硫酸酯酶修饰因子 1(HaSumf1)在 Helicoverpa armigera 的发育和对宿主葡萄糖苷酸的适应中的功能

IF 1.7 3区 农林科学 Q2 ENTOMOLOGY
Long Zhang, Zhiran Zhou, Fan Cai, Wanli Qin, Li Liu, Guli Kuerban, Lin Jin, Xiaoli Ma
{"title":"硫酸酯酶修饰因子 1(HaSumf1)在 Helicoverpa armigera 的发育和对宿主葡萄糖苷酸的适应中的功能","authors":"Long Zhang,&nbsp;Zhiran Zhou,&nbsp;Fan Cai,&nbsp;Wanli Qin,&nbsp;Li Liu,&nbsp;Guli Kuerban,&nbsp;Lin Jin,&nbsp;Xiaoli Ma","doi":"10.1111/jen.13236","DOIUrl":null,"url":null,"abstract":"<p>The system of gulcosinoalte sulfatase–sulfatase-modifying factors (Sumfs) in herbivorous insects fulfils an essential function in defending host plant glucosinolates (GLSs)—the compounds of cruciferous plants—by swiftly desulfurising them. Previously, eight HaSulfs and one HaSumf1 were identified in <i>Helicoverpa armigera</i>. GLSs have a significant impact on the growth and development of <i>H. armigera</i> larvae. They can induce the expression of <i>HaSulf</i> in the midgut of <i>H. armigera</i> at both gene and protein levels. In the present study, we utilised RNAi technology to silence the <i>HaSumf1</i> gene and explored its potential impact on the growth, development, and adaptation of GLSs. The sequence analysis has indicated that the HaSumf1 gene in <i>H. armigera</i> shares a high degree of sequence identity with other insect genes and is expressed at its highest level in the fifth-instar phase. Additionally, the qRT-PCR results reveal that the expression of <i>HaSumf1</i> was upregulated by 1.26 and 0.07 times in third-instar and 1.65 and 0.19 times in fifth-instar when fed with 0.004% and 0.04% GLS, respectively. The effectiveness of dsSumf1 silencing was observed in both third- and fifth-instar stages, with silencing rates of 65.5%–88.9% and 61.3%–74.6%, respectively (<i>p</i> &lt; 0.05). The findings of the bioassay indicate that <i>H. armigera's</i> body weight at fifth-instar significantly increased after treatment with 0.004% GLS and 0.04% GLS, while the larval survival rate, pupation rate, and pupa weight decreased. Notably, when feeding dsRNA-encapsulated carbon quantum dots (CQD-dsRNA), this trend was significantly strengthened (<i>p</i> &lt; 0.05). Taken together, these findings provide evidence supporting HaSumf1's conservation involvement in the adaptation of host plant GLSs during lepidopteran insect evolution. Therefore, it plays a critical role in the growth and development of <i>H. armigera</i>, offering molecular targets for developing novel pesticides and targeted control measures against this vital pest.</p>","PeriodicalId":14987,"journal":{"name":"Journal of Applied Entomology","volume":null,"pages":null},"PeriodicalIF":1.7000,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Functions of the sulfatase-modifying factor 1 (HaSumf1) in the development and host glucosinolate adaptation of Helicoverpa armigera\",\"authors\":\"Long Zhang,&nbsp;Zhiran Zhou,&nbsp;Fan Cai,&nbsp;Wanli Qin,&nbsp;Li Liu,&nbsp;Guli Kuerban,&nbsp;Lin Jin,&nbsp;Xiaoli Ma\",\"doi\":\"10.1111/jen.13236\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The system of gulcosinoalte sulfatase–sulfatase-modifying factors (Sumfs) in herbivorous insects fulfils an essential function in defending host plant glucosinolates (GLSs)—the compounds of cruciferous plants—by swiftly desulfurising them. Previously, eight HaSulfs and one HaSumf1 were identified in <i>Helicoverpa armigera</i>. GLSs have a significant impact on the growth and development of <i>H. armigera</i> larvae. They can induce the expression of <i>HaSulf</i> in the midgut of <i>H. armigera</i> at both gene and protein levels. In the present study, we utilised RNAi technology to silence the <i>HaSumf1</i> gene and explored its potential impact on the growth, development, and adaptation of GLSs. The sequence analysis has indicated that the HaSumf1 gene in <i>H. armigera</i> shares a high degree of sequence identity with other insect genes and is expressed at its highest level in the fifth-instar phase. Additionally, the qRT-PCR results reveal that the expression of <i>HaSumf1</i> was upregulated by 1.26 and 0.07 times in third-instar and 1.65 and 0.19 times in fifth-instar when fed with 0.004% and 0.04% GLS, respectively. The effectiveness of dsSumf1 silencing was observed in both third- and fifth-instar stages, with silencing rates of 65.5%–88.9% and 61.3%–74.6%, respectively (<i>p</i> &lt; 0.05). The findings of the bioassay indicate that <i>H. armigera's</i> body weight at fifth-instar significantly increased after treatment with 0.004% GLS and 0.04% GLS, while the larval survival rate, pupation rate, and pupa weight decreased. Notably, when feeding dsRNA-encapsulated carbon quantum dots (CQD-dsRNA), this trend was significantly strengthened (<i>p</i> &lt; 0.05). Taken together, these findings provide evidence supporting HaSumf1's conservation involvement in the adaptation of host plant GLSs during lepidopteran insect evolution. Therefore, it plays a critical role in the growth and development of <i>H. armigera</i>, offering molecular targets for developing novel pesticides and targeted control measures against this vital pest.</p>\",\"PeriodicalId\":14987,\"journal\":{\"name\":\"Journal of Applied Entomology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2024-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Applied Entomology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/jen.13236\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENTOMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Applied Entomology","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jen.13236","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

