was upregulated by 1.26 and 0.07 times in third-instar and 1.65 and 0.19 times in fifth-instar when fed with 0.004% and 0.04% GLS, respectively. The effectiveness of dsSumf1 silencing was observed in both third- and fifth-instar stages, with silencing rates of 65.5%–88.9% and 61.3%–74.6%, respectively (p < 0.05). The findings of the bioassay indicate that H. armigera's body weight at fifth-instar significantly increased after treatment with 0.004% GLS and 0.04% GLS, while the larval survival rate, pupation rate, and pupa weight decreased. Notably, when feeding dsRNA-encapsulated carbon quantum dots (CQD-dsRNA), this trend was significantly strengthened (p < 0.05). Taken together, these findings provide evidence supporting HaSumf1's conservation involvement in the adaptation of host plant GLSs during lepidopteran insect evolution. Therefore, it plays a critical role in the growth and development of H. armigera, offering molecular targets for developing novel pesticides and targeted control measures against this vital pest.
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The Journal of Applied Entomology publishes original articles on current research in applied entomology, including mites and spiders in terrestrial ecosystems.
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