南极多毛目环节动物 Microspio moorei(匙吻鲟科)的全新转录组测序和注释及其热应激相关蛋白(HSP、SOD 和 CAT)的特征描述

IF 1.3 4区 生物学 Q4 GENETICS & HEREDITY
Idalyd Fonseca-González , Esteban Velasquez-Agudelo , Mario H. Londoño-Mesa , Javier C. Álvarez
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引用次数: 0

摘要

我们介绍了2017年在南极半岛乔治王岛菲尔德斯湾的南极野外考察中收集到的非模式南极多毛类蠕虫Microspio moorei(刺毛虫科)的全新转录组组装。在此,我们报告了首个针对Microspio spp.的转录组参考阵列,从多种功能(即生物和代谢过程、催化过程和催化活性)对该刺尾虫的基因序列进行了注释。将 HSP70、HSP90 SOD 和 CAT 家族与已报道的环节动物转录组和蛋白质组进行了比较。利用 COI、16S 和 18S 标记进行的系统进化分析有效地将物种聚类在该家族中。不过,它也对小栉水母属的单系性质提出了不确定性,表明有必要获得更多数据,并可能需要对其分组进行重新评估。在这些蛋白质家族中,使用三维模型软件创建了一个具有代表性的蛋白质结构。结构预测结果与相关报道的生活在不同温度下的环带动物和人类 X 射线参照物进行了比较。我们发现人类 HSP 蛋白之间存在结构差异(RMSE >1.8),但多毛目动物预测的蛋白质之间没有明显差异(RMSE <1.2)。这些结果鼓励了对热应激相关蛋白质的进一步研究,鼓励了对气候变化诱导的温度应激的遗传标记的开发,以及对热反应潜在机制的研究。此外,这些结果还有助于将这些发现推广到同属物种。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
De novo transcriptome sequencing and annotation of the Antarctic polychaete Microspio moorei (Spionidae) with its characterization of the heat stress-related proteins (HSP, SOD & CAT)

We present a de novo transcriptome assembly for the non-model Antarctic polychaete worm Microspio moorei (Spionidae) collected during Antarctic field expedition in Fildes Bay, King George Island, Antarctic Peninsula, in 2017. Here, we report the first transcriptome reference array for Microspio spp. The gene sequences of the spionid worm were annotated from a wide range of functions (i.e., biological, and metabolic processes, catalytic processes, and catalytic activity). HSP70, HSP90 SOD and CAT families were compared to reported annelid transcriptomes and proteomes. The phylogenetic analysis using COI, 16S, and 18S markers effectively clusters the species within the family. However, it also casts uncertainty on the monophyletic nature of the Microspio genera, indicating the necessity for additional data and potentially requiring a reevaluation of its grouping. Within these protein families, 3D model software was used to create one representative of their protein structures. Structural predictions were compared with related reported annelids living at different temperatures and a human X-ray reference. We found structural differences (RMSE >1.8) between the human HSP proteins but no significant differences between the polychaete-predicted proteins (RMSE <1.2). These results encourage further research of heat stress-related proteins, the development of genetic markers for climate change-induced temperature stress, and the study of the underlying mechanisms of the heat response. Moreover, these results motivate the extension of these findings to congeneric species.

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来源期刊
Marine genomics
Marine genomics 生物-遗传学
CiteScore
3.60
自引率
5.30%
发文量
50
审稿时长
29 days
期刊介绍: The journal publishes papers on all functional and evolutionary aspects of genes, chromatin, chromosomes and (meta)genomes of marine (and freshwater) organisms. It deals with new genome-enabled insights into the broader framework of environmental science. Topics within the scope of this journal include: • Population genomics and ecology • Evolutionary and developmental genomics • Comparative genomics • Metagenomics • Environmental genomics • Systems biology More specific topics include: geographic and phylogenomic characterization of aquatic organisms, metabolic capacities and pathways of organisms and communities, biogeochemical cycles, genomics and integrative approaches applied to microbial ecology including (meta)transcriptomics and (meta)proteomics, tracking of infectious diseases, environmental stress, global climate change and ecosystem modelling.
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