Michael F. Espenship, Gregory Eakins, Julia Laskin
{"title":"用于旋转电场质量分析仪的多通道锁相波形发生器","authors":"Michael F. Espenship, Gregory Eakins, Julia Laskin","doi":"10.1016/j.ijms.2024.117205","DOIUrl":null,"url":null,"abstract":"<div><p><span>We have developed a multichannel phase-locked sine waveform generator for a mass dispersive rotating electric field device, known as a rotating wall mass analyzer (RWMA). The waveform generator outputs eight sine waves phase shifted by 45°. The frequency of the waveforms is adjustable in the range of 0.1–100 kHz and the maximum amplitude is 100 V</span><sub>p-p</sub>, which is greater than 20 V<sub>p-p</sub><span> provided by commercial arbitrary waveform generators. Adjustment of either amplitude or frequency maintains phase alignment for all eight output channels to continually generate a uniform rotating electric field inside the RWMA. The RWMA is a high-throughput mass analyzer for preparative mass spectrometry<span> that separates a multicomponent continuous ion beam in space and enables the deposition of ions of different </span></span><em>m/z</em> onto concentric ring shapes on surfaces. Here, we demonstrate that increasing the generator's amplitude from 20 V<sub>p-p</sub> to 50 V<sub>p-p</sub> resolves a continuous ion beam of ubiquitin charge states with a measured mass resolution (m/Δm) of R = 14. This resolution improves to R = 26 for a 100 V<sub>p-p</sub> generator output. Furthermore, the designed waveform generator facilitates a single <em>m/z</em> calibration of the rotating electric field parameters to extract a <em>m/z</em> value from a measured radius. These parameters are shown to be valid for other <em>m/z</em> species, ion beam kinetic energies, and ionization polarity modes.</p></div>","PeriodicalId":338,"journal":{"name":"International Journal of Mass Spectrometry","volume":null,"pages":null},"PeriodicalIF":1.6000,"publicationDate":"2024-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A multichannel phase-locked waveform generator for a rotating electric field mass analyzer\",\"authors\":\"Michael F. Espenship, Gregory Eakins, Julia Laskin\",\"doi\":\"10.1016/j.ijms.2024.117205\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span>We have developed a multichannel phase-locked sine waveform generator for a mass dispersive rotating electric field device, known as a rotating wall mass analyzer (RWMA). The waveform generator outputs eight sine waves phase shifted by 45°. The frequency of the waveforms is adjustable in the range of 0.1–100 kHz and the maximum amplitude is 100 V</span><sub>p-p</sub>, which is greater than 20 V<sub>p-p</sub><span> provided by commercial arbitrary waveform generators. Adjustment of either amplitude or frequency maintains phase alignment for all eight output channels to continually generate a uniform rotating electric field inside the RWMA. The RWMA is a high-throughput mass analyzer for preparative mass spectrometry<span> that separates a multicomponent continuous ion beam in space and enables the deposition of ions of different </span></span><em>m/z</em> onto concentric ring shapes on surfaces. Here, we demonstrate that increasing the generator's amplitude from 20 V<sub>p-p</sub> to 50 V<sub>p-p</sub> resolves a continuous ion beam of ubiquitin charge states with a measured mass resolution (m/Δm) of R = 14. This resolution improves to R = 26 for a 100 V<sub>p-p</sub> generator output. Furthermore, the designed waveform generator facilitates a single <em>m/z</em> calibration of the rotating electric field parameters to extract a <em>m/z</em> value from a measured radius. These parameters are shown to be valid for other <em>m/z</em> species, ion beam kinetic energies, and ionization polarity modes.</p></div>\",\"PeriodicalId\":338,\"journal\":{\"name\":\"International Journal of Mass Spectrometry\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2024-01-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Mass Spectrometry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1387380624000162\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"PHYSICS, ATOMIC, MOLECULAR & CHEMICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Mass Spectrometry","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1387380624000162","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PHYSICS, ATOMIC, MOLECULAR & CHEMICAL","Score":null,"Total":0}
A multichannel phase-locked waveform generator for a rotating electric field mass analyzer
We have developed a multichannel phase-locked sine waveform generator for a mass dispersive rotating electric field device, known as a rotating wall mass analyzer (RWMA). The waveform generator outputs eight sine waves phase shifted by 45°. The frequency of the waveforms is adjustable in the range of 0.1–100 kHz and the maximum amplitude is 100 Vp-p, which is greater than 20 Vp-p provided by commercial arbitrary waveform generators. Adjustment of either amplitude or frequency maintains phase alignment for all eight output channels to continually generate a uniform rotating electric field inside the RWMA. The RWMA is a high-throughput mass analyzer for preparative mass spectrometry that separates a multicomponent continuous ion beam in space and enables the deposition of ions of different m/z onto concentric ring shapes on surfaces. Here, we demonstrate that increasing the generator's amplitude from 20 Vp-p to 50 Vp-p resolves a continuous ion beam of ubiquitin charge states with a measured mass resolution (m/Δm) of R = 14. This resolution improves to R = 26 for a 100 Vp-p generator output. Furthermore, the designed waveform generator facilitates a single m/z calibration of the rotating electric field parameters to extract a m/z value from a measured radius. These parameters are shown to be valid for other m/z species, ion beam kinetic energies, and ionization polarity modes.
期刊介绍:
The journal invites papers that advance the field of mass spectrometry by exploring fundamental aspects of ion processes using both the experimental and theoretical approaches, developing new instrumentation and experimental strategies for chemical analysis using mass spectrometry, developing new computational strategies for data interpretation and integration, reporting new applications of mass spectrometry and hyphenated techniques in biology, chemistry, geology, and physics.
Papers, in which standard mass spectrometry techniques are used for analysis will not be considered.
IJMS publishes full-length articles, short communications, reviews, and feature articles including young scientist features.