Bingyu Jiao, Le Wang, Haitao Gui, Zifu Ni, Rong Du, Yuansen Hu
{"title":"利用深层共晶溶剂绿色提取玉米芯木聚糖的研究","authors":"Bingyu Jiao, Le Wang, Haitao Gui, Zifu Ni, Rong Du, Yuansen Hu","doi":"10.1016/j.gaost.2024.01.003","DOIUrl":null,"url":null,"abstract":"<div><p>Corn as one of the world's major food crops, its by-product corn cob is also rich in resources. However, the unreasonable utilization of corn cob often causes the environmental pollution, waste of resources and other problems. As one of the most abundant polymers in nature, xylan is widely used in food, medicine, materials and other fields. Corn cob is rich in xylan, which is an ideal raw material for extracting xylan. However, the intractable lignin is covalently linked to xylan, which increases the difficulty of xylan extraction. It has been reported that the deep eutectic solvent (DES) could preferentially dissolve lignin in biomass, thereby dissolving the xylan. Then, the xylan in the extract was separated by ethanol precipitation method. The xylan precipitate was obtained after centrifugation, while the supernatant was retained. The components of the supernatant after ethanol precipitation were separated by the rotary evaporator. The ethanol, water and DES were collected for the subsequent extraction of corn cob xylan. In this study, a novel way was provided for the green production of corn cob xylan. The DES was used to extract xylan from corn cob which was used as the raw material. The effects of solid-liquid ratio, reaction time, reaction temperature and water content of DES on the extraction rate of corn cob xylan were investigated by the single factor test. Furthermore, the orthogonal test was designed to optimize the xylan extraction process. The structure of corn cob xylan was analyzed and verified. The results showed that the optimum extraction conditions of corn cob xylan were as follows: the ratio of corn cob to DES was 1 : 15 (g : mL), the extraction time was 3 h, the extraction temperature was 60 °C, and the water content of DES was 70%. Under these conditions, the extraction rate of xylan was 16.46%. The extracted corn cob xylan was distinctive triple helix of polysaccharide, which was similar to the structure of commercially available xylan. Xylan was effectively and workably extracted from corn cob by the DES method. This study provided a new approach for high value conversion of corn cob and the clean production of xylan.</p></div>","PeriodicalId":33614,"journal":{"name":"Grain Oil Science and Technology","volume":"7 1","pages":"Pages 50-59"},"PeriodicalIF":0.0000,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590259824000037/pdfft?md5=dbe8cacfea64a65bcfd591ab1900fd9f&pid=1-s2.0-S2590259824000037-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Study on the green extraction of corncob xylan by deep eutectic solvent\",\"authors\":\"Bingyu Jiao, Le Wang, Haitao Gui, Zifu Ni, Rong Du, Yuansen Hu\",\"doi\":\"10.1016/j.gaost.2024.01.003\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Corn as one of the world's major food crops, its by-product corn cob is also rich in resources. However, the unreasonable utilization of corn cob often causes the environmental pollution, waste of resources and other problems. As one of the most abundant polymers in nature, xylan is widely used in food, medicine, materials and other fields. Corn cob is rich in xylan, which is an ideal raw material for extracting xylan. However, the intractable lignin is covalently linked to xylan, which increases the difficulty of xylan extraction. It has been reported that the deep eutectic solvent (DES) could preferentially dissolve lignin in biomass, thereby dissolving the xylan. Then, the xylan in the extract was separated by ethanol precipitation method. The xylan precipitate was obtained after centrifugation, while the supernatant was retained. The components of the supernatant after ethanol precipitation were separated by the rotary evaporator. The ethanol, water and DES were collected for the subsequent extraction of corn cob xylan. In this study, a novel way was provided for the green production of corn cob xylan. The DES was used to extract xylan from corn cob which was used as the raw material. The effects of solid-liquid ratio, reaction time, reaction temperature and water content of DES on the extraction rate of corn cob xylan were investigated