左氧氟沙星和三甲氧苄氨嘧啶-磺胺甲噁唑对嗜麦芽糖单胞菌生物膜的根除作用

IF 1.3 4区 医学 Q4 INFECTIOUS DISEASES
José Mauricio Del Río-Chacón, Fabián Rojas-Larios, Paola Bocanegra-Ibarias, Daniel Salas-Treviño, Francisco Espinoza-Gómez, Adrián Camacho-Ortiz, Samantha Flores-Treviño
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引用次数: 0

摘要

嗜麦芽气单胞菌是一种非发酵革兰氏阴性耐药病原体,可引起医疗相关感染。通过水晶紫染色法评估了来自墨西哥的临床分离株的生物膜生成情况。采用肉汤微稀释法对浮游细胞和生物膜细胞进行了抗菌药敏感性评估。通过荧光显微镜观察抗生素对生物膜的影响。研究共纳入了 50 个分离株,其中 28.0% 为生物膜产生株(64.2% 来自血液样本,35.7% 来自呼吸道样本)。浮游细胞对左氧氟沙星(8.0%)和三甲氧苄氨嘧啶-磺胺甲噁唑(44.0%)的耐药性在生物膜细胞中增至 100%。用几种浓度的这两种抗生素处理的细菌生物膜被完全破坏。总之,从血液中分离出的嗜麦芽糖酵母菌比从呼吸道样本中分离出的嗜麦芽糖酵母菌产生更多的生物膜。生物膜的产生增加了对抗生素的耐药性。在墨西哥,单一抗生素疗法可能不是治疗嗜麦芽糖酵母菌感染的最佳方案,因为它们也可能导致生物膜的产生。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Biofilm eradication of Stenotrophomonas maltophilia by Levofloxacin and Trimethoprim-sulfamethoxazole

Stenotrophomonas maltophilia is a non-fermenting Gram-negative drug-resistant pathogen causing healthcare-associated infections. Clinical isolates from Mexico were assessed for biofilm production by crystal violet staining. Antimicrobial susceptibility was evaluated using the broth microdilution method in planktonic and biofilm cells. The effect of antibiotics on the biofilm was visualized by fluorescence microscopy. Fifty isolates were included in the study, of which 28.0% were biofilm producers (64.2% from blood and 35.7% from respiratory samples). Resistance to levofloxacin (8.0%) and trimethoprim-sulfamethoxazole (44.0%) in planktonic cells increased to 100% in biofilm cells. Bacterial biofilm treated with several concentrations of both antibiotics was completely disrupted. In conclusion, S. maltophilia isolated from blood had higher biofilm production than those from respiratory samples. Resistance to antibiotics increased due to biofilm production. Antibiotic monotherapy might not be the best course of action for the treatment of S. maltophilia infections in Mexico, as they might also be causing biofilm production.

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来源期刊
CiteScore
4.50
自引率
4.50%
发文量
172
审稿时长
2 months
期刊介绍: Japanese Journal of Infectious Diseases (JJID), an official bimonthly publication of National Institute of Infectious Diseases, Japan, publishes papers dealing with basic research on infectious diseases relevant to humans in the fields of bacteriology, virology, mycology, parasitology, medical entomology, vaccinology, and toxinology. Pathology, immunology, biochemistry, and blood safety related to microbial pathogens are among the fields covered. Sections include: original papers, short communications, epidemiological reports, methods, laboratory and epidemiology communications, letters to the editor, and reviews.
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