Sho Usuki, S. Machida, Ken‐ichi Katsumata, Makoto Ogawa, S. S. Latthe, Shanhu Liu, Kenji Yamatoya, Kazuya Nakata
{"title":"使用地面掺杂 Rh 的 SrTiO3 光催化剂在大肠杆菌存在的情况下选择性灭活 Qβ 噬菌体的机制","authors":"Sho Usuki, S. Machida, Ken‐ichi Katsumata, Makoto Ogawa, S. S. Latthe, Shanhu Liu, Kenji Yamatoya, Kazuya Nakata","doi":"10.3390/catal14020094","DOIUrl":null,"url":null,"abstract":"Photocatalysts have recently attracted attention for removing infectious-disease-causing bacteria and viruses. Among such photocatalysts, ground Rh-doped SrTiO3 (“g-STO:Rh”) has been found to have biospecificity that reduces the Qβ phage infectivity under conditions that did not decrease the E. coli survival rate. Elucidating the mechanism of selective antiphage activation is important for developing photocatalysts that act effectively against specific microorganisms. In this study, SDS-PAGE and quantitative PCR showed that a g-STO:Rh-treated Qβ phage preferentially inactivated the A2 protein involved in attachment to host cells. The analysis of the photocatalyst-treated ovalbumin using g-STO:Rh indicated that the protein’s isoelectric point significantly influenced the initial interaction with g-STO:Rh. However, once the protein is absorbed, it was decomposed without the release of intermediates. Furthermore, an inactivation assay for four different phages by photocatalyst treatment using g-STO:Rh revealed that phages with positively charged proteins are highly susceptible to inactivation, and the accessibility of critical components to g-STO:Rh influences susceptibility. We conclude that the selective antiphage activation of g-STO:Rh depends on the adsorption efficiency of the protein and g-STO:Rh.","PeriodicalId":3,"journal":{"name":"ACS Applied Electronic Materials","volume":"43 3","pages":""},"PeriodicalIF":4.3000,"publicationDate":"2024-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Mechanism of Selective Qβ Bacteriophage Inactivation under the Presence of E. Coli Using Ground Rh-Doped SrTiO3 Photocatalyst\",\"authors\":\"Sho Usuki, S. Machida, Ken‐ichi Katsumata, Makoto Ogawa, S. S. Latthe, Shanhu Liu, Kenji Yamatoya, Kazuya Nakata\",\"doi\":\"10.3390/catal14020094\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Photocatalysts have recently attracted attention for removing infectious-disease-causing bacteria and viruses. Among such photocatalysts, ground Rh-doped SrTiO3 (“g-STO:Rh”) has been found to have biospecificity that reduces the Qβ phage infectivity under conditions that did not decrease the E. coli survival rate. Elucidating the mechanism of selective antiphage activation is important for developing photocatalysts that act effectively against specific microorganisms. In this study, SDS-PAGE and quantitative PCR showed that a g-STO:Rh-treated Qβ phage preferentially inactivated the A2 protein involved in attachment to host cells. The analysis of the photocatalyst-treated ovalbumin using g-STO:Rh indicated that the protein’s isoelectric point significantly influenced the initial interaction with g-STO:Rh. However, once the protein is absorbed, it was decomposed without the release of intermediates. Furthermore, an inactivation assay for four different phages by photocatalyst treatment using g-STO:Rh revealed that phages with positively charged proteins are highly susceptible to inactivation, and the accessibility of critical components to g-STO:Rh influences susceptibility. We conclude that the selective antiphage activation of g-STO:Rh depends on the adsorption efficiency of the protein and g-STO:Rh.\",\"PeriodicalId\":3,\"journal\":{\"name\":\"ACS Applied Electronic Materials\",\"volume\":\"43 3\",\"pages\":\"\"},\"PeriodicalIF\":4.3000,\"publicationDate\":\"2024-01-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Applied Electronic Materials\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.3390/catal14020094\",\"RegionNum\":3,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENGINEERING, ELECTRICAL & ELECTRONIC\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Electronic Materials","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.3390/catal14020094","RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, ELECTRICAL & ELECTRONIC","Score":null,"Total":0}
Mechanism of Selective Qβ Bacteriophage Inactivation under the Presence of E. Coli Using Ground Rh-Doped SrTiO3 Photocatalyst
Photocatalysts have recently attracted attention for removing infectious-disease-causing bacteria and viruses. Among such photocatalysts, ground Rh-doped SrTiO3 (“g-STO:Rh”) has been found to have biospecificity that reduces the Qβ phage infectivity under conditions that did not decrease the E. coli survival rate. Elucidating the mechanism of selective antiphage activation is important for developing photocatalysts that act effectively against specific microorganisms. In this study, SDS-PAGE and quantitative PCR showed that a g-STO:Rh-treated Qβ phage preferentially inactivated the A2 protein involved in attachment to host cells. The analysis of the photocatalyst-treated ovalbumin using g-STO:Rh indicated that the protein’s isoelectric point significantly influenced the initial interaction with g-STO:Rh. However, once the protein is absorbed, it was decomposed without the release of intermediates. Furthermore, an inactivation assay for four different phages by photocatalyst treatment using g-STO:Rh revealed that phages with positively charged proteins are highly susceptible to inactivation, and the accessibility of critical components to g-STO:Rh influences susceptibility. We conclude that the selective antiphage activation of g-STO:Rh depends on the adsorption efficiency of the protein and g-STO:Rh.
期刊介绍:
ACS Applied Electronic Materials is an interdisciplinary journal publishing original research covering all aspects of electronic materials. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials science, engineering, optics, physics, and chemistry into important applications of electronic materials. Sample research topics that span the journal's scope are inorganic, organic, ionic and polymeric materials with properties that include conducting, semiconducting, superconducting, insulating, dielectric, magnetic, optoelectronic, piezoelectric, ferroelectric and thermoelectric.
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