食草昆虫体内的古柯碱硫酸酯硫酸酯酶-硫酸酯酶修饰因子(Sumfs)系统通过迅速脱硫,在保护寄主植物葡萄糖苷酸盐(GLSs)--十字花科植物的化合物--方面发挥着重要功能。此前,在 Helicoverpa armigera 中发现了 8 种 HaSulfs 和 1 种 HaSumf1。GLS对H. armigera幼虫的生长发育有重大影响。它们能在基因和蛋白质水平上诱导 H. armigera 中肠中 HaSulf 的表达。在本研究中,我们利用 RNAi 技术沉默了 HaSumf1 基因,并探讨了其对 GLS 生长、发育和适应性的潜在影响。序列分析表明,H. armigera 的 HaSumf1 基因与其他昆虫基因具有高度的序列同一性,并且在第五龄期表达水平最高。此外,qRT-PCR 结果显示,当喂食 0.004% 和 0.04% 的 GLS 时,HaSumf1 的表达在三龄幼虫期分别上调了 1.26 倍和 0.07 倍,在五龄幼虫期分别上调了 1.65 倍和 0.19 倍。dsSumf1沉默的效果在三龄幼虫和五龄幼虫阶段都能观察到,沉默率分别为65.5%-88.9%和61.3%-74.6%(p <0.05)。生物测定结果表明,用 0.004% GLS 和 0.04% GLS 处理后,H. armigera 五龄体重显著增加,而幼虫存活率、化蛹率和蛹重均下降。值得注意的是,当喂食dsRNA封装的碳量子点(CQD-dsRNA)时,这一趋势明显加强(p < 0.05)。综上所述,这些发现为 HaSumf1 在鳞翅目昆虫进化过程中参与寄主植物 GLS 的适应性保护提供了证据。因此,HaSumf1 在 H. armigera 的生长发育过程中起着关键作用,为开发新型杀虫剂和针对这种重要害虫的定向控制措施提供了分子靶标。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Functions of the sulfatase-modifying factor 1 (HaSumf1) in the development and host glucosinolate adaptation of Helicoverpa armigera

The system of gulcosinoalte sulfatase–sulfatase-modifying factors (Sumfs) in herbivorous insects fulfils an essential function in defending host plant glucosinolates (GLSs)—the compounds of cruciferous plants—by swiftly desulfurising them. Previously, eight HaSulfs and one HaSumf1 were identified in Helicoverpa armigera. GLSs have a significant impact on the growth and development of H. armigera larvae. They can induce the expression of HaSulf in the midgut of H. armigera at both gene and protein levels. In the present study, we utilised RNAi technology to silence the HaSumf1 gene and explored its potential impact on the growth, development, and adaptation of GLSs. The sequence analysis has indicated that the HaSumf1 gene in H. armigera shares a high degree of sequence identity with other insect genes and is expressed at its highest level in the fifth-instar phase. Additionally, the qRT-PCR results reveal that the expression of HaSumf1 was upregulated by 1.26 and 0.07 times in third-instar and 1.65 and 0.19 times in fifth-instar when fed with 0.004% and 0.04% GLS, respectively. The effectiveness of dsSumf1 silencing was observed in both third- and fifth-instar stages, with silencing rates of 65.5%–88.9% and 61.3%–74.6%, respectively (p < 0.05). The findings of the bioassay indicate that H. armigera's body weight at fifth-instar significantly increased after treatment with 0.004% GLS and 0.04% GLS, while the larval survival rate, pupation rate, and pupa weight decreased. Notably, when feeding dsRNA-encapsulated carbon quantum dots (CQD-dsRNA), this trend was significantly strengthened (p < 0.05). Taken together, these findings provide evidence supporting HaSumf1's conservation involvement in the adaptation of host plant GLSs during lepidopteran insect evolution. Therefore, it plays a critical role in the growth and development of H. armigera, offering molecular targets for developing novel pesticides and targeted control measures against this vital pest.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
3.40
自引率
5.30%
发文量
132
审稿时长
6 months
期刊介绍: The Journal of Applied Entomology publishes original articles on current research in applied entomology, including mites and spiders in terrestrial ecosystems. Submit your next manuscript for rapid publication: the average time is currently 6 months from submission to publication. With Journal of Applied Entomology''s dynamic article-by-article publication process, Early View, fully peer-reviewed and type-set articles are published online as soon as they complete, without waiting for full issue compilation.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信