by the single factor test. Furthermore, the orthogonal test was designed to optimize the xylan extraction process. The structure of corn cob xylan was analyzed and verified. The results showed that the optimum extraction conditions of corn cob xylan were as follows: the ratio of corn cob to DES was 1 : 15 (g : mL), the extraction time was 3 h, the extraction temperature was 60 °C, and the water content of DES was 70%. Under these conditions, the extraction rate of xylan was 16.46%. The extracted corn cob xylan was distinctive triple helix of polysaccharide, which was similar to the structure of commercially available xylan. Xylan was effectively and workably extracted from corn cob by the DES method. This study provided a new approach for high value conversion of corn cob and the clean production of xylan.</p></div>\",\"PeriodicalId\":33614,\"journal\":{\"name\":\"Grain Oil Science and Technology\",\"volume\":\"7 1\",\"pages\":\"Pages 50-59\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2590259824000037/pdfft?md5=dbe8cacfea64a65bcfd591ab1900fd9f&pid=1-s2.0-S2590259824000037-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Grain Oil Science and Technology\",\"FirstCategoryId\":\"1087\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2590259824000037\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Grain Oil Science and Technology","FirstCategoryId":"1087","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2590259824000037","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 0
摘要
玉米作为世界主要粮食作物之一,其副产品玉米芯也蕴含着丰富的资源。然而,玉米芯的不合理利用往往会造成环境污染、资源浪费等问题。木聚糖是自然界中含量最丰富的聚合物之一,被广泛应用于食品、医药、材料等领域。玉米芯富含木聚糖,是提取木聚糖的理想原料。然而,难以处理的木质素与木聚糖共价连接,增加了提取木聚糖的难度。据报道,深共晶溶剂(DES)可优先溶解生物质中的木质素,从而溶解木聚糖。然后,用乙醇沉淀法分离提取物中的木聚糖。离心后得到木聚糖沉淀,保留上清液。乙醇沉淀后的上清液中的成分用旋转蒸发仪分离。收集乙醇、水和 DES,用于后续提取玉米芯木聚糖。本研究为玉米芯木聚糖的绿色生产提供了一种新方法。使用 DES 从作为原料的玉米芯中提取木聚糖。通过单因素试验研究了DES的固液比、反应时间、反应温度和含水量对玉米芯木聚糖提取率的影响。此外,还设计了正交试验来优化木聚糖提取工艺。对玉米芯木聚糖的结构进行了分析和验证。结果表明,玉米芯木聚糖的最佳提取条件为:玉米芯与 DES 的比例为 1 : 15(g : mL),提取时间为 3 h,提取温度为 60 °C,DES 的含水量为 70%。在此条件下,木聚糖的提取率为 16.46%。提取的玉米芯木聚糖为明显的三螺旋多糖,与市售的木聚糖结构相似。采用 DES 方法从玉米芯中提取木聚糖是有效且可行的。这项研究为玉米芯的高价值转化和木聚糖的清洁生产提供了一种新方法。
Study on the green extraction of corncob xylan by deep eutectic solvent
Corn as one of the world's major food crops, its by-product corn cob is also rich in resources. However, the unreasonable utilization of corn cob often causes the environmental pollution, waste of resources and other problems. As one of the most abundant polymers in nature, xylan is widely used in food, medicine, materials and other fields. Corn cob is rich in xylan, which is an ideal raw material for extracting xylan. However, the intractable lignin is covalently linked to xylan, which increases the difficulty of xylan extraction. It has been reported that the deep eutectic solvent (DES) could preferentially dissolve lignin in biomass, thereby dissolving the xylan. Then, the xylan in the extract was separated by ethanol precipitation method. The xylan precipitate was obtained after centrifugation, while the supernatant was retained. The components of the supernatant after ethanol precipitation were separated by the rotary evaporator. The ethanol, water and DES were collected for the subsequent extraction of corn cob xylan. In this study, a novel way was provided for the green production of corn cob xylan. The DES was used to extract xylan from corn cob which was used as the raw material. The effects of solid-liquid ratio, reaction time, reaction temperature and water content of DES on the extraction rate of corn cob xylan were investigated by the single factor test. Furthermore, the orthogonal test was designed to optimize the xylan extraction process. The structure of corn cob xylan was analyzed and verified. The results showed that the optimum extraction conditions of corn cob xylan were as follows: the ratio of corn cob to DES was 1 : 15 (g : mL), the extraction time was 3 h, the extraction temperature was 60 °C, and the water content of DES was 70%. Under these conditions, the extraction rate of xylan was 16.46%. The extracted corn cob xylan was distinctive triple helix of polysaccharide, which was similar to the structure of commercially available xylan. Xylan was effectively and workably extracted from corn cob by the DES method. This study provided a new approach for high value conversion of corn cob and the clean production of xylan.