在小鼠神经发育过程中,Kcnk9的印记表达先于双拷贝非生产性增强子-启动子相互作用。

IF 3.3 Q2 GENETICS & HEREDITY
HGG Advances Pub Date : 2024-04-11 Epub Date: 2024-01-30 DOI:10.1016/j.xhgg.2024.100271
Cecilia Rengifo Rojas, Jil Cercy, Sophie Perillous, Céline Gonthier-Guéret, Bertille Montibus, Stéphanie Maupetit-Méhouas, Astrid Espinadel, Marylou Dupré, Charles C Hong, Kenichiro Hata, Kazuhiko Nakabayashi, Antonius Plagge, Tristan Bouschet, Philippe Arnaud, Isabelle Vaillant, Franck Court
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引用次数: 0

摘要

人们对印记控制区(ICR)的等位基因构成性甲基化如何与其他调控水平相互作用,从而驱动大型印记结构域的亲本等位基因特异性及时表达还只是部分了解。Peg13-Kcnk9 结构域是一个具有重要脑功能的印记结构域。为了深入了解其在神经承载过程中的调控,我们利用基于小鼠干细胞的皮质发育模型对其等位基因特异性表观遗传、转录组和顺式空间组织进行了综合分析,该模型再现了神经发育过程中印记基因表达的调控。我们发现,尽管存在与父系 CTCF 结合的 Peg13 ICR 相关的等位基因高阶染色质结构,但增强子-Kcnk9 启动子接触在两个等位基因上都有发生,尽管它们只在母系等位基因上产生作用。这一观察结果对 CTCF 结合将增强子及其目标基因隔离在两侧的典型模型提出了挑战,并表明等位基因 CTCF 结合在某些 ICR 上发挥着更微妙的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Biallelic non-productive enhancer-promoter interactions precede imprinted expression of Kcnk9 during mouse neural commitment.

It is only partially understood how constitutive allelic methylation at imprinting control regions (ICRs) interacts with other regulation levels to drive timely parental allele-specific expression along large imprinted domains. The Peg13-Kcnk9 domain is an imprinted domain with important brain functions. To gain insights into its regulation during neural commitment, we performed an integrative analysis of its allele-specific epigenetic, transcriptomic, and cis-spatial organization using a mouse stem cell-based corticogenesis model that recapitulates the control of imprinted gene expression during neurodevelopment. We found that, despite an allelic higher-order chromatin structure associated with the paternally CTCF-bound Peg13 ICR, enhancer-Kcnk9 promoter contacts occurred on both alleles, although they were productive only on the maternal allele. This observation challenges the canonical model in which CTCF binding isolates the enhancer and its target gene on either side and suggests a more nuanced role for allelic CTCF binding at some ICRs.

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来源期刊
HGG Advances
HGG Advances Biochemistry, Genetics and Molecular Biology-Molecular Medicine
CiteScore
4.30
自引率
4.50%
发文量
69
审稿时长
14 weeks
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