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引用次数: 0
摘要
摘要 背景 Platycladus orientalis (L.) Franco（PO）在东方医学中被用于治疗风湿和腹泻，但其抗痛作用仍不明确。 目的 本研究旨在揭示 PO 提取物的抗痛觉作用，并对其在 RAW 264.7 细胞和单钠尿酸（MSU）诱导的小鼠疼痛中的作用进行标准化。 结果 使用不同浓度（0-100%）的乙醇（EtOH）制备 PO 提取物。然后，根据 MSU 诱导的小鼠疼痛模型和使用脂多糖（LPS）诱导的 RAW 264.7 细胞进行一氧化氮（NO）检测的结果确定组别。由于二氯甲烷（CH2Cl2）层和乙酸乙酯（EtOAc）层随着时间的推移显示出越来越大的作用，因此选择这两层来分离主要成分。使用高效的 HPLC 方法对 PO 提取物进行了标准化，并使用 von Frey 试验测定了其在 MSU 诱导的痛风疼痛小鼠模型中的抗痛风作用。结果发现 PO 提取物具有抗痛风作用。此外，在对这些化合物进行验证的基础上，成功实现了样品标准化。 结论 PO 提取物在痛风疼痛的动物模型中表现出了强效的剂量和时间依赖性抗痛风作用；这些作用可能是由该提取物的主要化合物介导的。总之，这些结果表明，PO 可用作治疗痛风相关疼痛的草药。
Evaluation of the antinociceptive effect and standardization of Platycladus orientalis (L.) Franco extract
Abstract
Background
Platycladus orientalis (L.) Franco (PO) has been used in Oriental medicine to treat rheumatism and diarrhea; however, its antinociceptive effects remain unclear.
Objective
This study aimed to reveal the antinociceptive effect of the PO extract and standardize this extract in RAW 264.7 cells and mice with monosodium uric acid (MSU)-induced pain.
Results
PO extracts were prepared using different ethanol (EtOH) concentrations (0–100%). Thereafter, groups were determined based on the results of the MSU-induced pain model in mice and nitric oxide (NO) assay using lipopolysaccharide (LPS)-induced RAW 264.7 cells. As the methylene chloride (CH2Cl2) and ethyl acetate (EtOAc) layers exhibited increasing effects with time, these layers were selected to isolate the major components. The PO extract was standardized using a highly efficient HPLC method, and its antinociceptive effects in a mouse model of MSU-induced gout pain were determined using the von Frey test. The PO extract was found to exhibit antinociceptive effects. Moreover, sample standardization was successfully achieved based on the validation of these compounds.
Conclusion
The PO extract exhibited potent dose- and time-dependent antinociceptive effects in an animal model of gout pain; these effects might be mediated by the major compounds of this extract. Collectively, these results suggest that PO can be used as a herbal drug to treat gout-related pain.
期刊介绍：
Molecular & Cellular Toxicology publishes original research and reviews in all areas of the complex interaction between the cell´s genome (the sum of all genes within the chromosome), chemicals in the environment, and disease. Acceptable manuscripts are the ones that deal with some topics of environmental contaminants, including those that lie in the domains of analytical chemistry, biochemistry, pharmacology and toxicology with the aspects of molecular and cellular levels. Emphasis will be placed on toxic effects observed at relevant genomics and proteomics, which have direct impact on drug development, environment health, food safety, preventive medicine, and forensic medicine. The journal is committed to rapid peer review to ensure the publication of highest quality original research and timely news and review articles.
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